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Mutation screening and LD mapping in the VCFS deleted region of chromosome 22q11 in schizophrenia using a novel DNA pooling approach

Molecular Psychiatry volume 7, pages 1092–1100 (2002)Cite this article

Abstract

We examined whether variation within six genes from the VCFS critical region at 22q11 (DGSC, Stk22A1, DGSI, Gscl, Slc25A1 and Znf74) confers susceptibility to schizophrenia. We screened the exons and flanking intronic sequence of each gene for mutations in 14 individuals with DSM-IV schizophrenia using DHPLC. All polymorphisms identified were characterised and genotyped in a sample of 184 schizophrenics and matched controls, using novel DNA pooling methods. Of the polymorphisms identified, 17 were located within exons, six were within coding sequence, and two were non-synonymous. Pooled genotyping revealed no differences in the allele frequencies for any polymorphism between cases and controls that met our pre-defined criterion (P ⩽ 0.1). In a complementary approach we also attempted to define the location of a schizophrenia susceptibility locus more precisely by performing association mapping using seven microsatellites spanning the VCFS region with an average inter-marker distance of 450 kb. Conventional χ2 analysis of genotypes in 368 cases and 368 controls revealed that none of the markers was significantly associated (P < 0.05) with schizophrenia. However, evidence for significant association (P = 0.003) was obtained for D22S944 when alleles were combined. TDT analysis of D22S944 genotyped in a further 278 cases of schizophrenia and their parents failed to find any overall allele-wise significant transmission disequilibrium (χ2 = 18.3, P = 0.17). However, individual analysis of the alleles revealed that allele 12 was excessively non-transmitted and that this almost reached significance when corrected for multiple alleles (χ2 = 7.35, P = 0.006, P = 0.078 corrected for 13 alleles).

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Acknowledgements

This work was supported by the MRC (UK). The collection of families in Bulgaria is sponsored by the Janssen Research Foundation. We thank David Collier for supplying us with allele frequency data for D22S944.

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Authors and Affiliations

  1. Department of Psychological Medicine, University of Wales College of Medicine, Cardiff, UK

    N M Williams, G Spurlock, N Norton, H J Williams, M L Hamshere, M Krawczak, G Kirov, S Jones, A G Cardno, M C O'Donovan & M J Owen

  2. Medical University Sofia, Medical Genetics, Sofia, Bulgaria

    I Nikolov & L Georgieva

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Correspondence to H J Williams.

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Williams, N., Spurlock, G., Norton, N. et al. Mutation screening and LD mapping in the VCFS deleted region of chromosome 22q11 in schizophrenia using a novel DNA pooling approach. Mol Psychiatry 7, 1092–1100 (2002). https://doi.org/10.1038/sj.mp.4001188

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