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TGF-β1 neutralizing antibodies decrease the fibrotic effects of ischemic priapism

Abstract

The objective of this study was to evaluate the possible role of transforming growth factor beta 1 (TGF-β1) antibodies (ab) for the prevention of fibrotic effects of priapism in a rat model. In total, 30 adult Sprague–Dawley rats were divided into five groups. Priapism with 6 h (group 1), priapism with 6 h+ab (group 2), priapism with 24 h (group 3), priapism with 24 h+ab (group 4) and control (group 5). Priapism was induced with a vacuum erection device and a rubber band was placed at the base of the erect penis. At 1 h after the initiation of priapism, TGF-β1 antibodies were given intracavernosaly. All rats underwent electrical stimulation of the cavernous nerve after 8 weeks. Intracavernous and systemic blood pressures were measured during the procedure. Rats in group 1 showed significantly higher (intracavernosal pressure (ICP) pressures to cavernous nerve stimulation and had higher ICP/BP ratios when compared to other groups. Similarly, histopathologic examination revealed less fibrosis in group 2, compared with the other groups. Consequently, TGF-β1 antibodies antagonise the fibrotic effects of TGF-β1, especially in cases with duration of priapism less than 6 h.

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Correspondence to A Kadioglu.

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Source of funding: Istanbul University Research Foundation.

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Sanli, O., Armagan, A., Kandirali, E. et al. TGF-β1 neutralizing antibodies decrease the fibrotic effects of ischemic priapism. Int J Impot Res 16, 492–497 (2004). https://doi.org/10.1038/sj.ijir.3901261

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  • DOI: https://doi.org/10.1038/sj.ijir.3901261

Keywords

  • priapism of penis and clitoris
  • animal models

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