Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

A new member of the frizzled family from Drosophila functions as a Wingless receptor

Nature volume 382, pages 225–230 (1996)Cite this article

Abstract

Receptors for Wingless and other signalling molecules of the Wnt gene family have yet to be identified. We show here that cultured Drosophila cells transfected with a novel member of the frizzled gene family in Drosophila, Dfz2, respond to added Wingless protein by elevating the level of the Armadillo protein. Moreover, Wingless binds to Drosophila or human cells expressing Dfz2. These data demonstrate that Dfz2 functions as a Wingless receptor, and they imply, in general, that Frizzled proteins are receptors for the Wnt signalling molecules.

This is a preview of subscription content, access via your institution

Relevant articles

Open Access articles citing this article.

Access options

Rent or buy this article

Prices vary by article type

from$1.95

to$39.95

Learn more

Prices may be subject to local taxes which are calculated during checkout

References

  1. Nusse, R. & Varmus, H. E. Cell 69, 1073–1087 (1992).

    Article  CAS  Google Scholar 

  2. Klingensmith, J. & Nusse, R. Devl Biol. 166, 396–414 (1994).

    Article  CAS  Google Scholar 

  3. Herman, M. A. & Horvitz, H. R. Development 120, 1035–1047 (1994).

    CAS  PubMed  Google Scholar 

  4. Rijsewijk, F. et al. Cell 50, 649–657 (1987).

    Article  CAS  Google Scholar 

  5. Baker, N. E. EMBO J. 6, 1765–1773 (1987).

    Article  CAS  Google Scholar 

  6. Perrimon, N. Cell 76, 781–784 (1994).

    Article  CAS  Google Scholar 

  7. Peifer, M. J. Cell Sci. 105, 993–1000 (1993).

    CAS  PubMed  Google Scholar 

  8. Bejsovec, A. & Wieschaus, E. Development 119, 501–517 (1993).

    CAS  PubMed  Google Scholar 

  9. Klingensmith, J., Nusse, R. & Perrimon, N. Genes Dev. 8, 118–130 (1994).

    Article  CAS  Google Scholar 

  10. Theisen, H. et al. Development 120, 347–360 (1994).

    CAS  PubMed  Google Scholar 

  11. McCrea, P. D., Turck, C. W. & Gumbiner, B. Science 254, 1359–1361 (1991).

    Article  ADS  CAS  Google Scholar 

  12. Siegfried, E., Wilder, E. L. & Perrimon, N. Nature 367, 76–80 (1994).

    Article  ADS  CAS  Google Scholar 

  13. Peifer, M., Sweeton, D., Casey, M. & Wieschaus, E. Development 120, 369–380 (1994).

    CAS  PubMed  Google Scholar 

  14. Noordermeer, J., Klingensmith, J., Perrimon, N. & Nusse, R. Nature 367, 80–83 (1994).

    Article  ADS  CAS  Google Scholar 

  15. Riggleman, B., Schedl, P. & Wieschaus, E. Cell 63, 549–560 (1990).

    Article  CAS  Google Scholar 

  16. Van Leeuwen, F., Harryman Samos, C. & Nusse, R. Nature 368, 342–344 (1994).

    Article  ADS  CAS  Google Scholar 

  17. Peel, D. J. & Milner, M. J. Wilhelm Roux's Arch. dev. Biol. 201, 120–123 (1992).

    Article  Google Scholar 

  18. Wang, Y. et al. J. biol. Chem. 271, 4468–4476 (1996).

    Article  CAS  Google Scholar 

  19. Adler, P. N., Charlton, J. & Vinson, C. Devl Genet. 8, 99–119 (1987).

    Article  Google Scholar 

  20. Gubb, D. & Garcia, B. A. J. Embryol. exp. Morphol. 68, 37–57 (1982).

    CAS  PubMed  Google Scholar 

  21. Zheng, L., Zhang, J. J. & Carthew, R. W. Development 121, 3045–3055 (1995).

    CAS  PubMed  Google Scholar 

  22. Vinson, C. R., Conover, S. & Adler, P. N. Nature 338, 263–264 (1989).

    Article  ADS  CAS  Google Scholar 

  23. Adler, P. N., Vinson, C., Park, W. J., Conover, S. & Klein, L. Genetics 126, 401–416 (1990).

    CAS  PubMed  PubMed Central  Google Scholar 

  24. Vinson, C. R. & Adler, P. N. Nature 329, 549–551 (1987).

    Article  ADS  CAS  Google Scholar 

  25. Krasnow, R. E., Wong, L. L. & Adler, P. N. Development 121, 4095–4102 (1995).

    CAS  PubMed  Google Scholar 

  26. Yanagawa, S., van Leeuwen, F., Wodarz, A., Klingensmith, J. & Nusse, R. Genes Dev. 9, 1087–1097 (1995).

    Article  CAS  Google Scholar 

  27. Olwin, B. B. & Rapraeger, A. J. Cell Biol. 118, 631–639 (1992).

    Article  CAS  Google Scholar 

  28. Johnson, D. E. & Williams, L. T. Adv. Cancer Res. 60, 1–41 (1993).

    CAS  PubMed  Google Scholar 

  29. Seifert, R. A. et al. J. biol. Chem. 264, 8771–8778 (1989).

    CAS  PubMed  Google Scholar 

  30. Ten Dijke, P., Miyazono, K. & Heldin, C.-H. Curr. Opin. Cell Biol. 8, 139–145 (1996).

    Article  CAS  Google Scholar 

  31. Couso, J. P. & Martinez Arias, A. Cell 79, 259–272 (1994).

    Article  CAS  Google Scholar 

  32. Russell, J., Gennissen, A. & Nusse, R. Development 115, 475–485 (1992).

    CAS  PubMed  Google Scholar 

  33. Graba, Y. et al. Development 121, 209–218 (1995).

    CAS  PubMed  Google Scholar 

  34. Eisenberg, L. M., Ingham, P. W. & Brown, A. M. C. Devl Biol. 154, 73–83 (1992).

    Article  CAS  Google Scholar 

  35. Gomperts, S. N. Cell 84, 659–662 (1996).

    Article  CAS  Google Scholar 

  36. Khorana, H. J. biol. Chem. 267, 1–4 (1992).

    CAS  PubMed  Google Scholar 

  37. Kyte, J. & Doolittle, R. J. molec. Biol. 157, 105–132 (1982).

    Article  CAS  Google Scholar 

  38. Sambrook, J., Fritsch, E. F. & Maniatis, T. Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Laboratory Press, NY, 1989).

    Google Scholar 

  39. Auffray, C. & Rougeon, F. Eur. J. Biochem. 107, 303–314 (1980).

    Article  CAS  Google Scholar 

  40. Campos-Ortega, J. A. & Hartenstein, V. The Embryonic Development of Drosophila melanogaster (Springer, Berlin, 1985).

    Book  Google Scholar 

  41. Mathies, L. D., Kerridge, S. & Scott, M. P. Development 120, 2799–2809 (1994).

    CAS  PubMed  Google Scholar 

  42. Bienz, M. Trends Genet. 10, 22–26 (1994).

    Article  CAS  Google Scholar 

  43. Tautz, D. & Pfeiffle, C. Chromosoma 98, 81–85 (1989).

    Article  CAS  Google Scholar 

  44. Reuter, R., Panganiban, G. E. F., Hoffmann, F. M. & Scott, M. P. Development 110, 1031–1040 (1990).

    CAS  PubMed  Google Scholar 

  45. Koelle, M. R. et al. Cell 67, 59–77 (1991).

    Article  CAS  Google Scholar 

  46. Oda, H. et al. J. Cell Biol. 121, 1133–1140 (1993).

    Article  CAS  Google Scholar 

  47. Gorman, C., Gies, D. & McCray, G. DNA Prot. Engng Technol. 2, 3–9 (1990).

    Google Scholar 

  48. Fehon, R. G. et al. Cell 61, 523–534 (1990).

    Article  CAS  Google Scholar 

  49. Evan, G. I., Lewis, G. K., Ramsay, G. & Bishop, J. M. Molec. cell. Biol. 5, 3610–3616 (1985).

    Article  CAS  Google Scholar 

  50. Caras, I. W. & Weddell, G. N. Science 243, 1196–1198 (1989).

    Article  ADS  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, 725 North Wolfe Street, Room 805 PCTB, Baltimore, Maryland, 21205, USA

    Purnima Bhanot, Jen-Chih Hsieh, Yanshu Wang, Jennifer P. Macke & Jeremy Nathans

  2. Department of Cell Biology and Anatomy, Johns Hopkins University School of Medicine, 725 North Wolfe Street, Room 805 PCTB, Baltimore, Maryland, 21205, USA

    Deborah Andrew

  3. Department of Neuroscience, Johns Hopkins University School of Medicine, 725 North Wolfe Street, Room 805 PCTB, Baltimore, Maryland, 21205, USA

    Jeremy Nathans

  4. Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, 725 North Wolfe Street, Room 805 PCTB, Baltimore, Maryland, 21205, USA

    Jen-Chih Hsieh, Yanshu Wang, Jennifer P. Macke & Jeremy Nathans

  5. Howard Hughes Medical Institute and Department of Developmental Biology, Stanford University School of Medicine, Stanford, California, 94305, USA

    Marcel Brink, Cindy Harryman Samos & Roel Nusse

Authors
  1. Purnima Bhanot
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Marcel Brink
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Cindy Harryman Samos
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jen-Chih Hsieh
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yanshu Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Jennifer P. Macke
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Deborah Andrew
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Jeremy Nathans
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Roel Nusse
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Jeremy Nathans or Roel Nusse.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Bhanot, P., Brink, M., Samos, C. et al. A new member of the frizzled family from Drosophila functions as a Wingless receptor. Nature 382, 225–230 (1996). https://doi.org/10.1038/382225a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/382225a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing