Control of compartmental affinity boundaries by Hedgehog

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Abstract

In Drosophila, each segmental primordium is subdivided into two cell populations, the anterior (A) and posterior (P) compartments by the selective activity of the transcription factor Engrailed (En) in P cells1,2,3,4. Under En control, P cells secrete, but cannot respond to, the signalling protein Hedgehog (Hh)5,6,7. In contrast, and by default, A cells are programmed to respond to Hh by expressing other signalling molecules, such as Decapentaplegic (Dpp) and Wingless (Wg), which organize growth and patterning in both compartments5,7,8,9. Cells of the A and P compartments do not intermix, apparently as a consequence of their having distinct cell affinities that cause them to maximize contact with cells of the same compartment, while minimizing contact with cells from the other compartment10. This failure to mix has previously been ascribed to an autonomous and direct role for En in specifying a P, as opposed to an A, cell affinity3,11,12,13. However, an alternative hypothesis is that Hh secreted by P cells induces A cells to acquire a distinct cell affinity, ensuring that a stable ‘affinity boundary’ forms wherever P and A cells meet. Here we show that the affinity boundary that segregates A and P cells into adjacent but immiscible cell populations is to a large extent a consequence of local Hh signalling, rather than a reflection of an intrinsic affinity difference between A and P cells.

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Figure 1: Smo cells redirect the A/P affinity boundary.
Figure 2: Smo cells that crossed the affinity boundary autonomously form ectopic A pattern.
Figure 3: Constitutive activation of the Hh signal-transduction pathway can rescue the abnormal segregation behaviour of smo cells and cause smo +A cells to minimize contact with surrounding cells that do not receive the Hh signal.

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Acknowledgements

We thank J. Nüsslein-Volhard, Y. Chen and G. Struhl for fly stocks; R. Burke, C.Dahmann, N. Méthot and particularly G. Struhl for comments on the manuscript; F. Cifuentes, D.Nellen and M. Zecca for help with the clonal analysis; and J. Modolell for support. I.R. was the recipient of an EMBO fellowship. This work was supported by the Swiss National Science Foundation, the Direccion general de Investigacion Cientifica y Tecnica, and the Fundacion Ramon Areces.

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Correspondence to Konrad Basler.

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Rodriguez, I., Basler, K. Control of compartmental affinity boundaries by Hedgehog. Nature 389, 614–618 (1997) doi:10.1038/39343

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