Letter | Published:

A histone octamer-like structure within TFIID

Abstract

THE general transcription factor TFIID nucleates initiation complex formation through direct core promoter binding1,2, commits promoters within chromatin to transcription3, and mediates the action of transcriptional activators, a phenomenon that may correlate with enhanced TFIID recruitment4–7 or conformational changes in TFIID-promoter complexes8,9. Molecular studies of the multiprotein TFIID complex have identified a primary TATA binding subunit (TBP)2, TBP-associated factors (TAFs) that interact with and mediate the function of activators2,7,10,11 and intersubunit interactions2 but have yielded relatively little insight into the structural organization of the complex or the actual mechanism of transcriptional activation. Here we present biochemical evidence for the structural relevance of histone homo-logies in the human TFIID subunits hTAFSO, hTAF31 and hTAF20/15. Together with analyses of native TFIID complexes and accompanying crystallographic studies12, the results suggest that there is a histone octamer-like TAF complex within TFIID.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Roeder, R. G. Trends biochem, Sci. 16, 402–408 (1991).

  2. 2

    Burley, S. K. & Roeder, R. G. A. Rev. Biochem. (in the press).

  3. 3

    Owen-Hughes, T. & Workman, J. L. Crit. Rev. euk. Gene Express. 4, 403–441 (1994).

  4. 4

    Abmayr, S. M. Workman, J. L. & Roeder, R. G. Genes Dev. 2, 542–553 (1998).

  5. 5

    Workman, J. L., Abmayr, S. M., Cromlish, W. A. & Roeder, R. G. Cell 55, 211–219 (1988).

  6. 6

    Lieberman, P. M. & Berk, A. J. Genes Dev. 8, 995–1006 (1994).

  7. 7

    Sauer, F., Hansen, S. K. & Tjian, R. Science 270, 1783–1788 (1995).

  8. 8

    Horikoshi, M., Hai, T., Lin, Y.-S., Green, M. R. & Roeder, R. G. Cell 54, 1033–1042 (1988).

  9. 9

    Horikoshi, M., Carey, M. F., Kakidani, H. & Roeder, R. G. Cell 54, 665–669 (1988).

  10. 10

    Chen, J.-L., Attardi, L. D., Verrijzer, C. P., Yokomori, K. & Tjian, R. Cell 79, 93–105 (1994).

  11. 11

    Jacq, X. et al. Cell 79, 107–117 (1994).

  12. 12

    Xie, X. et al. Nature 380, 316–322 (1996).

  13. 13

    Mengus, G. et al. EMBO J. 14, 1520–1531 (1995).

  14. 14

    Yokomori, K., Chen, J.-L., Admon, A., Zhou, S. & Tjian, R. Genes Dev. 7, 2587–2597 (1993).

  15. 15

    Kokubo, T. et al. Nature 367, 484–487 (1994).

  16. 16

    Hisatake, K. et al. Proc. natn. Acad. Sci. U.S.A. 92, 8195–8199 (1995).

  17. 17

    Baxevanis, A. D., Arents, G., Moudrianakis, E. N. & Landsman, D. Nucleic Acids Res. 23, 2685–2691 (1995).

  18. 18

    Arents, G., Burlingame, R. W., Wang, B.-C., Love, W. E. & Moudrianakis, E. N. Proc. natn. Acad. Sci. U.S.A. 88, 10148–10152 (1991).

  19. 19

    Pruss, D., Hayes, J. J. & Wolffe, A. P. BioEssays 17, 1–10 (1995).

  20. 20

    Chiang, C.-M., Ge, H., Wang, Z., Hoffmann, A. & Roeder, R. G. EMBO J. 12, 2749–2762 (1993).

  21. 21

    Thut, C. J., Chen, J.-L., Klemm, R. & Tjian, R. Science 267, 100–104 (1995).

  22. 22

    Chiang, C.-M. & Roeder, R. G. Science 267, 531–536 (1995).

  23. 23

    Arents, G. & Moudrianakis, E. N. Proc. natn. Acad. Sci. U.S.A. 90, 10489–10493 (1993).

  24. 24

    Sawadogo, M. & Roeder, R. G. Cell 43, 165–175 (1985).

  25. 25

    Nakajima, N., Horikoshi, M. & Roeder, R. G. Molec. cell. Biol. 8, 4028–4040 (1988).

  26. 26

    Gilmour, D. S., Dietz, T. J. & Elgin, S. C. R. Molec. cell. Biol. 10, 4233–4238 (1990).

  27. 27

    Nakatani, Y. et al. Nature 348, 86–88 (1990).

  28. 28

    Martinez, E. et al. Proc. natn. Acad. Sci. U.S.A. 92, 11864–11868 (1995).

  29. 29

    Poon, D. et al. Proc. natn. Acad. Sci. U.S.A. 92, 8224–8228 (1995).

  30. 30

    Zhong, R., Roeder, R. G. & Heintz, N. Nucleic Acids Res. 11, 7409–7425 (1983).

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.