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  • Original Research Article
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Trapping and sequence analysis of 1138 putative exons from human chromosome 18

Molecular Psychiatry volume 8, pages 619–623 (2003)Cite this article

Abstract

In a search for novel genes on chromosome 18 (HC18), on which several regions have been linked to bipolar disorder, we applied exon trapping to HC18-specific cosmids. Among the 1138 exons trapped, 1052 of them have been mapped to HC18, and the remaining 86 have not been localized. No exons were localized to genomic regions other than HC18. BLAST database search revealed that 190 exons were identical to 98 Unigenes on HC18; 98 identical to additional 82 clusters of ESTs not present in the HC18 Unigene set; 39 homologous to genes from human and other species (e<10−3); and the remaining 811 exons had no significant homology to transcripts in public databases. The mapped exons were compared to the 867 annotated genes on HC18 in the Celera databases; 216 exons were identical to 104 Celera ‘genes’ and the remaining 836 exons were not found in the Celera databases. On average, there were two exons for a matched transcript (known genes and ESTs). Therefore, the 850 novel exons may represent hundreds of novel genes on chromosome 18.

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Acknowledgements

This study is supported by grants from the Theodore and Vada Stanley Foundation (CAR, MGM, and JRD), and grants from NARSAD, National Alliance for Research on Schizophrenia and Depression (MGM, HC, JRD). We thank Jillian Cooper for the COS-7 cell line. We are grateful to Dr Paul McHugh and the Department of Psychiatry for the support of this work. The laboratory of SEA is supported by grants from the Swiss Science National Foundation and the European Union.

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Authors and Affiliations

  1. Department of Psychiatry and Behavioral Sciences, Johns Hopkins University School of Medicine, Baltimore, USA

    H Chen, N Wang, Y Huo, D F MacKinnon, J B Potash, J Raymond DePaulo Jr, C A Ross & M G McInnis

  2. Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, USA

    C A Ross

  3. Massachusetts General Hospital, Boston, USA

    P Sklar

  4. National Institute of Mental Health, National Institutes of Health Bethesda, USA

    F J McMahon

  5. Division of Medical Genetics, University of Geneva Medical School, Geneva, Switzerland

    S E Antonarakis

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  1. H Chen
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  2. N Wang
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  3. Y Huo
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  4. P Sklar
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  9. J Raymond DePaulo Jr
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  10. C A Ross
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  11. M G McInnis
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Corresponding authors

Correspondence to H Chen or M G McInnis.

Additional information

The sequences reported here have GenBank nos BH608634-BH609385, BH614713-BH614791, and AF149426-AF149699. The LL18NC02 cosmid library was constructed in the Lawrence Livermore National Laboratory, and distributed by the UK HGMP Resource Center.

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Chen, H., Wang, N., Huo, Y. et al. Trapping and sequence analysis of 1138 putative exons from human chromosome 18. Mol Psychiatry 8, 619–623 (2003). https://doi.org/10.1038/sj.mp.4001288

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  • DOI: https://doi.org/10.1038/sj.mp.4001288

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