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Microbiology

Eukaryotic diversity in Spain's River of Fire

This ancient and hostile ecosystem hosts a surprising variety of microbial organisms.

Abstract

The Rio Tinto, known by the Phoenicians as 'Ur-yero', or 'River of Fire', because of its deep red colour and high acidity, flows through the world's largest pyritic belt in southwestern Spain. Surprisingly, eukaryotic microbes are the principal contributors of biomass in this hostile river, which has a pH of 2 and contains much higher concentrations of heavy metals than are typically found in fresh waters. Here we show that the Rio Tinto shows an unexpected degree of eukaryotic diversity and includes new lineages that we have identified by sequence analysis of genes encoding small-subunit ribosomal RNAs. The diversity of these eukaryotes is much greater than that of prokaryotes, whose metabolism is responsible for the extreme environment.

Main

The Rio Tinto (Fig. 1) is 90 km long. Unlike other extreme acidic environments, the low pH and high concentrations of heavy metals in this vast ecosystem (3–20 g iron per litre of water, for example) predate 5,000 years of human mining activity, with iron-oxidizing bacteria having existed in the river basin some 300,000 years ago1.

Figure 1: The Rio Tinto at La Palma del Condado, Spain.
figure 1

J. L. DE LOPE & J. M. SANCHEZ

The river's red colour is due to the high concentration of iron dissolved in its extremely acidic waters.

Morphology-based investigations first alerted us to the possible variety of eukaryotic lineages in the Rio Tinto. Our molecular analysis reveals the extent of this eukaryotic diversity — which is quite unexpected in such an acidic and highly metal-rich environment — as well as several taxa and cosmopolitan organisms that have never previously been reported in extreme habitats (Fig. 2).

Figure 2: Phylogeny based on minimum-evolution analyses of ribosomal RNAs using a likelihood model.
figure 2

Bold letters indicate environmental clones. RT, sequences from Rio Tinto species; cul, sequences from cultured species. Underlined taxa represent genera that have been identified in the river by microscopy. Sampling sites: RT1, La Palma; RT3, Berrocal Upper; RT5i, the Origin, black filamentous biofilm; RT5ii, the Origin, green filamentous biofilm; RT7i, Anabel's Garden, green biofilm; RT7ii, Anabel's Garden, yellow biofilm. Bootstrap values are shown next to branches. Sequences have been deposited at GenBank under accession numbers AY082969–AY083001.

Eukaryotic algae contribute at least 60% of the biomass in the Rio Tinto2, forming conspicuous patches on the river's surface. Our molecular analysis detected many further, non-photosynthetic lineages, such as ciliates, cercomonads, vahlkampfiid amoebae, stramenopiles and fungi, which have all previously escaped detection3.

The phylogenetic placement of several sequences was consistent with the idea that they represent new eukaryotic lineages. Two groups of clones branched as possible sister taxa to amoeboid lineages4,5: the first of these (clones RT5iin14 and RT5iin16) branched at the base of the animal–fungal–nucleariid radiation; the second (RT5iin21 and RT5iin44) branched with the filose amoeba (Filamoeba nolandi), with moderately strong bootstrap support (83%). However, the long-branched nature of these sequences makes it difficult to position them precisely in a large phylogeny. The clone RT5iin25 may represent a new stramenopile lineage, with only weak bootstrap values supporting its relationship to Cafeteria roenbergensis.

The evolutionary distances between acidophilic species that occur in the river and their neutrophilic relatives offer insight into adaptation to an extremely acidic environment. We detected clones from the Rio Tinto that are closely related to neutrophilic species such as Chlamydomonas noctigama, Chlorella minutissima and Colpidium campylum. Adaptations associated with the transition from a neutral to an acidic environment must occur relatively rapidly when measured on evolutionary timescales.

We know from microscopic observations that rotifers also inhabit the river, as do heliozoa and other types of amoeba. The wide eukaryotic diversity associated with low pH and high concentrations of heavy metals indicates that adaptation to such adverse conditions must be more easily accomplished than was previously thought. As eukaryotes rarely flourish in extreme environments, our results challenge former ideas about the phylogenetic range of organisms that are capable of living in extreme environments.

References

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Correspondence to Mitchell L. Sogin.

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Amaral Zettler, L., Gómez, F., Zettler, E. et al. Eukaryotic diversity in Spain's River of Fire. Nature 417, 137 (2002). https://doi.org/10.1038/417137a

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