DISC1 is involved in neurite outgrowth through its binding to FEZ1. (a) Interaction between DISC1 and FEZ1 was upregulated during neurite outgrowth. Stably human DISC1-FLAG-expressing PC12 cells were treated with NGF (50 ng/ml) for 24 h and collected. Lysates were immunoprecipitated by anti-FLAG antibody. Immunoprecipitated complexes were subjected to Western blot analysis using anti-FEZ1 antibody. (b–m) Stably DISC1-expressing PC12 cells (h–j and k–m showing line #1 and #2, respectively) exhibited enhanced extension of neurites upon the stimulation with NGF compared to mock-stable cells (b–d and e–g showing line #1 and #2, respectively). Cells were treated with NGF (50 ng/ml) for 0 h (b, e, h, k), 24 h (c, f, i, l), and 48 r (d, g, j, m) and their neurites were microscopically observed.