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Comparison of effects of angiotensin peptides in the regulation of clitoral cavernosum smooth muscle tone

International Journal of Impotence Research volume 14, pages 72–80 (2002)Cite this article

Abstract

The isometric tension measurement and in vitro autoradiography were used in clitoral cavernosum smooth muscle (CSM). Angiotensin ANG III, ANG IV, ANG II and ANG I induced contractions in clitoral CSM strips. ANG III and ANG I- induced contraction was five times less active than ANG II, whereas ANG IV-induced contraction was 1181-fold less potent than ANG II. Contractile responses to ANG III, ANG IV, ANG II and ANG I were significantly inhibited by type 1 ANG II (AT 1) receptor antagonist Dup 753 but not by type 2 ANG II (AT2) receptor antagonist PD 123,319. Pre-treatment with Nω-nitro-L-arginine methyl ester, nitric oxide (NO) synthase inhibitor accentuated force of contraction induced by ANG III, ANG IV and ANG II. Amastatin, an aminopeptidase inhibitor enhanced ANG III- and ANG IV-induced contractions. Specific binding sites for 125I-ANG II were found in the clitoral CSM. Specific binding of 125I-ANG II was displaced by unlabeled ANG peptides. This study suggests that the contractile responses to all four peptides of the ANG family are mediated via AT1 receptors but not AT2 receptors. Further, the rank order of potency of contraction was as follows, ANG II> ANG I>ANG III>ANG IV. It is also suggested that peptides of the ANG family have a cross-talk with the NO system and aminopeptidase is involved in the modulation of the tone of clitoral CSM by ANG III and ANG IV.

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Acknowledgements

This study was supported by grants from the Korea Health 21 Research and Development Project, Ministry of Health and Welfare, Republic of Korea (HMP-00-B-21400-0053), Korea Research Foundation (2000-041-F00239) and Alumni Association of Chonbuk National University Medical School.

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Authors and Affiliations

  1. Department of Urology, Chonbuk National University Medical School and Chonbuk National University Institute for Medical Sciences and Research Institute of Clinical Medicine of Chonbuk National University Hospital, Chonju, South Korea

    J K Park, J U Kim & Y G Kim

  2. Department of Physiology, Chonbuk National University Medical School and Chonbuk National University Institute for Medical Sciences and Research Institute of Clinical Medicine of Chonbuk National University Hospital, Chonju, South Korea

    S Z Kim, S M Kim & K W Cho

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  1. J K Park
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Correspondence to J K Park.

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Park, J., Kim, S., Kim, J. et al. Comparison of effects of angiotensin peptides in the regulation of clitoral cavernosum smooth muscle tone. Int J Impot Res 14, 72–80 (2002). https://doi.org/10.1038/sj.ijir.3900824

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