Abstract
THE Fos and Jun families of eukaryotic transcription factors heterodimerize to form complexes capable of binding 5'-TGAGTCA-3' DNA elements. We have determined the X-ray crystal structure of a heterodimer of the bZIP regions of c-Fos and c-Jun bound to DNA. Both subunits form continuous α-helices. The carboxy-terminal regions form an asymmetric coiled-coil, and the amino-terminal regions make base-specific contacts with DNA in the major groove. Comparison of the two crystallographically distinct protein-DNA complexes show that the coiled-coil is flexibly joined to the basic regions and that the Fos-Jun heterodimer does not recognize the asymmetric 5'-TGAGTCA-3' recognition element in a unique orientation. There is an extensive network of electrostatic interactions between subunits within the coiled-coil, consistent with proposals that these interactions determine preferential formation of the heterodimer over either of the homodimers.
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References
Abate, C., Patel, L., Rauscher III, F. J. & Curran, T. Science 248, 1157–1161 (1990).
Ellenberger, T. E., Brandl, C. J., Struhl, K. & Harrison, S. C. Cell 71, 1223–1237 (1992).
König, P. & Richmond, T. J. J. molec. Biol. 233, 139–154 (1993).
Kerppola, T. K. & Curran, T. Science 254, 1210–1214 (1991).
Nakabeppu, Y. & Nathans, D. EMBO J. 8, 3833–3841 (1989).
Turner, R. & Tjian, R. Science 243, 1689–1694 (1989).
Gentz, R., Rauscher III, F. J., Abate, C. & Curran, T. Science 243, 1695–1699 (1989).
Neuberg, M., Schuermann, M., Hunter, J. B. & Müller, R. Nature 338, 589–590 (1989).
Risse, G., Jooss, K., Neuberg, M., Brüller, H.-J. & Müller, R. EMBO J. 8, 3825–3832 (1989).
Ransone, L. J., Visvader, J., Wamsley, P. & Verma, I. M. Proc. natn. Acad. Sci. U.S.A. 87, 3806–3810 (1990).
Crick, F. H. C. Acta crystallogr. 6, 689–697 (1953).
O'Shea, E. K., Klemm, J. D., Kim, P. S. & Alber, T. Science 254, 539–544 (1991).
McLachlan, A. D. & Stewart, M. J. molec. Biol. 98, 293–304 (1975).
Vinson, C. R., Hai, T. & Boyd, S. M. Genes Dev. 7, 1047–1058 (1993).
O'Shea, E. K., Rutkowski, R. & Kim, P. S. Cell 68, 699–708 (1992).
Ferré-D'Amaré, A. R., Prendergast, G. C., Ziff, E. B. & Burley, S. K. Nature 363, 38–45 (1993).
Miner, J. N. & Yamamoto, K. R. Genes Dev. 6, 2491–2501 (1992).
Stein, B. et al. EMBO J. 12, 3879–3891 (1993).
Wu, H., Moulton, K., Horval, A., Parik, S. & Glass, C. K. Molec. cell. Biol. 14, 2129–2139 (1994).
Jain, J. et al. Nature 365, 352–355 (1993).
CCP4, A Suite of Programs for Protein Crystallography (SERC Collaborative Computing Project No. 4, Daresbury Laboratory, Warrington, UK, 1979).
van Straaten, F., Müller, R., Curran, T., van Beveran, C. & Verma, I. Proc. natn. Acad. Sci. U.S.A. 80, 3183–3187 (1983).
Bohmann, D. et al. Science 238, 1386–1392 (1987).
Abate, C., Luk, D., Gentz, R., Rauscher, F. J. & Curran, T. Proc. natn. Acad. Sci. U.S.A. 87, 1032–1036 (1990).
Studier, F. W. & Moffatt, B. A. J. molec. Biol. 189, 113–130 (1986).
Kabsch, W. J. Appl. Crystallogr. 21, 916–924 (1988).
Jones, T. A., Zou, J.-Y., Cowna, S. W. & Kjeldgaard, M. Acta crystallogr. A47, 110–119 (1991).
Brünger, A. T. X-PLOR (Version 3.1): A System for X-ray Crystallography and NMR (Yale Univ. Press, New Haven, 1992).
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Glover, J., Harrison, S. Crystal structure of the heterodimeric bZIP transcription factor c-Fos–c-Jun bound to DNA. Nature 373, 257–261 (1995). https://doi.org/10.1038/373257a0
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DOI: https://doi.org/10.1038/373257a0
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