Abstract
Cytokine release from inflammatory cells, endotoxin, lipid peroxidation, and generation of reactive oxygen species are among the factors currently thought to be important in the pathogenesis of alcoholic and nonalcoholic steatohepatitis (SH). To more fully evaluate the role of mononuclear inflammatory cells in SH, 11 needle liver biopsies showing SH were selected for immunohistochemical staining to analyze the type and distribution of mononuclear inflammatory cells, including T and B lymphocytes and Kupffer cells (using immunostains for CD3, CD4, CD8; CD20; and CD68, respectively). An additional seven biopsies showing normal or fatty liver were also selected for CD68 immunostaining. Immunohistochemistry showed mild to moderate (1+ to 2+) numbers of T cells, with equal representation of CD4 and CD8 cells. T cells were found in portal tracts and in regions of SH. B cells were only rarely present. CD68 staining of simple fatty liver and normal liver showed elongated, spindle-shaped Kupffer cells diffusely distributed along the sinusoids throughout the lobules. In contrast, in cases of SH, there was prominent enlargement and aggregation of Kupffer cells in perivenular regions. Scattered large vacuoles of fat that had appeared to be within hepatocytes on routine stain were found actually to be within Kupffer cells. These results support the concept that hepatic Kupffer cells are a major immune effector cell in the pathogenesis of steatohepatitis. A potential direct Kupffer cell role in hepatic lipid processing is also postulated.
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INTRODUCTION
Steatohepatitis (SH) has received exceptional attention in clinical and research hepatology during the past decade because of the wide prevalence of alcohol use, obesity, and diabetes (1, 2). The pathogenesis of SH is related to the oxidative stress and lipid peroxidation associated with the fatty liver (3), endotoxemia (4), activation of hepatic stellate cells, and a complex cytokine network in which production of tumor necrosis factor (TNF)-α is prominent (5, 6, 7). Although classic morphologic studies of alcoholic steatohepatitis (ASH; 8, 9, 10) and, more recently, of nonalcoholic steatohepatitis (NASH; 11, 12, 13) have demonstrated the prominence of neutrophilic leukocytes within the centrilobular lesion of steatohepatitis (hepatocyte ballooning, fatty change, Mallory bodies, and pericellular/perisinusoidal fibrosis), current hypotheses concerning the pathogenesis of SH indicate major roles for lymphocytes and macrophages (Kupffer cells).
In this study, immunohistochemical stains of 11 needle liver biopsies showing steatohepatitis were used to demonstrate T cells (including CD4 and CD8 cells), B cells, and Kupffer cells. The type and distribution of these mononuclear cells were evaluated with regard to current data concerning the pathogenesis of SH.
MATERIALS AND METHODS
The files of the Division of Surgical Pathology of the Department of Pathology, Columbia-Presbyterian Medical Center, were searched for liver biopsies with a diagnosis of SH from 1990 to 2001. Eleven cases (needle biopsies) were selected, and the medical records of the respective patients were reviewed for history of alcohol use, obesity, diabetes or other putative etiologic factors. Seven control cases were also selected for CD68 (Kupffer cell) immunohistochemical staining once the CD68 staining results for SH cases were apparent. These included three normal liver biopsies, two with mild or moderate fat, and two with marked fat.
Immunohistochemical staining was performed on deparaffinized sections from paraffin-embedded, formalin-fixed needle liver biopsy specimens using the DAKO EnVision™ + System, Peroxidase (3,3′-diaminobenzidine), a two-step immunohistochemical technique using an avidin and biotin-free horseradish peroxidase–labeled polymer conjugated with secondary antibodies to bind to the primary antibody. The primary mouse monoclonal antibodies used included CD3 (T cells), CD8 (T cytotoxic/suppressor cells), CD20 (B cells), and CD68 (Kupffer cells/macrophages) from DAKO (Carpinteria, CA) and CD4 (T helper/inducer cells) from Novocastra, distributed by Vector (Burlingame, CA).
Immunohistochemical staining results were reviewed, and the numbers of respective positively stained immune cells were graded as follows: 0 (none); R (rare cells present); 1+ (few); 2+ (moderate numbers); 3 (many). Notation was made of their specific location, including perivenular (V), within portal tracts (P), within lobular sinusoids (L), and within fibrous septa (SE).
RESULTS
Immune cells in Steatohepatitis Cases
T and B cells
CD3-positive T cells were found in mild to moderate numbers within portal tracts and fibrous septa and in regions of perivenular steatohepatitis. The T cells were composed of an equal admixture of CD4- and CD8-positive cells. B cells were only rarely identified at any site.
Kupffer cells
Aggregates of prominent CD68-positive Kupffer cells were present in perivenular regions of SH, readily seen even at low magnification (Fig. 1A–C). At these sites they were characterized by enlarged and rounded cell bodies containing numerous densely stained intracellular granules (positive lysosomal staining). Elsewhere, along sinusoids in the midzonal and periportal regions, Kupffer cells were nonaggregated, individual cells with elongated tapering cell bodies identical to the appearance of CD68-stained Kupffer cells in control sections of normal liver (Fig. 2).
One case of NASH (Case 4) was sectioned serially and stained alternately with hematoxylin and eosin (H&E) and CD68 immunostaining to closely compare the routine light-microscopic appearances with the corresponding adjacent immunostain results (Fig. 3A and B). This case showed aggregated and enlarged Kupffer cells in sinusoids directly surrounding hepatocytes with severe hydropic swelling and Mallory bodies. Inspection of the H&E section disclosed the relative inconspicuousness of the Kupffer cells in these foci as well as the potential for misidentifying them as lymphocytes or endothelial cells. In addition, these sections demonstrated that some of the large lipid vacuoles that appeared to be within hepatocytes on routine H&E were within Kupffer cells (Fig. 3B). This was also found in other SH cases and in the control fatty livers (see below).
CD68 Immunostaining of Normal and Fatty Liver
The normal liver showed a diffuse sinusoidal distribution of elongated individual Kupffer cells (Fig. 2). This pattern was maintained in the fatty livers (Fig. 4A and B), without the perivenular aggregation seen in cases of SH. Kupffer cells in the fatty livers were occasionally enlarged (Fig. 4B) and rarely contained lipid vacuoles.
DISCUSSION
Although classic morphologic studies have long emphasized infiltration of neutrophils in the hepatic lesion of alcoholic hepatitis (8, 10), more recent investigations of the pathogenesis of SH in alcoholic and nonalcoholic individuals have demonstrated the presence of a complex cytokine network in which Kupffer cells and lymphocytes play important roles (5, 14). Among the cytokines involved in SH, generation of TNF-α and TNF-α–inducible cytokines such as interleukin-1, -6, and -8 were stressed in a recent overview of the topic by Tilg and Diehl (5). The significance of TNF-α activation of nuclear factor-kappa B in monocytes in alcoholics and the subsequent transcription of inflammation-related genes have also been the subject of recent studies (15, 16).
Our study reconfirms earlier work that immunohistochemically demonstrated T cells in the livers of individuals with alcoholic liver disease (14). A comparative light-microscopic study of ASH and NASH also recorded the presence of 1+ portal inflammation in 49 and 57% of cases, respectively (12), whereas another study of NASH found 21 of 49 cases to have predominance of perivenular mononuclear cells (13). In our study, portal tract and sinusoidal T cells were generally mild to moderate in number. B cells were rarely identified, as also shown in the study of Chedid et al. (14).
The most distinctive finding in our study was the presence of aggregates of enlarged Kupffer cells in perivenular regions of steatohepatitis, as compared with the diffuse, panlobular sinusoidal distribution seen in the normal or fatty liver without SH. On routine light microscopy, these Kupffer cells may be relatively inapparent or mistaken for lymphocytes or endothelial cells. The presence of Kupffer cells among the constituent inflammatory infiltrates of SH was not assessed in several classic pathologic studies of alcoholic hepatitis (8, 9, 10) but was cited in a recent ultrastructural and immunohistochemical investigation of stellate and Kupffer cells in 35 patients with alcoholic liver disease (17). The latter study also demonstrated clusters of Kupffer cells with CD68 immunostaining.
The finding of lipid vacuoles in some Kupffer cells suggests that these cells may be processing lipid or phagocytosing effete fatty hepatocytes. Lysosomal activity in these Kupffer cells could represent one mechanism by which reactive oxygen species are generated in the fatty liver.
The localization of Kupffer cells to perivenular regions of steatohepatitis is consonant with the current view that the pathogenesis of this condition is related to intrahepatic release of TNF-α by Kupffer cells, with subsequent generation of cytokines such as interleukin-1, interleukin-6, interleukin-8 and transforming growth factor-β (5). Induction of CD14 receptors on Kupffer cells by ethanol or by lipopolysaccharide of endotoxin appears to be a critical event preceding the cytokine cascade and production of reactive oxygen species by Kupffer cells (6, 7). Future immunohistochemical studies to evaluate expression of CD14 receptors on Kupffer cells in SH would provide additional support for this concept.
In summary, this study demonstrated the presence of T cells and Kupffer cells as constituents of the inflammatory lesion of ASH and NASH, with unusual prominence of enlarged and aggregated Kupffer cells present in perivenular regions of steatohepatitis. No significant differences between steatohepatitis of alcoholic versus nonalcoholic type were detected in the numbers or distribution of these inflammatory cells. Although previous histopathological criteria for the diagnosis of steatohepatitis have stressed the presence of neutrophil infiltrates, a contemporary comprehensive morphologic definition of this lesion should provide a more inclusive approach to the spectrum of inflammation seen, with particular recognition of the participation of Kupffer cells.
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Acknowledgements
The authors thank Dianne Alexis and Ritchie Alsberry for immunohistochemical staining and Alfred Lamme for photography.
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Lefkowitch, J., Haythe, J. & Regent, N. Kupffer Cell Aggregation and Perivenular Distribution in Steatohepatitis. Mod Pathol 15, 699–704 (2002). https://doi.org/10.1097/01.MP.0000019579.30842.96
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DOI: https://doi.org/10.1097/01.MP.0000019579.30842.96
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