Parasite infiltrates fruitfly research

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Geneticists fear Drosophila results skewed by unnoticed bacterium.

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The eggs of infected fruitflies spread the Wolbachia bacterium, which can hide the effects of mutations. Credit: OSF/PHOTOLIBRARY.COM

An unnoticed parasite could be scuppering fly geneticists' experiments. Up to a third of laboratory strains of their favourite test organism — the fruitfly Drosophila melanogaster — are infected with a parasite that affects the flies' biology, according to a study of hundreds of stocks of the fly. The finding suggests that the bacterium could be silently affecting the outcome of a significant number of studies.

The parasite, Wolbachia, can only be transmitted through infected eggs, and has evolved strategies for maximizing its transmission, such as switching the sex of male embryos. It can reduce Drosophila sperm and egg production, influence longevity and — critically for experiments — shelter its host from the effects of harmful mutations.

One mutation in a gene called Sex-lethal prevents female flies from producing eggs, for example, but Wolbachia-infected females carrying this mutation produce near-normal numbers of eggs1. Certain strains with differing lifespans live for the same length of time after being treated with the antibiotic tetracycline, which kills Wolbachia2.

Yet Drosophila researchers rarely consider Wolbachia's effects, according to Timothy Karr of the University of Bath, UK. “It's amazing how many people don't know this,” he says.

Karr and his team surveyed 609 of the mutant strains housed at the Bloomington Stock Center at Indiana University for Wolbachia genes. They picked a wide range that included strains with large DNA deletions called deficiencies, smaller ‘point’ mutations, and mutations created with mobile pieces of DNA called P-elements, as well as normal, or ‘wild-type’, stocks. Some 30% of strains were infected, they reported3.

The team also discovered an unidentified mutation that kills flies when combined with another mutation called chico. But with the parasite present, such flies survive. “Now you have a new mutant that should have killed the fly,” says Karr. “Wolbachia is keeping it alive.”

Finally, they studied the distribution of the bacteria in fly larvae, and found that every tissue was infected. “It raises the bar of the alarm,” says Karr. In theory this could affect any characteristic, he warns.

It looks as though no Drosophila geneticist can afford to ignore the possibility of Wolbachia influencing their results. “Now the paper is coming out, people are going to have to deal with it,” says Karr. “It remains an open question how much of an effect there will be.”

Other researchers say Karr's findings are important, but Drosophila genetics is not in any kind of crisis. “These are complicated animals,” says Gerry Rubin, a Drosophila geneticist based at the Howard Hughes Medical Institute near Washington DC. The idea that an environmental factor could be affecting the flies' biology is nothing new, he says. “It isn't the first thing we didn't know about and it won't be the last.” Knowing about these effects means researchers can now take them into account in experiments. “You can't control for things you don't know about,” Rubin says.

Kathy Matthews, co-director of the Bloomington collection, says there are no plans to ‘cure’ the infected stocks with antibiotics. “This paper will help alert Drosophila researchers to the possibility of the presence of Wolbachia in any given stock, which is certainly a good thing,” she told Nature. “But we view it more as a useful point of information about Drosophila biology than a call to arms.”

That's good news for Karr, who sees the infected strains as a tremendous resource for probing the interaction between Wolbachia and its host, and perhaps for learning about how parasites make the transition to becoming symbionts. He also hopes to undertake a systematic study of all the mutants at Bloomington, comparing cured with uncured lines to see how they are affected by the parasite.

References

  1. 1

    Starr, D. & Cline, T. Nature 418, 76–79 (2002).

  2. 2

    Driver, C., Georgiou, A. & Georgiou, G. Biogerontology 5, 185–192 (2004).

  3. 3

    Clark, M. E., Anderson, C., Cande, J. & Karr, T. L. Genetics doi:10.1534/genetics.104.038901 (2005).

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www.genetics.org

www.genetics.org/cgi/rapidpdf/genetics.104.038901v1

Drosophila stock centre at Indiana University

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Ainsworth, C. Parasite infiltrates fruitfly research. Nature 436, 8 (2005) doi:10.1038/436008a

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