Angiogenesis mediated by soluble forms of E-selectin and vascular cell adhesion molecule-1

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Abstract

ENDOTHELIAL adhesion molecules facilitate the entry of leukocytes into inflamed tissues. This in turn promotes neovascularization, a process central to the progression of rheumatoid arthritis, tumour growth and wound repair1. Here we test the hypothesis that soluble endothelial adhesion molecules promote angiogenesis2á¤-4. Human recombinant soluble E-selectin and soluble vascular cell adhesion molecule-1 induced chemotaxis of human endothelial cells in vitro and were angiogenic in rat cornea. Soluble E-selectin acted on endothelial cells in part through a sialyl Lewis-X-dependent mechanism, while soluble vascular cell adhesion molecule-1 acted on endothelial cells in part through a very late antigen (VLA)-4 dependent mechanism. The chemotactic activity of rheumatoid synovial fluid for endothelial cells, and also its angiogenic activity, were blocked by antibodies to either soluble E-selectin or soluble vascular cell adhesion molecule-1. These results suggest a novel function for soluble endothelial adhesion molecules as mediators of angiogenesis.

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References

  1. 1

    Folkman, J. & Shing, Y. J. biol. Chem. 267, 10931–10934 (1992).

  2. 2

    Lobb, R. R. et al. J. Immun. 147, 124–129 (1991).

  3. 3

    Lo, S. K. et al. J. exp. Med. 173, 1493–1500 (1991).

  4. 4

    Pober, J. S. et al. J. Immun. 136, 1680–1687 (1986).

  5. 5

    Koch, A. E. et al. Science 258, 1798–1801 (1992).

  6. 6

    Munro, J. M. et al. Am. J. Path. 141, 1397–1408 (1992).

  7. 7

    Paavonen, T. & Renkonen, R. Am. J. Path. 141, 1259–1264 (1992).

  8. 8

    Gillard, B. K., Jones, M. A. & Marcus, D. M. Archs Biochem. Biophys. 256, 435–445 (1987).

  9. 9

    Massia, S. P. & Hubbell, J. A. J. biol. Chem. 267, 14109–14206 (1992).

  10. 10

    Johnson, B. A., Haines, G. K., Harlow, L. A. & Koch, A. E. Arthritis Rheum. 36, 137–146 (1993).

  11. 11

    Brooks, P. C., Clark, R. A. & Cheresh, D. A. Science 264, 569–571 (1994).

  12. 12

    Gamble, J. R. et al. J. Cell Biol. 121, 931–943 (1993).

  13. 13

    Nguyen, M., Strubel, N. A. & Bischoff, J. Nature 365, 267–269 (1993).

  14. 14

    Koch, A. E. et al. Lab. Invest. 64, 313–320 (1991).

  15. 15

    Koch, A. E., Turkiewicz, W., Harlow, L. A. & Pope, R. M. Clin. Immun. Immunopatn. 69, 29–35 (1993).

  16. 16

    Carson, C. W., Beall, L. D., Hunder, G. G., Johnson, C. M. & Newman, W. J. Rheumatol. 21, 605–611 (1994).

  17. 17

    Koch, A. E. et al. J. Immun. 152, 149–152 (1994).

  18. 18

    Klagsbrun, M. & D'Amore, P. A. A. Rev. Physiol. 53, 217–239 (1991).

  19. 19

    Leibovich, S. J. et al. Nature 329, 630–632 (1987).

  20. 20

    Bussolino, F. et al. Nature 337, 471–473 (1989).

  21. 21

    Leibovich, S. J., Polverini, P. J., Fong, T. W., Harlow, L. A. & Koch, A. E. Proc. natn. Acad. Sci. U.S.A 91, 4190–4194 (1994).

  22. 22

    Carlos, T. M. et al. Blood 76, 965–970 (1990).

  23. 23

    Osborn, L., Vassolla, C. & Benjamin, C. D. J. exp. Med. 176, 99–107 (1992).

  24. 24

    Pulido, R. et al. J. biol. Chem. 266, 10241–10245 (1991).

  25. 25

    Winer, B. J. Statistical Principles in Experimental Design (McGraw-Hill, New York, 1971).

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Koch, A., Halloran, M., Haskell, C. et al. Angiogenesis mediated by soluble forms of E-selectin and vascular cell adhesion molecule-1. Nature 376, 517–519 (1995) doi:10.1038/376517a0

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