A protein has been identified that enables cells to survive when dislodged from their substrate, and to migrate to new sites in the body. Such a mechanism might give cancer cells a significant advantage.
Usually, the cells of an organ stay close to home. In fact, their lives depend on it. Within their own neighbourhood, they communicate to mutual benefit with the cells around them, while signals from the matrix beneath tell them that they are on home ground1. When they lose contact with the matrix they die, in a process named ‘anoikis’ (from the Greek for ‘homelessness’)2. By contrast, cancer cells do not become fatally homesick if separated from home turf — quite the opposite. The ability to leave home and survive, even thrive, in a foreign tissue is essential for them to invade and metastasize. This strategy promotes the expansion and dissemination of the cancer-cell population, but sadly is often fatal to the patient. Now Douma and colleagues3 describe how, by screening for cells that can resist anoikis, they have identified a new protein player in metastasis (page 1034 of this issue).
Douma et al.3 studied rat intestinal epithelial cells — those that line the intestine. The malignant transformation of epithelial cells is described classically as the development of anchorage-independent growth4. To identify genes that enable cells to grow independently of anchorage to a matrix — that is, to survive the threat of anoikis — Douma et al. carried out an unbiased genetic screen. They delivered DNA molecules containing different genes into the rat cells in culture, and then transferred the cells from adhesive culture dishes into a non-adhesive environment.
Control cells died when separated from their matrix in this way. But one group of treated cells survived. These, the authors discovered, had received DNA molecules encoding the TrkB protein. TrkB is best known for its role in the nervous system, where, together with its main ligand, brain-derived neurotrophic factor (BDNF), it promotes the proliferation, differentiation and survival of normal neural components such as retinal cells and glial cells5. The epithelial cells described by Douma et al. co-opted this mechanism to overcome the threat of death through homelessness.
But the authors also found that the detached TrkB-expressing cells survived and continued to grow as spheroid aggregates. So could they have survived simply because they replaced adhesion to their substratum with adhesion to neighbouring cells? Apparently not. Further experiments by Douma et al. showed that, instead, TrkB production seemed to allow cells to resist anoikis by rewiring their internal circuitry. Specifically, TrkB triggered the activation of phosphatidylinositol-3-OH kinases (PI(3)Ks). These enzymes are known to stimulate another enzyme, the protein kinase AKT/PKB (ref. 6), and this too was active in the TrkB-producing cells. The result was a blockade of caspases, the DNA-cleaving enzymes that mediate anoikis and related forms of cell death.
PI(3)K enzymes also drive numerous other cellular functions associated with metastasis6,7,8. For instance, the PI(3)K pathway enables the cellular ‘skeleton’ to deform and ‘feet’ to protrude, thereby allowing cells to move — a key aspect of tissue invasion. The pathway is also implicated in stimulating the production of new blood vessels within tumours when oxygen levels are low. Not surprisingly, deregulation of the PI(3)K pathway has been reported in all the major types of epithelial cancer, and can be caused by the loss of upstream suppressor proteins, the constitutive activation of PI(3)Ks or the activation of downstream elements — and, it now seems, by the production of an upstream activator, TrkB. The involvement of this pathway may explain how TrkB by itself causes rat intestinal epithelial cells to be resistant to anoikis, and why, as Douma et al. show, this is sufficient to allow these cells to metastasize when injected into mice. In vivo, the inherent genetic instability of cancer cells might lead them to upregulate TrkB expression and activate downstream events.
There are several examples of molecules that are necessary, or even sufficient, to induce the full spectrum of characteristics necessary for metastasis in experimental models. A recent example is Twist, a gene-transcription factor that is better known for its regulation of changes in body shape during development9. We can speculate that the molecular programme required for metastasis can be triggered from a variety of starting points. But, given Douma et al.'s findings, we might also expect any process of metastasis induction to include a component that renders cells able to survive independently of attachment to the matrix or to other cells. In keeping with this expectation, forced expression of Twist stimulates cell invasion and causes the loss of cell–cell adhesion that is mediated through the E-cadherin protein9. Perhaps Twist can also co-opt the PI(3)K pathway and AKT/PKB, which are known to be involved in E-cadherin-mediated cell–cell recognition10.
Given that TrkB enables cells to survive when homeless, and thus to metastasize, might blocking this protein be an effective anticancer treatment? Perhaps, but we should be realistic about this expectation. Tumour cells can survive by means of an autostimulatory (autocrine) signalling loop, such as that mediated by TrkB and BDNF, or through a paracrine cross-communication with their environment (Fig. 1). Such cross-talk might use the same molecules: for example, BDNF is produced by the cells that line blood vessels, and production is enhanced by low oxygen conditions11; these events could promote the survival of both tumour cells and newly formed blood vessels, in the primary tumour or at a metastatic site. But there are other molecules involved in paracrine signalling, and we should be prepared for the possibility that strategies that target TrkB could be thwarted through these channels.
Homelessness is only one of a multitude of environmental stresses that can befall a cell. One way to overcome such problems is to seek a new and more salubrious location. Thus cells frequently need to migrate in order to survive. For instance, yeast that are poorly nourished extend migratory strands (hyphae) to seek better conditions. Similarly, slime-mould colonies that are deprived of nutrients send out scouts. And cancer cells, faced with a shortage of oxygen, hormones or nutrients, or with overcrowding, might survive by metastasizing — invading local tissue, entering the lymph and blood streams, exiting the circulation at a distant site, and, finally, establishing a secondary colony.
Avoiding death in a harsh or foreign environment seems to be a basic requirement for all types of nomadic behaviour. A mechanism such as that described by Douma et al.3, which promotes both survival and migration, would give tumour cells (and new blood vessels) a significant advantage. It might even be essential for tumour cells to pass the fitness test for metastasis.