News & Views | Published:

Computational biology

Biosensor design

A series of bacterial receptor proteins have been 'redesigned' by computer so that they bind molecules that are quite different from their natural ligands. The approach might be useful for designing catalytic proteins.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Looger, L. L., Dwyer, M. A., Smith, J. J. & Hellinga, H. W. Nature 423, 185–190 (2003).

  2. 2

    Hoess, R. Chem. Rev. 101, 3205–3218 (2001).

  3. 3

    Dower, W. J. & Mattheakis, L. C. Curr. Opin. Biotechnol. 6, 390–398 (2002).

  4. 4

    Tam, R. & Saier, M. H. Jr Microbiol. Rev. 57, 320–346 (1993).

  5. 5

    Desjarlais, J. R. & Clarke, N. D. Curr. Opin. Struct. Biol. 8, 471–475 (1998).

  6. 6

    Lasters, I., De Maeyer, M. & Desmet, J. Protein Eng. 8, 815–822 (1995).

  7. 7

    Dahiyat, B. I. & Mayo, S. L. Science 278, 82–87 (1997).

  8. 8

    Ghirlanda, G., Lear, J. D., Lombardi, A. & DeGrado, W. F. J. Mol. Biol. 281, 379–391 (1998).

  9. 9

    Reina, J. et al. Nature Struct. Biol. 9, 621–627 (2002).

  10. 10

    Wentworth, P. Jr & Janda, K. D. Cell Biochem. Biophys. 35, 63–87 (2001).

  11. 11

    Bolon, D. N. & Mayo, S. Proc. Natl Acad. Sci. USA 98, 14272–14279 (2001).

  12. 12

    Baltzer, L. & Nilsson, J. Curr. Opin. Biotechnol. 12, 355–360 (2001).

Download references

Author information

Correspondence to William F. DeGrado.

Rights and permissions

Reprints and Permissions

About this article

Further reading

Figure 1: Computational design of binding proteins.

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.