Abstract
THE import of proteins from the cytosol into the mitochondrial matrix involves the concerted action of two separate import systems: the TOM system in the outer membrane, and the TIM system in the inner membrane1,2. Here we report that the inner-membrane system also sorts proteins to the intermembrane space. Some intermembrane-space proteins, such as cytochromes b2 and c1, are synthesized with a complex pre-sequence consisting of a positively charged matrix targeting signal followed by an uncharged sequence that acts as sorting signal for the intermem-brane space3. We show that this sorting signal can be efficiently crosslinked to an inner-membrane protein of relative molecular mass 11K after the mature part of the precursor has been sorted to the intermembrane space. The UK protein, which we term Tim11, is a component of the protein import system in the inner membrane.
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References
Pfanner, N. & Meijer, M. Curr. Biol. 5, 132–135 (1995).
Pfanner, N. et al. Trends Biochem. Sci. 21, 51–52 (1996).
Glick, B. S., Beasley, E. M. & Schatz, G. Trends Biochem. Sci. 17, 453–459 (1992).
van Loon, A. P. G. M., Brändli, A. W. & Schatz, G. Cell 44, 801–812 (1986).
Nunnari, J., Fox, T. & Walter, P. Science 262, 1997–2004 (1995).
Glick, B. S., Wachter, C., Reid, G. A. & Schatz, G. Protein Sci. 2, 1901–1917 (1993).
Glick, B. S. et al. Cell 69, 809–822 (1992).
Hartl, F.-U., Ostermann, J., Guiiard, B. & Neupert, W. Cell 51, 1027–1037 (1987).
Habeeb, A. F. S. A. Methods Enzymol. 25, 457–464 (1972).
Phil, A. & Lange, R. J. Biol. Chem. 237, 1356–1362 (1962).
Eilers, M. & Schatz, G. Nature 322, 228–232 (1986).
Wachter, C., Schatz, G. & Glick, B. S. Mol. Biol. Cell 5, 465–474 (1994).
Gärtner, F., Bömer, U., Guiard, B. & Pfanner, N. EMBO J. 14, 1349–1359 (1995).
Hwang, S. T., Wachter, C. & Schatz, G. J. Biol. Chem. 266, 21083–21089 (1991).
Wachter, C., Schatz, G. & Glick, B. S. EMBO J. 11, 4787–4794 (1992).
Jascur, T., Goldenberg, D. P., Vestweber, D. & Schatz, G. J. Biol. Chem. 267, 13636–13641 (1992).
Scherer, P. E., Manning-Krieg, U. C., Jenö, P., Schatz, G. & Horst, M. Proc. Natl Acad. Sci. USA 89, 11930–11934 (1992).
Ryan, K. R. & Jensen, R. E. J. Biol. Chem. 268, 23743–23746 (1993).
Kübrich, M. et al. FEBS Lett. 349, 222–228 (1994).
Beasley, E. M., Müller, S. & Schatz, G. EMBO J. 12, 2303–2311 (1993).
Gruhler, A., Ono, H., Guiard, B., Neupert, W. & Stuart, R. A. EMBO J. 14, 1349–1359 (1995).
Kaufmann, S. H. & Shaper, J. H. Exp. Cell Res. 155, 477–495 (1984).
Tokatlidis, K. et al. FEBS Lett. 282, 205–208 (1991).
Glick, B. S. & Pon, L. Methods Enzymol. 260, 213–223 (1995).
Wessel, D. & Glügge, U. I. Anal. Biochem. 138, 141–143 (1984).
Schägger, H. & von Jagow, G. Anal. Biochem. 166, 368–379 (1987).
Wach, A., Brachat, A., Pöhlmann, R. & Philippsen, P. Yeast 10, 1791–1808 (1994).
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Tokatlidis, K., Junne, T., Moes, S. et al. Translocation arrest of an intramitochondrial sorting signal next to Tim11 at the inner-membrane import site. Nature 384, 585–588 (1996). https://doi.org/10.1038/384585a0
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DOI: https://doi.org/10.1038/384585a0
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