Fluorescence polarization — a new tool for cell and molecular biology

Article metrics

Fluorescence polarization (FP) equilibrium binding assays differ from other types of binding studies in one important regard: they require no steps to separate free from bound tracer and are therefore fast, simple and accurate.

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Perrin, F. J. Phys. Radium 7, 390–401 (1926).

  2. 2

    Dandliker, W. B., Hsu, M. L., Levin, J. & Rao, B. R. Meth. Enzym. 74, 3–28 (1981).

  3. 3

    Jolley, M. E. J. analyt. Tox. 5, 236–240 (1981).

  4. 4

    Wei, A. P. & Herron, J. N. Analyt. Chem. 65, 3372–3377 (1993).

  5. 5

    Radek, J. T., Jeong, J. M., Wilson, J. & Lorand, L. Biochemistry, 32, 3527–3534 (1993).

  6. 6

    LeTilly, V. & Royer, C. A. Biochemistry 32, 7753–7758 (1993).

  7. 7

    Bolger, R. & Checovich, W. BioTechniques 17, 585–589 (1994).

  8. 8

    Maeda, H. Analyt. Biochem. 92, 222–227 (1979).

  9. 9

    Yonemura, K. & Maeda, H. J. Biochem. 92, 1297–1303 (1982).

  10. 10

    Bolger, R. & Thompson, D. Am. biotech. Lab. 12, 113–116 (1994).

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Checovich, W., Bolger, R. & Burke, T. Fluorescence polarization — a new tool for cell and molecular biology. Nature 375, 254–256 (1995) doi:10.1038/375254a0

Download citation

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.