Abstract
Hagfish and lampreys are unusual for modern vertebrates in that they have no jaws and their skeletons are neither calcified nor strengthened by collagen — the cartilaginous elements of their endoskeleton are composed of huge, clumped chondrocytes (cartilage cells). We have discovered that the cartilage in a 370-million-year-old jawless fish, Euphanerops longaevus, was extensively calcified, even though its cellular organization was similar to the non-mineralized type found in lampreys. The calcification of this early lamprey-type cartilage differs from that seen in modern jawed vertebrates, and may represent a parallel evolutionary move towards a mineralized endoskeleton.
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E. longaevus is an anaspid 'ostracoderm' (one of an ensemble of jawless fish aged 470–370 Myr) from the Late Devonian locality of Miguasha, Canada (Fig. 1). Although the endoskeleton of anaspids was previously thought not to have been calcified, that of large, recently discovered specimens of Euphanerops shows extensive calcification. All endoskeletal elements of these specimens show the same, foam-like aspect and are made up of minute, hollow, spherical bodies that are loosely attached either by their walls or by an intervening matrix (Fig. 2a). Thin sections reveal that these spherical bodies are sometimes divided into two chambers, and that their walls are composed of calcified cartilage with evidence of growth rings.
This structure is strikingly similar to that of lamprey cartilage, in which large chondrocyte cells, often in groups of two or four (cell 'nests'1), are surrounded by a 'territorial' matrix with concentric rings that give rise to differential staining (Fig. 2b). In lamprey cartilage that is artificially calcified in vitro, calcium phosphate is deposited first in the territorial matrix (black dots in Fig. 2c) and then in the intervening matrix2, generating the same pattern as is found in Euphanerops.
It is likely that the spherical bodies in Euphanerops contained chondrocytes, surrounded by calcified territorial matrix and held together loosely by a less-calcified intervening matrix. This process of calcification also occurs in the early stages of development of the higher jawed vertebrates (gnathostomes), but it must have occurred later in life in Euphanerops.
Ostracoderms are thought to be more closely related to gnathostomes than to either lampreys or hagfish3,4, but our discovery suggests that the cartilage of Euphanerops was structurally similar to that of lampreys, and that it may also have been non-collagenous. This does not necessarily imply a close relationship between anaspids and lampreys, as has been proposed5. There is no evidence that the calcification pattern that is found in Euphanerops, and which can be imposed in vitro on lamprey cartilage, is a precursor of the large-scale, globular calcification of cartilage and bone that is seen in more derived ostracoderms and in gnathostomes, although it may represent a parallel –– but less successful — move towards developing a calcified endoskeleton.
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Janvier, P., Arsenault, M. Calcification of early vertebrate cartilage. Nature 417, 609 (2002). https://doi.org/10.1038/417609a
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DOI: https://doi.org/10.1038/417609a
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