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Cost of mating in Drosophila melanogaster females is mediated by male accessory gland products


FEMALE Drosophila melanogaster with environmentally1–3 or genetically4 elevated rates of mating die younger than controls. This cost of mating is not attributable to receipt of sperm5. We demonstrate here that seminal fluid products from the main cells of the male accessory gland are responsible for the cost of mating in females, and that increasing exposure to these products increases female death rate. Main-cell products are also involved in elevating the rate of female egg-laying, in reducing female receptivity to further matings and in removing or destroying sperm of previous mates6–12. The cost of mating to females may therefore represent a side-effect of evolutionary conflict between males13.

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  1. Fowler, K. & Partridge, L. Nature 338, 760–761 (1989).

    Article  ADS  Google Scholar 

  2. Patridge, L. & Fowler, K. J. insect Physiol. 36, 419–425 (1990).

    Article  Google Scholar 

  3. Chapman, T. J. Insect Physiol. 38, 223–227 (1992).

    Article  Google Scholar 

  4. Trevitt, S. thesis, Univ. Edinburgh (1989).

  5. Chapman, T., Hutchings, J. & Partridge, L. Proc. R. Soc. B 253, 211–217 (1993).

    Article  ADS  CAS  Google Scholar 

  6. Hihara, F. Zool. Mag. (Tokyo) 90, 307–316 (1981).

    Google Scholar 

  7. Manning, A. An. Behav. 15, 239–250 (1967).

    Article  CAS  Google Scholar 

  8. Scott, D. An. Behav. 35, 142–149 (1987).

    Article  Google Scholar 

  9. Chen, P. et al. Cell 54, 291–298 (1988).

    Article  CAS  Google Scholar 

  10. Aigaki, T., Fleischmann, I., Chen, P. S. & Kubli, E. Neuron 7, 557–563 (1991).

    Article  CAS  Google Scholar 

  11. Harshman, L. G. & Prout, T. Evolution (in the press).

  12. Kalb, J. M., DiBenedetto, A. J. & Wolfner, M. F. Proc. natn. Acad. Sci. U.S.A. 90, 8093–8097 (1993).

    Article  ADS  CAS  Google Scholar 

  13. Trivers, R. L. in Sexual Selection and the Descent of Man (ed. Campbell, B.) 136–179 (Aldine, Chicago, 1972).

    Google Scholar 

  14. Bertram, M. J., Akerkar, G. A., Ard, R. L., Gonzalez, C. & Wolfner, M. F. Mech. Dev. 38, 33–40 (1992).

    Article  CAS  Google Scholar 

  15. Meikle, D. B., Sheehan, K. B., Phillis, D. M. & Richmond, R. C. J. insect Physiol. 36, 93–101 (1990).

    Article  CAS  Google Scholar 

  16. Cavener, D. R. & Maclntyre, R. J. Proc. natn. Acad. Sci. U.S.A. 80, 6286–6288 (1983).

    Article  ADS  CAS  Google Scholar 

  17. Monsma, S. A. & Wolfner, M. F. Genes Dev. 2, 1063–1073 (1988).

    Article  CAS  Google Scholar 

  18. DiBenedetto, H. A., Harada, H. A. & Wolfner, M. F. Devl Biol. 139, 134–148 (1990).

    Article  CAS  Google Scholar 

  19. Ludwig, M. Z. et al. Biochem. Genet. 29, 215–240 (1991).

    Article  CAS  Google Scholar 

  20. Bairati, A. & Perotti, M. E. Drosophila information Service 45, 67–68 (1970).

    Google Scholar 

  21. Partridge, L., Green, A. & Fowler, K. J. Insect Physiol. 33, 745–749 (1987).

    Article  Google Scholar 

  22. Boswell, R. E. & Mahowald, A. P. Cell 43, 97–104 (1985).

    Article  CAS  Google Scholar 

  23. Aguadé, M., Miyashita, N. & Langley, C. H. Genetics 132, 755–770 (1992).

    PubMed  PubMed Central  Google Scholar 

  24. Van Valen, L. M. Evol. Theory 7, 93–106 (1985).

    Google Scholar 

  25. Trevitt, S., Fowler, K. & Partridge, L. J. Insect Physiol. 34, 821–828 (1988).

    Article  Google Scholar 

  26. Chapman, T., Trevitt, S. & Partridge, L. J. evol. Biol. 7, 51–69 (1994).

    Article  Google Scholar 

  27. Dixon, W. J. BMDP Statistical Software Manual Vol. 2 (Berkley Press, California, 1988).

    Google Scholar 

  28. Rice, W. R. Evolution 43, 223–225 (1989).

    Article  Google Scholar 

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Chapman, T., Liddle, L., Kalb, J. et al. Cost of mating in Drosophila melanogaster females is mediated by male accessory gland products. Nature 373, 241–244 (1995).

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