Gene expression

RNA interference in adult mice

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RNA interference is an evolutionarily conserved surveillance mechanism that responds to double-stranded RNA by sequence-specific silencing of homologous genes. Here we show that transgene expression can be suppressed in adult mice by synthetic small interfering RNAs and by small-hairpin RNAs transcribed in vivo from DNA templates. We also show the therapeutic potential of this technique by demonstrating effective targeting of a sequence from hepatitis C virus by RNA interference in vivo.

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Figure 1: RNA interference in adult mice.


  1. 1

    Elbashir, S. M. et al. Nature 411, 494–498 (2001).

  2. 2

    Zhang, G., Budker, V. & Wolff, J. A. Hum. Gene Therapy 10, 1735–1737 (1999).

  3. 3

    Liu, F., Song, Y. & Liu, D. Gene Therapy 6, 1258–1266 (1999).

  4. 4

    Chang, J., Sigal, L. J., Lerro, A. & Taylor, J. J. Virol. 75, 3469–3473 (2001).

  5. 5

    Contag, C. H. et al. Photochem. Photobiol. 66, 523–531 (1997).

  6. 6

    Yant, S. R. et al. Nature Genet. 25, 35–41 (2000).

  7. 7

    Bitko, V. & Barik, S. BMC Microbiol. 1, 34 (2001).

  8. 8

    Paddison, P. J., Caudy, A. A., Bernstein, E., Hannon, G. J. & Conklin, D. S. Genes Dev. 16, 948–958 (2002).

  9. 9

    Tuschl, T. Nature Biotechnol. 20, 446–448 (2002).

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Correspondence to Mark A. Kay.

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The authors declare no competing financial interests.

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