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A meiotic recombination checkpoint controlled by mitotic checkpoint genes

Nature volume 383, pages840–843(1996)Cite this article

Abstract

IN budding yeast, meiotic recombination occurs at about 200 sites per cell and involves DNA double-strand break (DSB) intermediates1–3. Here we provide evidence that a checkpoint control requiring the mitotic DNA-damage checkpoint genes RAD17, RAD24 and MEC1 ensures that meiotic recombination is complete before the first meiotic division (MI). First, RAD17, RAD24 and MEC1 are required for the meiotic arrest caused by blocking the repair of DSBs with a mutation in the recA homologue DMC1. Second, mec1 and rad24 single mutants (DMC1+) appear to undergo MI before all recombination events are complete. Curiously, the mitosis-specific checkpoint gene RAD9 is not required for meiotic arrest of dmc1 mutants4. This shows that although mitotic and meiotic control mechanisms are related, they differ significantly. Rad17 and Rad24 proteins may contribute directly to formation of an arrest signal by association with single-strand DNA in mitosis and meiosis.

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Author information

Affiliations

  1. Department of Molecular and Cellular Biology, University of Arizona, Tucson, Arizona, 85721, USA

    David Lydall & Ted Weinert

  2. Department of Radiation and Cellular Oncology/Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, Illinois, 60638, USA

    Yuri Nikolsky & Douglas K. Bishop

Authors
  1. David Lydall
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  2. Yuri Nikolsky
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  3. Douglas K. Bishop
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  4. Ted Weinert
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Lydall, D., Nikolsky, Y., Bishop, D. et al. A meiotic recombination checkpoint controlled by mitotic checkpoint genes. Nature 383, 840–843 (1996). https://doi.org/10.1038/383840a0

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