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Activation of a cell-cycle-regulated histone gene by the oncogenic transcription factor IRF-2

Abstract

THE human histone H4 gene FO108 is regulated during the cell cycle with a peak in transcription during early S phase1,2. The cell-cycle element (CCE) required for H4 histone activation is a sequence of 11 base pairs that binds a protein factor in electrophor-etic mobility shift assays that has been designated histone nuclear factor M (HiNF-M)2,3. Here we report the purification of HiNF-M, and show it to be a protein of relative molecular mass (Mr) 48K that is identical to interferon (IFN) regulatory factor 2 (IRF-2), a negative transcriptional regulator of the IFN response4. Recombinant IRF-2 (as well as the related protein IRF-1 (ref. 5)) binds the CCE specifically and activates transcription of this H4 histone gene. IRF-2 has been shown to have oncogenic potential6, and our results demonstrate a link between IRF-2 and a gene that is functionally coupled to DNA replication and cell-cycle progression at the Gl/S phase transition.

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References

  1. Plumb, M. A., Stein, J. L. & Stein, G. S. Nucleic Acids Res. 11, 2391–2401 (1983).

    Article  CAS  Google Scholar 

  2. Ramsey-Ewing, A., van Wijnen, A. J., Stein, G. S. & Stein, J. L. Proc. natn. Acad. Sci. U.S.A. 91, 4475–4479 (1994).

    Article  ADS  CAS  Google Scholar 

  3. van Wijnen, A. J., van den Ent, F.M.I., Lian, J. B., Stein, J. L. & Stein, G. S. Molec cell. Biol. 12, 3273–3287 (1992).

    Article  CAS  Google Scholar 

  4. Harada, H. et al. Cell 58, 729–739 (1989).

    Article  CAS  Google Scholar 

  5. Miyamoto, M. et al. Cell 54, 903–913 (1988).

    Article  CAS  Google Scholar 

  6. Harada, H. et al. Science 259, 971–974 (1993).

    Article  ADS  CAS  Google Scholar 

  7. Pauli, U., Chrysogelos, S., Stein, G., Stein, J. & Nick, H. Science 236, 1308–1311 (1987).

    Article  ADS  CAS  Google Scholar 

  8. van Wijnen, A. J. et al. Proc. natn. Acad. Sci. U.S.A. 91, 12882–12886 (1994).

    Article  ADS  CAS  Google Scholar 

  9. Itoh, S., Harada, H., Fujita, T., Mimura, T. & Taniguchi, T. Nucleic Acids Res. 17, 8372 (1989).

    Article  CAS  Google Scholar 

  10. Tanaka, N., Kawakami, T. & Taniguchi, T. Molec. cell. Biol. 13, 4531–4538 (1993).

    Article  CAS  Google Scholar 

  11. Veals, S. A. et al. Molec. cell. Biol. 12, 3315–3324 (1992).

    Article  CAS  Google Scholar 

  12. Driggers, P. H. et al. Proc. natn. Acad. Sci. U.S.A. 87, 3743–3747 (1990).

    Article  ADS  CAS  Google Scholar 

  13. Darnell, J. E. Jr, Kerr, I. M. & Stark, G. R. Science 264, 1415–1421 (1994).

    Article  ADS  CAS  Google Scholar 

  14. Palombella, V. J. & Maniatis, T. Molec. cell. Biol. 12, 3325–3336 (1992).

    Article  CAS  Google Scholar 

  15. Yamamoto, H., Lamphier, M. S., Fujita, T., Taniguchi, T. & Harada, H. Oncogene 9, 1423–1428 (1994).

    CAS  PubMed  Google Scholar 

  16. Hahn, S. Curr. Biol. 2, 152–154 (1992).

    Article  CAS  Google Scholar 

  17. Sussel, L. & Shore, D. Proc. natn. Acad. Sci. U.S.A. 88, 7749–7753 (1991).

    Article  ADS  CAS  Google Scholar 

  18. Lehming, N. et al. Nature 371, 175–179 (1994).

    Article  ADS  CAS  Google Scholar 

  19. Shakoori, R. et al., J. cell. Biochem. (in the press).

  20. Abdollahi, A., Lord, K. A., Hoffman-Lieberman, B. & Lieberman, D. Cell Growth Differ. 2, 401–407 (1991).

    CAS  PubMed  Google Scholar 

  21. Fujita, T. et al. Proc. natn. Acad. Sci. U.S.A. 86, 9936–9940 (1989).

    Article  ADS  CAS  Google Scholar 

  22. Ausubel, F. M. et al. (eds) Current Protocols in Molecular Biology (Wiley, New York, 1994).

  23. Laemmli, U. K. Nature 227, 680–685 (1970).

    Article  ADS  CAS  Google Scholar 

  24. Frappier, L. & O'Donnell, M. J. biol. Chem. 266, 7819–7826 (1991).

    CAS  PubMed  Google Scholar 

  25. Pine, R., Decker, T., Kessler, D. S., Levy, D. E. & Darnell, J. E. Jr Molec. cell. Biol. 10, 2448–2457 (1990).

    Article  CAS  Google Scholar 

  26. Tanaka, N. et al. Cell 77, 829–839 (1994).

    Article  CAS  Google Scholar 

  27. Matsuyama, T. et al. Cell 75, 83–97 (1993).

    Article  CAS  Google Scholar 

  28. Pen̄a, A. et al. J. biol. Chem. 268, 27277–27285 (1993).

    PubMed  Google Scholar 

  29. Lopata, M. A., Cleveland, D. W. & Sollner-Webb, B. Nucleic Acids Res. 12, 5707–5717 (1984).

    Article  CAS  Google Scholar 

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Vaughan, P., Aziz, F., van Wijnen, A. et al. Activation of a cell-cycle-regulated histone gene by the oncogenic transcription factor IRF-2 . Nature 377, 362–365 (1995). https://doi.org/10.1038/377362a0

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