The genetically controlled orientation of floral sex organs encourages cross-pollination.
Enantiostyly is a plant sexual polymorphism in which female sex organs are deflected to the left or right — resulting in 'mirror-image' flowers — but, although it occurs in at least a dozen unrelated families of flowering plants1 and has been known for over a century2, its adaptive significance has been unclear. Here we show that a mendelian locus governs the inheritance of style orientation and that this curious form of sexual asymmetry functions to promote cross-pollination in bee-pollinated plants.
To investigate whether enantiostyly is genetically determined, we carried out controlled crosses on Heteranthera multiflora, a species with left- and right-styled plants. Progeny ratios in families of the first and second generations (F1 and F2) indicated that style deflection is governed by a mendelian locus at which the right-styled allele (R) is dominant to left (r).
All crosses between genotypically homozygous plants with opposite style orientations (8 families, n = 242 progeny) produced only right-styled plants. All F2 segregations conformed to the expected 3:1 ratio of right- to left-styled plants (6 families, n = 242 progeny; right, 0.76; left, 0.24; deviation from 3:1, Ghet = 1.273, P = 0.93; Gpooled = 0.139, P = 0.71). The discovery of a mendelian locus that controls style orientation is, to our knowledge, the first demonstration of a gene for 'handedness' in plants.
With single-locus control of style orientation and equivalent levels of cross-pollination between left- and right-styled plants, a 1:1 ratio of the two style morphs should occur in natural populations of enantiostylous plants. This is the case in Wachendorfia paniculata populations3, suggesting that polymorphism in this species is also likely to be simply inherited.
Bees visiting enantiostylous flowers pick up pollen on the sides of their bodies, which favours transfer between flowers of opposite style deflection4 and suggests that enantiostyly promotes cross-pollination in a way that is functionally analogous to heterostylous sexual polymorphisms5. But although this makes sense for enantiostylous species in which plants have one or other stylar direction (dimorphic enantiostyly), species that produce both left- and right-styled flowers on an individual plant (monomorphic enantiostyly) are common and present a problem for the cross-pollination hypothesis1. This is because the presence of left- and right-styled flowers on the same plant could promote interfloral self-pollination, resulting in inbreeding depression and loss of outcross mating opportunities through pollen discounting6.
However, monomorphic enantiostyly probably evolved from an ancestor with straight styles. In straight-styled plants, all flowers on the plant can potentially donate and receive pollen from one another, whereas between-flower transfer should be relatively reduced in monomorphic enantiostyly (depending on the ratio of left- and right-styled flowers on a plant).
We investigated the role of enantiostyly in promoting cross-pollination in experimental arrays of Solanum rostratum (Fig. 1), a species with monomorphic enantiostyly that is visited primarily by bumble-bees4. By using marker genes and manipulating the flowers and inflorescences, we compared mating patterns in arrays composed of plants with the same number of flowers, but with each array having one of three stylar arrangements: straight styles, monomorphic enantiostyly or dimorphic enantiostyly (Fig. 2).
We predicted that outcrossing rates should be highest for arrays with dimorphic enantiostyly, lower for those with monomorphic enantiostyly, and lowest in those composed of straight-styled plants. For dimorphic enantiostyly, most mating should also occur between plants with opposite style orientations. Our results confirmed these predictions (Fig. 2).
We have shown that enantiostyly can increase the precision of cross-pollen transfer. The multiple origin of mirror-image flowers among angiosperm families provides a striking example of a convergent floral strategy that promotes pollen dispersal in bee-pollinated plants.
References
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Jesson, L., Barrett, S. Solving the puzzle of mirror-image flowers. Nature 417, 707 (2002). https://doi.org/10.1038/417707a
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DOI: https://doi.org/10.1038/417707a
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