Structural determinants of nuclear receptor assembly on DNA direct repeats

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Abstract

Nuclear receptor heterodimers recognize response elements composed of two direct repeats of the consensus sequence 5′-AGGTCA-3′ separated by one to five base pairs. The 1.9 Å crystal structure of the complex formed by the DNA-binding domains of the 9-cis retinoic acid receptor and thyroid hormone receptor bound to a thyroid-response element shows that the subunits interact through a DNA-supported interface involving the carboxy-terminal extension of the DNA-binding domain of the thyroid hormone receptor. The stereochemistry suggests a mechanism by which heterodimers recognize the inter-half-site spacing between direct repeats.

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Rastinejad, F., Perlmann, T., Evans, R. et al. Structural determinants of nuclear receptor assembly on DNA direct repeats. Nature 375, 203–211 (1995) doi:10.1038/375203a0

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