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Stress-signalling kinase Sek1 protects thymocytes from apoptosis mediated by CD95 and CD3

Abstract

Distinct and evolutionarily conserved signal transduction cascades mediate survival or death in response to developmental and environmental cues. The stress-activated protein kinases, or Jun N-terminal kinases (SAPKs/JNKs)1,2, are activated in response to a variety of cellular stresses such as changes in osmolarity and metabolism, DNA damage, heat shock, ischaemia, or inflammatory cytokines3–6. Sek1 (JNKK/MKK4) is a direct activator of SAPKs/JNKs in response to environmental stresses or mitogenic factors7–9. Here we investigate the role of Sek1 in development and apoptosis by deleting sek1 in embryonic stem (ES) cells by homologous recombination. We provide genetic evidence that different stresses utilize distinct signalling pathways for SAPK/ JNK activation, sek1−/− /rag2−/− chimaeric mice have normal numbers of mature T cells but fewer immature CD4+CD8+ thymocytes. The sek1 mutation did not affect the induction of apoptosis in response to environmental stresses in ES and T cells: instead, sek1 protected thymocytes from CD95 (Fas)- and CD3-mediated apoptosis. These data indicate that SEK1 mediates survival signals in T-cell development.

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References

  1. Derijard, B. et al. Cell 76, 1025–1037 (1994).

    Article  CAS  Google Scholar 

  2. Kyriakis, J. M. et al. Nature 369, 156–160 (1994).

    Article  ADS  CAS  Google Scholar 

  3. Minden, A. et al. Science 266, 1719–1723 (1994).

    Article  ADS  CAS  Google Scholar 

  4. Pombo, C. M. et al. J. Biol. Chem. 269, 26546–26551 (1994).

    CAS  PubMed  Google Scholar 

  5. Westwick, J. K., Bielawska, A. E., Dbaido, G., Hannun, Y. A. & Brenner, D. A. J. Biol. Chem. 270, 22689–22692 (1995).

    Article  CAS  Google Scholar 

  6. Verheij, M. et al. Nature 380, 75–79 (1996).

    Article  ADS  CAS  Google Scholar 

  7. Sanchez, I. et al. Nature 372, 794–798 (1994).

    Article  ADS  CAS  Google Scholar 

  8. Derijard, B. et al. Science 267, 682–685 (1995).

    Article  ADS  CAS  Google Scholar 

  9. Lin, A. et al. Science 268, 286–290 (1995).

    Article  ADS  CAS  Google Scholar 

  10. Han, J., Lee, J. D., Bibbs, L. & Ulevitch, R. J. Science 265, 808–811 (1994).

    Article  ADS  CAS  Google Scholar 

  11. Moriguchi, T., Kawasaki, H., Matsuda, S., Gotoh, Y. & Nishida, E. J. Biol. Chem. 270, 12969–12972 (1995).

    Article  CAS  Google Scholar 

  12. Meier, R., Rouse, J., Cuenda, A., Nebreda, A. R. & Cohen, P. Eur. J. Biochem. 236, 796–805 (1996).

    Article  CAS  Google Scholar 

  13. Fischer, K. D. et al. Nature 374, 474–477 (1995).

    Article  ADS  CAS  Google Scholar 

  14. Zanke, B. W. et al. Curr. Biol. 6, 606–613 (1996).

    Article  CAS  Google Scholar 

  15. Xia, Z., Dickens, M., Raingeaud, J., Davis, R. J. & Greenberg, M. E. Science 270, 1326–1331 (1995).

    Article  ADS  CAS  Google Scholar 

  16. Kuida, K. et al. Science 267, 2000–2003 (1995).

    Article  ADS  CAS  Google Scholar 

  17. Smith, C. A., Williams, M., Kingston, R., Jenkinson, E. H. & Owen, J. J. T. Nature 337, 181–184 (1989).

    Article  ADS  CAS  Google Scholar 

  18. Kruisbeek, A. M. & Amsen, D. Curr. Opin. Immunol. 8, 233–244 (1996).

    Article  CAS  Google Scholar 

  19. Su, B. et al. Cell 77, 727–736 (1994).

    Article  Google Scholar 

  20. Schmid, I., Uittenbogaart, C. H. & Giorgi, J. V. Cytometry 15, 12–20 (1994).

    Article  CAS  Google Scholar 

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Nishina, H., Fischer, K., Radvanyi, L. et al. Stress-signalling kinase Sek1 protects thymocytes from apoptosis mediated by CD95 and CD3. Nature 385, 350–353 (1997). https://doi.org/10.1038/385350a0

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