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Crystal structure of a DExx box DNA helicase

Nature volume 384, pages 379–383 (1996)Cite this article

Abstract

THERE are a wide variety of helicases that unwind helical DNA1 and RNA substrates2. The twelve helicases that have been identified in Escherichia coli1 play a role in almost all cellular processes involving nucleic acids. We have solved the crystal structure of a monomeric form of a DNA helicase from Bacillus stearothermo-philus, alone and in a complex with ADP, at 2.5 and 2.9 Å resolution, respectively. The enzyme comprises two domains with a deep cleft running between them. The ATP-binding site, which is situated at the bottom of this cleft, is formed by motifs that are conserved across the superfamily of related helicases. Unexpected structural homo logy with the DNA recombination protein, RecA, suggests how ATP binding and hydrolysis may drive conformational changes of the enzyme during catalysis, and implies that there is a common mechanism for all helicases.

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References

  1. Lohman, T. M. & Bjornson, K. P. Annu. Rev. Biochem. 65, 169–214 (1996).

    CAS  Article  Google Scholar 

  2. Schmid, S. R. & Linder, P. Mol. Microbiol. 6, 283–292 (1992).

    CAS  Article  Google Scholar 

  3. Egelman, E. H. Structure 4, 759–762 (1996).

    CAS  Article  Google Scholar 

  4. Chao, K. & Lohman, T. M. J. Mol. Biol. 221, 1165–1181 (1991).

    CAS  Article  Google Scholar 

  5. Runyon, G. T., Wong, I. & Lohman, T. M. Biochemistry 32, 602–612 (1993).

    CAS  Article  Google Scholar 

  6. Schaeffer, L. et al. Science 260, 58–63 (1993).

    ADS  CAS  Article  Google Scholar 

  7. Iordanescu, S. Mol. Gen. Genet. 241, 185–192 (1993).

    CAS  Article  Google Scholar 

  8. Gorbalenya, A. E. & Koonin, E. V. Curr. Opin. Struct. Biol. 3, 419–429 (1993).

    CAS  Article  Google Scholar 

  9. Story, R. M., Weber, I. T. & Steitz, T. A. Nature 355, 318–325 (1992).

    ADS  CAS  Article  Google Scholar 

  10. Walker, J. E., Saraste, M., Runswick, M. J. & Gay, N. J. EMBO J. 1, 945–951 (1982).

    CAS  Article  Google Scholar 

  11. Pause, A. & Sonenburg, N. EMBO J. 7, 2643–2654 (1992).

    Article  Google Scholar 

  12. Gross, C. H. & Shuman, S. J. Virol. 69, 4727–4736 (1995).

    CAS  Google Scholar 

  13. Brosh, R. M. & Matson, S. W. J. Bacteriol. 177, 5612–5621 (1995).

    CAS  Article  Google Scholar 

  14. Rozen, F. et al. Mol. Cell. Biol. 10, 1134–1144 (1990).

    CAS  Article  Google Scholar 

  15. Washburn, J. D. & Kushner, S. R. J. Bacteriol. 175, 341–350 (1993).

    CAS  Article  Google Scholar 

  16. Story, R. M. & Steitz, T. A. Nature 355, 374–376 (1992).

    ADS  CAS  Article  Google Scholar 

  17. Wong, I. & Lohman, T. M. Science 256, 350–355 (1992).

    ADS  CAS  Article  Google Scholar 

  18. Stasiak, A., Egelman, E. H. & Howard-Flanders, P. J. Mol. Biol. 202, 659–662 (1988).

    CAS  Article  Google Scholar 

  19. Zhu, L. & Weller, S. K. Virology 166, 366–378 (1988).

    CAS  Article  Google Scholar 

  20. Crute, J. J., Mocarski, E. S. & Lehman, I. R. Nucleic Acids Res. 16, 6585–6596 (1988).

    CAS  Article  Google Scholar 

  21. Opperman, T. & Richardson, J. P. J. Bacteriol. 176, 6033–5043 (1994).

    Google Scholar 

  22. Abrahams, J.-P., Leslie, A. G. W., Lutter, R. & Walker, J. E. Nature 370, 621–628 (1994).

    ADS  CAS  Article  Google Scholar 

  23. Miwa, Y., Horiguchi, T. & Shigesada, K. J. Mol. Biol. 254, 815–837 (1995).

    CAS  Article  Google Scholar 

  24. Yu, X., Angov, E., Camerini-Otero, R. D. & Egelman, E. H. Biophys. J. 69, 2728–2738 (1995).

    ADS  CAS  Article  Google Scholar 

  25. Hingorani, M. M. & Patel, S. S. Biochemistry 35, 2218–2228 (1996).

    CAS  Article  Google Scholar 

  26. Bujalowski, W. & Klonowska, M. M. Biochemistry 32, 5888–5900 (1993).

    CAS  Article  Google Scholar 

  27. Zavitz, K. H. & Marians, K. J. J. Biol. Chem. 268, 4337–4346 (1993).

    CAS  PubMed  Google Scholar 

  28. Lee, M. S. & Marians, K. J. Proc. Natl Acad. Sci. USA 84, 8345–8349 (1987).

    ADS  CAS  Article  Google Scholar 

  29. Zavitz, K. H. & Marians, K. J. J. Biol. Chem. 267, 6933–6940 (1992).

    CAS  PubMed  Google Scholar 

  30. Brunger, A. T. Nature 355, 472–474 (1992).

    ADS  CAS  Article  Google Scholar 

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Author notes

  1. James A. Brannigan: Department of Chemistry, University of York, Heslington, YorkYOl 5DD, UK

Affiliations

  1. Laboratory of Molecular Biophysics, Rex Richards Building, University of Oxford, South Parks Road, Oxford, 0X1 3QU, UK

    Hosahalli S. Subramanya, Louise E. Bird, James A. Brannigan & Dale B. Wigley

Authors
  1. Hosahalli S. Subramanya
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  2. Louise E. Bird
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  3. James A. Brannigan
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  4. Dale B. Wigley
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Subramanya, H., Bird, L., Brannigan, J. et al. Crystal structure of a DExx box DNA helicase. Nature 384, 379–383 (1996). https://doi.org/10.1038/384379a0

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