Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Sheep cloned by nuclear transfer from a cultured cell line

Nature volume 380, pages 64–66 (1996)Cite this article

Abstract

NUCLEAR transfer has been used in mammals as both a valuable tool in embryological studies1 and as a method for the multiplication of 'elite' embryos2–4. Offspring have only been reported when early embryos, or embryo-derived cells during primary culture, were used as nuclear donors5,6. Here we provide the first report, to our knowledge, of live mammalian offspring following nuclear transfer from an established cell line. Lambs were born after cells derived from sheep embryos, which had been cultured for 6 to 13 passages, were induced to quiesce by serum starvation before transfer of their nuclei into enucleated oocytes. Induction of quiescence in the donor cells may modify the donor chromatin structure to help nuclear reprogramming and allow development. This approach will provide the same powerful opportunities for analysis and modification of gene function in livestock species that are available in the mouse through the use of embryonic stem cells7.

This is a preview of subscription content, access via your institution

Relevant articles

Open Access articles citing this article.

Access options

Buy article

Get time limited or full article access on ReadCube.

$32.00

Buy

All prices are NET prices.

References

  1. McGrath, J. & Solter, D. Science 220, 1300–1302 (1983).

    Article  ADS  CAS  Google Scholar 

  2. Bondioli, K. R., Westhusin, M. E. & Looney, C. R. Therio 33, 165–174 (1990).

    Article  Google Scholar 

  3. Prather, R. S. & First, N. L. Int. Rev. Cytol. 120, 169–190 (1990).

    Article  CAS  Google Scholar 

  4. Chesne, P., Heyman, Y., Peynot, N. & Renard, J.-P. C.r. Acad. Sci. Paris Life Sci. 316, 487–491 (1993).

    CAS  Google Scholar 

  5. Sims, M. & First, N. L. Proc. natn. Acad. Sci. U.S.A. 91, 6243–6147 (1994).

    Article  Google Scholar 

  6. Collas, P. & Barnes, F. L. Molec. Repr. Dev. 38, 264–267 (1994).

    Article  CAS  Google Scholar 

  7. Hooper, M. L. Embryonal Stem Cells: Introducing Planned Changes into the Germline (ed. Evans, H. J.) (Harwood Academic, Switzerland, 1992).

    Google Scholar 

  8. Galli, C., Lazzari, G., Flechon, J. & Moor, R. M. Zygote 2, 385–389 (1994).

    Article  CAS  Google Scholar 

  9. Campbell, K. H. S., Ritchie, W. A. & Wilmut, I. Biol. Reprod. 49, 933–942 (1993).

    Article  CAS  Google Scholar 

  10. Campbell, K. H. S., Loi, P., Capai, P. & Wilmut, I. Biol. Reprod. 50, 1385–1393 (1994).

    Article  CAS  Google Scholar 

  11. Szollosi, D., Czolowska, R., Szollosi, M. S. & Tarkowski, A. K. J. Cell Sci. 91, 603–613 (1988).

    PubMed  Google Scholar 

  12. Whitten, W. K. & Biggers, J. D. J. Reprod. Fertil. 17, 399–401 (1968).

    Article  CAS  Google Scholar 

  13. Breslaw, N. E. & Clayton, D. G. J. Am. Stat. Assoc. 88, 9–25 (1993).

    Google Scholar 

  14. Whitfield, J. F., Boynton, A. L., Rixon, R. H. & Youdale, T. Control of Animal Cell Proliferation Vol. 1 (eds Boynton, A. L. & Leffert, H. L.) 331–365 (Academic, London 1985).

    Book  Google Scholar 

  15. Piedrahita, J. A., Anderson, G. B. & Bon Durrant, R. H. Therio 34, 879–901 (1990).

    Article  CAS  Google Scholar 

  16. Gerfen, R. W. & Wheeler, M. B. Anim. Biotechnol. 6, 1–14 (1995).

    Article  Google Scholar 

  17. Willadsen, S. M. Nature 320, 63–65 (1986).

    Article  ADS  CAS  Google Scholar 

  18. Buchanan, F. C., Galloway, S. M. & Crawford, A. M. Anim. Genet. 25, 60 (1994).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Roslin Institute (Edinburgh), Roslin, Midlothian, EH25 9PS, UK

    K. H. S. Campbell, J. McWhir, W. A. Ritchie & I. Wilmut

Authors
  1. K. H. S. Campbell
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. McWhir
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. W. A. Ritchie
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. I. Wilmut
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Campbell, K., McWhir, J., Ritchie, W. et al. Sheep cloned by nuclear transfer from a cultured cell line. Nature 380, 64–66 (1996). https://doi.org/10.1038/380064a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/380064a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing