Ataxia and epileptic seizures in mice lacking type 1 inositol 1,4,5-trisphosphate receptor

Nature volume 379, pages168–171(1996)Cite this article

Abstract

THE inositol 1,4,5-trisphosphate (InsP3) receptor acts as an InsP3-gated Ca2+ release channel in a variety of cell types1,2. Type 1 InsP3 receptor (IP3R1) is the major neuronal member of the IP3R family in the central nervous system3,4, predominantly enriched in cerebellar Purkinje cells but also concentrated in neurons in the hippocampal CA1 region, caudate-putamen, and cerebral cortex5,6. Here we report that most IP3R1-deficient mice generated by gene targeting die in utero, and born animals have severe ataxia and tonic or tonic-clonic seizures and die by the weaning period. An electroencephalogram showed that they suffer from epilepsy, indicating that IP3R1 is essential for proper brain function. However, observation by light microscope of the haematoxylin–eosin staining of the brain and peripheral tissues of IP3R1-deficient mice showed no abnormality, and the unique electrophysiological properties of the cerebellar Purkinje cells of IP3R1-deficient mice were not severely impaired.

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Author notes

  1. S. Sakakibara & H. Okano

    Present address: Department of Molecular Neurobiology, Institute of Basic Medical Sciences, and Center of TARA, University of Tsukuba, Tsukuba, Ibaraki, 305, Japan

  2. S. K. Mikoshiba: To whom correspondence should be addressed.

Affiliations

  1. Department of Molecular Neurobiology, Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo, 108, Japan

    M. Matsumoto, T. Nakagawa, T. Inoue, S. Sakakibara, M. Yamada, H. Yoneshima, A. Miyawaki, Y. Furuichi, H. Okano & S. K. Mikoshiba

  2. Department of Cell Biology, Cancer Institute, Toshima-ku, Tokyo, 170, Japan

    M. Matsumoto, T. Nakagawa, H. Takano, J. Kuno & T. Noda

  3. Department of Neurology, School of Medicine, Keio University, Shinjuku-ku, Tokyo, 160, Japan

    E. Nagata & K. Tanaka

  4. Molecular Neurobiology Laboratory, Tsukuba Life Science Center, The Institute of Physical and Chemical Research (RIKEN), Koyadai, Tsukuba-shi, Ibaraki, 305, Japan

    S. K. Mikoshiba

  5. Calciosignal Net Project, Exploratory Research for Advanced Technology (ERATO), JRDC 2-9-3 Shimo-meguro, Meguro-ku, Tokyo, 153, Japan

    S. K. Mikoshiba

Authors
  1. M. Matsumoto
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  2. T. Nakagawa
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  3. T. Inoue
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  4. E. Nagata
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  5. K. Tanaka
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  6. H. Takano
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  7. J. Kuno
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  8. S. Sakakibara
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  9. M. Yamada
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  10. H. Yoneshima
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  11. A. Miyawaki
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  12. Y. Furuichi
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  13. H. Okano
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  14. S. K. Mikoshiba
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  15. T. Noda
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Matsumoto, M., Nakagawa, T., Inoue, T. et al. Ataxia and epileptic seizures in mice lacking type 1 inositol 1,4,5-trisphosphate receptor. Nature 379, 168–171 (1996). https://doi.org/10.1038/379168a0

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