Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Role for a Xenopus Orc2-related protein in controlling DNA replication

Nature volume 379, pages 357–360 (1996)Cite this article

Abstract

THE six-subunit origin recognition complex (ORC) is essential for the initiation of DNA replication at specific origins in the budding yeast Saccharomyces cerevisiae1–9. An important issue is whether DNA replication in higher eukaryotes, in which the characteristics of replication origins are poorly defined10, occurs by an ORC-dependent mechanism. We have identified a Xenopus laevis Orel-related protein (XORC2) by its ability to rescue a mitotic-catastrophe mutant of the fission yeast Schizosaccharomyces pombe. We show that immunodepletion of XORC2 from Xenopus egg extracts11–13 abolishes the replication of chromosomal DNA but not elongation synthesis on a single-stranded DNA template. Indirect immunofluorescence indicates that XORC2 binds to chromatin well before the commencement of DNA synthesis, and even under conditions that prevent the association of replication licensing factor(s) with the DNA. These findings suggest that Orc2 plays an important role at an early step of chromosomal replication in animal cells.

This is a preview of subscription content, access via your institution

Access options

Rent or buy this article

Prices vary by article type

from$1.95

to$39.95

Learn more

Prices may be subject to local taxes which are calculated during checkout

References

  1. Bell, S. P. & Stillman, B. Nature 357, 128–134 (1992).

    Article  ADS  CAS  PubMed  Google Scholar 

  2. Diffley, J. F. X. & Cocker, J. H. Nature 357, 169–172 (1992).

    Article  ADS  CAS  PubMed  Google Scholar 

  3. Bell, S. P., Kobayashi, R. & Stillman, B. Science 262, 1844–1849 (1993).

    Article  ADS  CAS  PubMed  Google Scholar 

  4. Foss, M., McNally, F. J., Laurenson, P. & Rine, J. Science 262, 1838–1843 (1993).

    Article  ADS  CAS  PubMed  Google Scholar 

  5. Micklem, G., Rowley, A., Harwood, J., Nasmyth, K. & Diffley, J. F. X. Nature 366, 87–89 (1993).

    Article  ADS  CAS  PubMed  Google Scholar 

  6. Li, J. J. & Herskowitz, I. Science 262, 1870–1874 (1993).

    Article  ADS  CAS  PubMed  Google Scholar 

  7. Diffley, J. F. X., Cocker, J. H., Dowell, S. J. & Rowley, A. Cell 78, 303–316 (1994).

    Article  CAS  PubMed  Google Scholar 

  8. Fox, C. A., Loo, S., Dillin, A. & Rine, J. Genes Dev. 9, 911–924 (1995).

    Article  CAS  PubMed  Google Scholar 

  9. Liang, C., Weinreich, M. & Stillman, B. Cell 81, 667–676 (1995).

    Article  CAS  PubMed  Google Scholar 

  10. Coverley, D. & Laskey, R. A. A. Rev. Biochem. 63, 745–776 (1994).

    Article  CAS  Google Scholar 

  11. Blow, J. J. & Laskey, R. A. Cell 47, 577–587 (1986).

    Article  CAS  PubMed  Google Scholar 

  12. Newport, J. Cell 48, 205–217 (1987).

    Article  CAS  PubMed  Google Scholar 

  13. Adachi, Y. & Laemmli, U. K. EMBO J. 13, 4153–4164 (1994).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Lundgren, K. et al. Cell 64, 1111–1122 (1991).

    Article  CAS  PubMed  Google Scholar 

  15. Russell, P. & Nurse, P. Cell 49, 559–567 (1987).

    Article  CAS  PubMed  Google Scholar 

  16. Zuber, M., Yasui, W., Tan, E. M. & Ryoji, M. Expl Cell Res. 182, 384–393 (1989).

    Article  CAS  Google Scholar 

  17. Méchali, M. & Harland, R. M. Cell 30, 93–101 (1982).

    Article  PubMed  Google Scholar 

  18. Kornbluth, S., Smythe, C. & Newport, J. W. Molec. cell. Biol. 12, 3216–3223 (1992).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  19. Fang, F. & Newport, J. W. J. Cell Sci. 106, 983–994 (1993).

    CAS  PubMed  Google Scholar 

  20. Chong, J. P. J., Mahbubani, H. M., Khoo, C.-Y. & Blow, J. J. Nature 375, 418–421 (1995).

    Article  ADS  CAS  PubMed  Google Scholar 

  21. Kubota, Y., Mimura, S., Nishimoto, S. I., Takisawa, H. & Nojima, H. Cell 81, 601–609 (1995).

    Article  CAS  PubMed  Google Scholar 

  22. Madine, M. A., Khoo, C.-Y., Mills, A. D. & Laskey, R. A. Nature 375, 421–424 (1995).

    Article  ADS  CAS  PubMed  Google Scholar 

  23. Dasso, M. & Newport, J. W. Cell 61, 811–823 (1990).

    Article  CAS  PubMed  Google Scholar 

  24. Mueller, P. R., Coleman, T. R. & Dunphy, W. G. Molec. Biol. Cell 6, 119–134 (1995).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  25. Maundrell, K. J. biol. Chem. 265, 10857–10864 (1990).

    CAS  PubMed  Google Scholar 

  26. Moreno, S., Klar, A. & Nurse, P. Meth. Enzym. 194, 795 (1991).

    Article  CAS  PubMed  Google Scholar 

  27. Allshire, R. C. Proc natn. Acad. Sci. U.S.A. 87, 4043–4047 (1990).

    Article  ADS  CAS  Google Scholar 

  28. Kumagai, A. & Dunphy, W. G. Molec. Biol. Cell 6, 199–213 (1995).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  29. Yan, H. & Newport, J. J. Cell Biol. 129, 1–15 (1995).

    Article  CAS  PubMed  Google Scholar 

  30. Mills, A. D. et al. J. Cell Sci. 94, 471–477 (1989).

    PubMed  Google Scholar 

Download references

Author information

Author notes

  1. William G. Dunphy: To whom correspondence should be addressed.

Authors and Affiliations

  1. Division of Biology 216-76, Howard Hughes Medical Institute, California Institute of Technology, Pasadena, California, 91125, USA

    Phillip B. Carpenter, Paul R. Mueller & William G. Dunphy

Authors
  1. Phillip B. Carpenter
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Paul R. Mueller
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. William G. Dunphy
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Carpenter, P., Mueller, P. & Dunphy, W. Role for a Xenopus Orc2-related protein in controlling DNA replication. Nature 379, 357–360 (1996). https://doi.org/10.1038/379357a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/379357a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing