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A polyadenylation factor subunit is the human homologue of theDrosophila suppressor of forked protein

Nature volume 372, pages 471–474 (1994)Cite this article

Abstract

POLYADENYLATION of messenger RNA precursors is a complex process that requires multiple protein factors (for reviews, see refs 1, 2). Cleavage stimulation factor (CstF) is one of these, function-ing together with cleavage-polyadenylation specificity factor, two cleavage factors, and poly(A)+ polymerase3–7. CstF is composed of three subunits of Mr 77, 64 and 50K8,9. The 64K10 and 50K11 subunits contain, respectively, an RNP-type RNA-binding domain that contacts the pre-mRNA and transducin repeats characteristic of G-protein β-subunits. Here we report the cloning and characterization of the 77K subunit of human CstF (referred to as 77K). We show that the 77K subunit is required for formation of active CstF and bridges the 64K and 50K subunits. Sequence analyses indicate that the 77K subunit is the homologue of the protein encoded by the Drosophila melanogaster suppressor of forked (su(f)) gene12. Mutations insu(f) can enhance or suppress the effects of transposable element insertions13, and our data indicate that this is due to changes in polyadenylation. Both the 77K subunit and the su(f) protein share homology with Saccharomyces cerevisiae RNA14, previously shown to be involved in mRNA metabolism14. Our results thus also indicate that components of the complex polyadenylation machinery are conserved from yeast to man.

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Authors and Affiliations

  1. Department of Biological Sciences, Columbia University, New York, New York, 10027, USA

    Yoshio Takagaki & James L. Manley

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  1. Yoshio Takagaki
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  2. James L. Manley
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Takagaki, Y., Manley, J. A polyadenylation factor subunit is the human homologue of theDrosophila suppressor of forked protein. Nature 372, 471–474 (1994). https://doi.org/10.1038/372471a0

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