Abstract
LITTLE is known about host factors necessary for retroviral virion assembly or uncoating. We have previously shown that the principal structural protein of the human immunodeficiency virus HIV-1, the Gag polyprotein, binds the cyclophilin peptidyl–prolyl isomerases1; cyclophilins catalyse a rate-limiting step in protein folding2 and protect cells from heat shock3. Here we demonstrate that cyclophilin A is specifically incorporated into HIV-1 virions but not into virions of other primate immunodeficiency viruses. A proline-rich region conserved in all HIV-1 Gag polyproteins is required for cyclophilin A binding and incorporation. Disruption of a single proline blocks the Gag–cyclophilin interactionin vitro, prevents cyclophilin A incorporation into virions, and inhibits H1V-1 replication. Our results indicate that the interaction of Gag with cyclophilin A is necessary for the formation of infectious HIV-1 virions.
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Franke, E., Yuan, H. & Luban, J. Specific incorporation of cyclophilin A into HIV-1 virions. Nature 372, 359–362 (1994). https://doi.org/10.1038/372359a0
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DOI: https://doi.org/10.1038/372359a0
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