Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Transcription-linked acetylation by Gcn5p of histones H3 and H4 at specific lysines

Abstract

THE yeast transcriptional adaptor1–3, Gcn5p, is a catalytic subunit of a nuclear (type A) histone acetyltransferase linking histone acetylation to gene activation4–6. Here we report that Gcn5p acetylates histones H3 and H4 non-randomly at specific lysines in the amino-terminal domains. Lysine 14 of H3 and lysines 8 and 16 of H4 are highly preferred acetylation sites for Gcn5p. We also demonstrate that lysine 9 is the preferred position of acetylation in newly synthesized yeast H3 in vivo. This finding, along with the fact that lysines 5 and 12 in H4 are predominant acetylation sites during chromatin assembly of many organisms7–11, indicates that Gcn5p acetylates a distinct set of lysines that do not overlap with those sites characteristically used by type B histone acetyltransferase s for histone deposition and chromatin assembly.

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Georgakopoulos, T. & Thireos, G. EMBO J. 11, 4145–4152 (1992).

    CAS  Article  Google Scholar 

  2. 2

    Berger, S. L., Cress, W. D., Cress, A., Triezenberg, S. J. & Guarente, L. Cell 61, 1199–1208 (1990).

    CAS  Article  Google Scholar 

  3. 3

    Berger, S. L. et al. Cell 70, 251–265 (1992).

    CAS  Article  Google Scholar 

  4. 4

    Brownell, J. E. et al. Cell 84, 843–851 (1996).

    CAS  Article  Google Scholar 

  5. 5

    Wolffe, A. P. & Pruss, D. Cell 84, 817–819 (1996).

    CAS  Article  Google Scholar 

  6. 6

    Brownell, J. E. & Allis, C. D. Curr. Opin. Genet. Dev. 6, 176–184 (1996).

    CAS  Article  Google Scholar 

  7. 7

    Chicoine, L. G., Schulman, I. G., Richman, R., Cook, R. G. & Allis, C. D. J. Biol. Chem. 261, 1071–1076 (1986).

    CAS  PubMed  Google Scholar 

  8. 8

    Sobel, R. E., Cook, R. G. & Allis, C. D. J. Biol. Chem. 269, 18576–18582 (1994).

    CAS  PubMed  Google Scholar 

  9. 9

    Munks, R. J., Moore, J., O'Neill, L. P. & Turner, B. M. FEBS Lett. 284, 245–248 (1991).

    CAS  Article  Google Scholar 

  10. 10

    Turner, B. M., O'Neill, L. P. & Allan, I. M. FEBS Lett. 253, 141–145 (1989).

    CAS  Article  Google Scholar 

  11. 11

    Thorne, A. W., Kmiciek, D., Mitchelson, K., Sautiere, P. & Crane-Robinson, C. Eur. J. Biochem. 193, 701–713 (1990).

    CAS  Article  Google Scholar 

  12. 12

    Clarke, D. J., O'Neill, L. P. & Turner, B. M. Biochem. J. 294, 557–561 (1993).

    CAS  Article  Google Scholar 

  13. 13

    Sobel, R. E., Cook, R. G., Perry, C. A., Annunziato, A. T. & Allis, C. D. Proc. Natl Acad. Sci. USA 92, 1237–1241 (1995).

    ADS  CAS  Article  Google Scholar 

  14. 14

    Candau, R. & Berger, S. L. J. Biol. Chem. 271, 5237–5245 (1996).

    CAS  Article  Google Scholar 

  15. 15

    Georgakopoulos, T., Gounalaki, N. & Thiros, G. Mol. Gen. Genet. 246, 723–728 (1995).

    CAS  Article  Google Scholar 

  16. 16

    Horiuchi, J., Silverman, N., Marcus, G. A. & Guarente, L. Mol. Cell. Biol. 15, 1203–1209 (1995).

    CAS  Article  Google Scholar 

  17. 17

    Yang, X.-J., Ogryzko, V. V., Nishikawa, J.-I., Howard, B. H. & Nakatani, Y. Nature 382, 319–324 (1996).

    ADS  CAS  Article  Google Scholar 

  18. 18

    Edmondson, D. G., Smith, M. M. & Roth, S. Y. Genes Dev. 10, 1247–1259 (1996).

    CAS  Article  Google Scholar 

  19. 19

    Gorovsky, M. A., Yao, M.-C., Keevert, J. B. & Pleger, G. L. Methods Cell Biol. 9, 311–327 (1975).

    CAS  Article  Google Scholar 

  20. 20

    Vavra, K. J., Allis, C. D. & Gorovsky, M. A. J. Biol. Chem. 257, 2591–2598 (1982).

    CAS  PubMed  Google Scholar 

  21. 21

    Braunstein, M., Sobel, R. E., Allis, C. D., Turner, B. M. & Broach, J. R. Mol. Cell. Biol. 16, 4349–4356 (1996).

    CAS  Article  Google Scholar 

  22. 22

    Horiuchi, K. & Fujimoto, D. Anal. Biochem. 69, 491–496 (1975).

    CAS  Article  Google Scholar 

  23. 23

    Brownell, J. E. & Allis, C. D. Proc. Natl Acad. Sci. USA 92, 6364–6368 (1995).

    ADS  CAS  Article  Google Scholar 

Download references

Author information

Affiliations

Authors

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Kuo, MH., Brownell, J., Sobel, R. et al. Transcription-linked acetylation by Gcn5p of histones H3 and H4 at specific lysines. Nature 383, 269–272 (1996). https://doi.org/10.1038/383269a0

Download citation

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing