Differential production of interferon-γ and interleukin-4 in response to Th1- and Th2-stimulating pathogens by γδ T cells in vivo

Abstract

EXPOSURE to various pathogens can stimulate at least two patterns of cytokine production by CD4-positive T cells1–4. Responses that result in secretion of interferon-γ (IFN-γ), lymphotoxin and interleukin-2 (IL-2) are classified as T-helper-1 (Th1)5,6; CD4+ T-cell production of IL-4, IL-5, IL-9, IL-10 and IL-13 is called a T-helper-2 response (Th2)5,6. Differentiation of CD4+ T cells into either Th1 or Th2 cells is influenced by the cytokine milieu in which the initial antigen priming occurs7–9. Here we use flow cytometry to identify the presence of intracellular cytokines (cytoflow) and analyse T-cell production of IFN-γ and IL-4 from mice infected with Listeria monocytogenes or Nippostrongylus brasiliensis. We show that T cells bearing γδ receptors discriminate early in infection between these two pathogens by producing cytokines associated with the appropriate T-helper response. Our results demonstrate that γδ T cells are involved in establishing primary immune responses.

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Sher, A. & Coffman, R. L. A. Rev. Immun. 10, 385–409 (1992).

  2. 2

    Scott, P. & Kaufmann, S. H. Immun. Today 12, 346–348 (1991).

  3. 3

    Sher, A. et al. Immun. Rev. 127, 183–204 (1992).

  4. 4

    Urban, J. F. Jr et al. Immun. Rev. 127, 205–220 (1992).

  5. 5

    Mosmann, T. R., Cherwinski, H., Bond, M. W., Giedlin, M. A. & Coffman, R. L. J. Immun. 136, 2348–2357 (1986).

  6. 6

    Mosmann, T. R. & Coffman, R. L. A. Rev. Immun. 7, 145–173 (1989).

  7. 7

    Rocken, M., Saurat, J.-H. & Hauser, C. J. Immun. 148, 1031–1036 (1992).

  8. 8

    Hsieh, C.-S., Heimberger, A. B., Gold, J. S., O'Garra, A. & Murphy, K. M. Proc. natn. Acad. Sci. U.S.A. 89, 6065–6069 (1992).

  9. 9

    Seder, R. A., Paul, W. E., Davis, M. M. & Fazekas de St Groth, B. J. exp. Med. 176, 1091–1098 (1992).

  10. 10

    Scott, P., Natovitz, P., Coffman, R. L., Pearce, E. & Sher, A. J. exp. Med. 168, 1675–1684 (1988).

  11. 11

    Yamamura, M. et al. Science 254, 277–279 (1991).

  12. 12

    Hsieh, C.-S. et al. Science 260, 547–549 (1993).

  13. 13

    Svetic, A. et al. J. Immun. 150, 3434–3441 (1993).

  14. 14

    Reiner, S. L., Zheng, S., Wang, Z. E., Stowring, L. & Locksley, R. M. J. exp. Med. 179, 447–456 (1994).

  15. 15

    Havran, W. L. & Allison, J. P. Nature 335, 443–445 (1988).

  16. 16

    Hiromatsu, K. et al. J. exp. Med. 175, 49–56 (1992).

  17. 17

    Mombaerts, P., Arnoldi, J., Russ, F., Tonegawa, S. & Kaufmann, S. H. E. Nature 365, 53–56 (1993).

  18. 18

    Barnes, P. F. et al. J. Infect. Dis. 165, 506–512 (1992).

  19. 19

    Fu, Y.-X. et al. J. Immun. 153, 3101–3114 (1994).

  20. 20

    Coffman, R. L., Seymour, B. W. P., Hudak, S., Jackson, J. & Rennick, D. Science 245, 308–310 (1989).

  21. 21

    Katona, I. M., Urban, J. F. Jr & Finkelman, F. D. J. Immun. 140, 3206–3211 (1988).

  22. 22

    Locksley, R. M. et al. J. exp. Med. 179, 1405–1407 (1994).

  23. 23

    Kelso, A. & Gough, N. M. Proc. natn. Acad. Sci. U.S.A. 85, 9189–9193 (1988).

  24. 24

    Ohga, S., Yoshikai, Y., Takeda, Y., Hiromatsu, K. & Nomoto, K. Eur. J. Immun. 20, 533–538 (1990).

  25. 25

    Schild, H. et al. Cell 76, 29–37 (1994).

  26. 26

    O'Brien, R. L. et al. Cell 57, 667–674 (1989).

  27. 27

    Ensor, J. E. et al. Am. J. Physiol. 266, 967–974 (1994).

  28. 28

    Twomey, B. M., McCallum, S., Isenberg, D. A. & Latchman, D. S. Clin. exp. Immun. 93, 178–183 (1993).

  29. 29

    Moser, R. et al. J. Lab. clin. Med. 122, 567–575 (1993).

  30. 30

    Wen, L. Nature 369, 654–658 (1994).

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Ferrick, D., Schrenzel, M., Mulvania, T. et al. Differential production of interferon-γ and interleukin-4 in response to Th1- and Th2-stimulating pathogens by γδ T cells in vivo. Nature 373, 255–257 (1995). https://doi.org/10.1038/373255a0

Download citation

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.