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Selegiline enhances erectile activity induced by dopamine injection in the paraventricular nucleus of the hypothalamus in anesthetized rats

Abstract

Apomorphine delivered in the paraventricular nucleus of the hypothalamus (PVN) induces penile erection in rats, suggesting a role of dopaminergic projection to the PVN in the control of penile erection. We assessed whether the selective inhibitor of monoamine oxidase B, selegiline, could enhance the erectile activity induced by dopamine delivery in the PVN. Intracavernous and blood pressure (ICP and BP) were monitored in anesthetized rats to quantify ICP rises (number and percentage of ICP maximum/mean BP (ICPmax/BP×100)) elicited by 10 µg dopamine injection in the PVN after saline or 3 mg/kg i.v. selegiline (8 rats per group). The number of ICP rises (mean±s.d.: 4.5±2.9 vs 1.4±1.9; P=0.017) and their ICPmax/BP×100 (49±8% vs 34±9%; P=0.015) were significantly greater upon dopamine injection in the PVN than upon vehicle. Compared to saline i.v., 3 mg/kg selegiline pretreatment significantly increased the number of ICP rises induced by dopamine injection in the PVN (9.4±2.6 vs 4.5±2.9; P<0.001), without affecting their amplitude. This suggests that drugs potentiating dopaminergic responses in the central nervous system might enhance proerectile commands of supraspinal origin.

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References

  1. Melis MR, Argiolas A . Dopamine and sexual behavior Neurosci Biobehav Rev 1995; 19: 19–38.

    Article  CAS  Google Scholar 

  2. Melis MR, Argiolas A, Gessa GL . Apomorphine-induced penile erection and yawning: site of action in brain Brain Res 1987; 415: 98–104.

    Article  CAS  Google Scholar 

  3. Chen KK, Chan JY, Chang LS . Dopaminergic neurotransmission at the paraventricular nucleus of hypothalamus in central regulation of penile erection in the rat J Urol 1999; 162: 237–242.

    Article  CAS  Google Scholar 

  4. Heinonen EH, Myllyla V . Safety of selegiline (deprenyl) in the treatment of Parkinson's disease Drug Saf 1998; 19: 11–22.

    Article  CAS  Google Scholar 

  5. Paterson IA, Juorio AV, Berry MD, Zhu MY . Inhibition of monoamine oxidase-B by (–)-deprenyl potentiates neuronal responses to dopamine agonists but does not inhibit dopamine catabolism in the rat striatum J Pharmacol Exp Ther 1991; 258: 1019–1026.

    CAS  PubMed  Google Scholar 

  6. Heikkila RE, Cabbat FS, Manzino L, Duvoisin RC . Potentiation by deprenil of L-dopa induced circling in nigral-lesioned rats Pharmacol Biochem Behav 1981; 15: 75–79.

    Article  CAS  Google Scholar 

  7. Kato T, Dong B, Ishii K, Kinemuchi H . Brain dialysis: in vivo metabolism of dopamine and serotonin by monoamine oxidase A but not B in the striatum of unrestrained rats J Neurochem 1986; 46: 1277–1282.

    Article  CAS  Google Scholar 

  8. Butcher SP, Fairbrother IS, Kelly JS, Arbuthnott GW . Effects of selective monoamine oxidase inhibitors on the in vivo release and metabolism of dopamine in the rat striatum J Neurochem 1990; 55: 981–988.

    Article  CAS  Google Scholar 

  9. Wachtel SR, Abercrombie ED . L-3,4-dihydroxyphenylalanine-induced dopamine release in the striatum of intact and 6-hydroxydopamine-treated rats: differential effects of monoamine oxidase A and B inhibitors J Neurochem 1994; 63: 108–117.

    Article  CAS  Google Scholar 

  10. Oreland L, Arai Y, Stenstrom A . The effect of deprenyl (selegiline) on intra- and extraneuronal dopamine oxidation Acta Neurol Scand 1983; 95: 81–85.

    Article  CAS  Google Scholar 

  11. Okuda C, Segal DS, Kuczenski R . Deprenyl alters behavior and caudate dopamine through an amphetamine-like action Pharmacol Biochem Behav 1992; 43: 1075–1080.

    Article  CAS  Google Scholar 

  12. Heinonen EH, Anttila MI, Lammintausta RA . Pharmacokinetic aspects of L-deprenyl (selegiline) and its metabolites Clin Pharmacol Ther 1994; 56: 742–749.

    Article  CAS  Google Scholar 

  13. Yang HY, Neff NH . Beta-phenylethylamine: a specific substrate for type B monoamine oxidase of brain J Pharmacol Exp Ther 1973; 187: 365–371.

    CAS  PubMed  Google Scholar 

  14. Berry MD et al. The effects of administration of monoamine oxidase-B inhibitors on rat striatal neurone responses to dopamine Br J Pharmacol 1994; 113: 1159–1166.

    Article  CAS  Google Scholar 

  15. Nakamura M, Ishii A, Nakahara D . Characterization of beta-phenylethylamine-induced monoamine release in rat nucleus accumbens: a microdialysis study Eur J Pharmacol 1998; 349: 163–169.

    Article  CAS  Google Scholar 

  16. Mantle TJ, Houslay MD, Garrett NJ, Tipton KF . 5-Hydroxytryptamine is a substrate for both species of monoamine oxidase in beef heart mitochondria J Pharm Pharmacol 1976; 28: 667–671.

    Article  CAS  Google Scholar 

  17. Weiner N, Molinoff PB . Catecholamines In: Siegel G, Agranoff B, Albers RW, Molinoff P, (eds.) Basic Neurochemistry 4th Edn Raven Press: New York 1989 pp 233–251.

    Google Scholar 

  18. Knoll J, Yen TT, Miklya I . Sexually low performing male rats die earlier than their high performing peers and (–)deprenyl treatment eliminates this difference Life Sci 1994; 54: 1047–1057.

    Article  CAS  Google Scholar 

  19. Knoll J, Yen TT, Dallo J . Long-lasting, true aphrodisiac effect of (–)-deprenyl in sexually sluggish old male rats Mod Probl Pharmacopsychiatry 1983; 19: 135–153.

    Article  CAS  Google Scholar 

  20. Dallo J, Lekka N, Knoll J . The ejaculatory behavior of sexually sluggish male rats treated with (–)deprenyl, apomorphine, bromocriptine and amphetamine Pol J Pharmacol Pharm 1986; 38: 251–255.

    CAS  PubMed  Google Scholar 

  21. Ferrari F, Giuliani D . Inability of (–)deprenyl to modify copulatory performance in the male rat, whether or not stimulated by the selective D2 dopamine agonist SND 919 Pharmacol Res 1994; 29: 373–382.

    Article  CAS  Google Scholar 

  22. Heaton JP . Apomorphine: an update of clinical trial results Int J Impot Res 2000; 12: (Suppl 4) S67–S73.

    Article  Google Scholar 

Download references

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Correspondence to F Giuliano.

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Allard, J., Bernabe, J., Derdinger, F. et al. Selegiline enhances erectile activity induced by dopamine injection in the paraventricular nucleus of the hypothalamus in anesthetized rats. Int J Impot Res 14, 518–522 (2002). https://doi.org/10.1038/sj.ijir.3900934

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Keywords

  • penile erection
  • monoamine oxidase
  • l-deprenyl
  • dopamine

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