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Herbicides

Feminization of male frogs in the wild

Water-borne herbicide threatens amphibian populations in parts of the United States.

Abstract

Atrazine is the most commonly used herbicide in the United States and probably in the world1. Here we investigate the effects of exposure to water-borne atrazine contamination on wild leopard frogs (Rana pipiens) in different regions of the United States and find that 10–92% of males show gonadal abnormalities such as retarded development and hermaphroditism. These results are supported by laboratory observations, which together highlight concerns over the biological effects of environmental atrazine on amphibians.

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Figure 1: Use of the herbicide atrazine in the United States, on the basis of sales11.
Figure 2: Testicular oogenesis in wild leopard frogs.

References

  1. US Environmental Protection Agency. Federal Register 59, 60412–60443 (1994).

  2. Akingbemi, B. T. & Hardy, M. P. Ann. Med. 33, 391–403 (2001).

    Article  CAS  Google Scholar 

  3. Hayes, T. B. et al. Proc. Natl Acad. Sci. USA 99, 5476–5480 (2002).

    Article  ADS  CAS  Google Scholar 

  4. Tevera-Mendoza, L. et al. Environ. Toxicol. Chem. 21, 527–531 (2002).

    Article  Google Scholar 

  5. Reeder, A. L. et al. Environ. Health Perspect. 106, 261–266 (1998).

    Article  CAS  Google Scholar 

  6. Sanderson, J. T., Letcher, R. J., Heneweer, M., Giesy, J. P. & van den Berg, M. Environ. Health Perspect. 109, 1027–1031 (2001).

    Article  CAS  Google Scholar 

  7. Crain, D. A., Guillette, L. J. Jr, Rooney, A. A. & Pickford, D. B. Environ. Health Perspect. 105, 528–533 (1997).

    Article  CAS  Google Scholar 

  8. Sanderson, J. T., Seinen, W., Giesy, J. P. & van den Berg, M. Toxicol. Sci. 54, 121–127 (2000).

    Article  CAS  Google Scholar 

  9. Kimbrough, R. A. & Litke, D. W. Pesticides in Surface Water in Agricultural and Urban Areas of the South Platte River Basin from Denver, Colorado to North Platte, Nebraska, 1993–94 (NAWQA Program, South Platte River Basin Study, Denver, Colorado, 1995).

  10. Wake, D. B. Science 253, 860 (1991).

    Article  ADS  CAS  Google Scholar 

  11. Battaglin, W. A. & Goolsby, D. A. Water-Resources Invest. Rep. 94–4176 (US Geol. Surv., Denver, Colorado, 1995).

  12. Stebbins, R. A Field Guide to Western Reptiles and Amphibians: Field Marks of All Species in Western North America, Including Baja California (Houghton–Mifflin, Boston, 1985).

    Google Scholar 

  13. Conant, R. A Field Guide to Reptiles and Amphibians: Eastern and Central North America (Houghton–Mifflin, Boston, 1998).

    Google Scholar 

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Correspondence to Tyrone Hayes.

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Hayes, T., Haston, K., Tsui, M. et al. Feminization of male frogs in the wild. Nature 419, 895–896 (2002). https://doi.org/10.1038/419895a

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