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The ETS domain protein Pointed-P2 is a target of MAP kinase in the Sevenless signal transduction pathway

Nature volume 370, pages 386–389 (1994)Cite this article

Abstract

THE fate of the R7 photoreceptor cell in the developing eye of Drosophila is controlled by the Sevenless (Sev) receptor tyrosine kinase1,2. Sev activates a highly conserved signal transduction cascade involving the proteins Rasl and Raf and the Rolled/mitogen-activated protein (Rl/MAP) kinase3. Here we show that the ETS domain protein encoded by the P2 transcript of the pointed (pnt) gene is a nuclear target of this signalling cascade which acts downstream of Rl/MAP kinase. The PntP2 protein is phosphorylated by Rl/MAP kinase in vitro at a single site and this site is required for its function in vivo. Furthermore, we present genetic and biochemical data suggesting that MAP kinase controls neural development through phosphorylation of two antagonizing transcription factors of the ETS family, Van and PntP2.

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References

  1. Greenwald, I. & Rubin, G. M. Cell 68, 271–281 (1992).

    Article  CAS  Google Scholar 

  2. Dickson, B. & Hafen, E. in The Development of Drosophila (eds Martinez-Arias, A. & Bate, M.) (Cold Spring Harbor Press, New York, 1993).

    Google Scholar 

  3. Dickson, B. & Hafen, E. Curr. Opin. genet. Dev. 4, 64–70 (1994).

    Article  CAS  Google Scholar 

  4. Brunner, D. et al. Cell 76, 875–888 (1994).

    Article  CAS  Google Scholar 

  5. Biggs, W. H. III et al. EMBO J. 13, 1628–1635 (1994).

    Article  CAS  Google Scholar 

  6. Chen, R. H., Sarnecki, C. & Blenis, J. Molec. cell. Biol. 12, 915–927 (1992).

    Article  CAS  Google Scholar 

  7. Lenormand, P. et al. J. Cell Biol. 122, 1079–1088 (1993).

    Article  CAS  Google Scholar 

  8. Treisman, R. Curr. Opin. genet. Dev. 4, 96–101 (1994).

    Article  CAS  Google Scholar 

  9. Marais, R., Wynne, J. & Treisman, R. Cell 73, 381–393 (1993).

    Article  CAS  Google Scholar 

  10. Chen, T., Bunting, M., Karim, F. D. & Thummel, C. S. Devl Biol. 151, 176–191 (1992).

    Article  CAS  Google Scholar 

  11. Lai, Z.-C. & Rubin, G. M. Cell 70, 609–620 (1992).

    Article  CAS  Google Scholar 

  12. Klämbt, C. Development 117, 163–176 (1993).

    PubMed  Google Scholar 

  13. Basler, K. & Hafen, E. Development 107, 723–731 (1989).

    CAS  PubMed  Google Scholar 

  14. Simon, M. A., Bowtell, D., Dodson, G. S., Laverty, T. R. & Rubin, G. M. Cell 67, 701–716 (1991).

    Article  CAS  Google Scholar 

  15. Rogge, R. D., Karlovich, C. A. & Banerjee, U. Cell 64, 39–48 (1991).

    Article  CAS  Google Scholar 

  16. Dickson, B., Sprenger, F., Morrison, D. & Hafen, E. Nature 360, 600–603 (1992).

    Article  ADS  CAS  Google Scholar 

  17. Basler, K., Siegrist, P. & Hafen, E. EMBO J. 8, 2381–2386 (1989).

    Article  CAS  Google Scholar 

  18. Janknecht, R., Ernst, W. H., Pingoud, V. & Nordheim, A. EMBO J. 12, 5097–5104 (1993).

    Article  CAS  Google Scholar 

  19. Marshall, C. J. Curr. Opin. genet. Dev. 4, 82–89 (1994).

    Article  CAS  Google Scholar 

  20. Wasylyk, B., Hahn, S. L. & Giovane, A. Eur. J. Biochem. 211, 7–18 (1993).

    Article  CAS  Google Scholar 

  21. Jürgens, G. E. W., Nüsslein-Volhard, C. & Kluding, H. Wilhelm Roux Arch. dev. Biol. 193, 183–295 (1984).

    Article  Google Scholar 

  22. Mayer, U. & Nüssleln-Volhard, C. Genes Dev. 2, 1496–1511 (1988).

    Article  CAS  Google Scholar 

  23. Scholz, H., Deatrick, J., Klaes, A. & Klämbt, C. Genetics 135, 455–468 (1993).

    CAS  PubMed  PubMed Central  Google Scholar 

  24. Tautz, D. & Pfeifle, C. Chromasoma 98, 81–85 (1989).

    Article  CAS  Google Scholar 

  25. Gaul, U., Mardon, G. & Rubin, G. M. Cell 68, 1007–1019 (1992).

    Article  CAS  Google Scholar 

  26. Brand, A. H. & Perrimon, N. Development 118, 401–415 (1993).

    CAS  Google Scholar 

  27. Basler, K., Yen, D., Tomlinson, A. & Hafen, E. Genes Dev. 4, 728–739 (1990).

    Article  CAS  Google Scholar 

  28. Basler, K., Christen, B. & Hafen, E. Cell 64, 1069–1082 (1991).

    Article  CAS  Google Scholar 

  29. Alessi, D. R. et al. EMBO J. 13, 1610–1619 (1994).

    Article  CAS  Google Scholar 

  30. Traverse, S., Gomez, N., Paterson, H., Marshall, C. & Cohen, P. Biochem. J. 288, 351–355 (1992).

    Article  CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Zoologisches Institut, Universitat Zurich, Winterthurerstrasse 190, CH-8057, Zurich, Switzerland

    Damian Brunner, Klaus Dücker, Nadja Oellers & Ernst Hafen

  2. Institut fur Entwickiungsbiologie, Universitat Koln, D-50923, Koln, Germany

    Henrike Scholzi & Christian Klambt

Authors
  1. Damian Brunner
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  2. Klaus Dücker
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  3. Nadja Oellers
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  4. Ernst Hafen
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  5. Henrike Scholzi
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  6. Christian Klambt
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Brunner, D., Dücker, K., Oellers, N. et al. The ETS domain protein Pointed-P2 is a target of MAP kinase in the Sevenless signal transduction pathway. Nature 370, 386–389 (1994). https://doi.org/10.1038/370386a0

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