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Nuclear protein CBP is a coactivator for the transcription factor CREB

Nature volume 370, pages 223–226 (1994)Cite this article

Abstract

THE transcription factor CREB binds to a DNA element known as the cAMP-regulated enhancer (CRE)1–5. CREB is activated through phosphorylation by protein kinase A (PKA)6, but precisely how phosphorylation stimulates CREB function is unknown. One model is that phosphorylation may allow the recruitment of coactivators which then interact with basal transcription factors. We have previously identified a nuclear protein of Mr 265K, CBP, that binds specifically to the PKA-phosphorylated form of CREB7. We have used fluorescence anisotropy measurements to define the equi-librium binding parameters of the phosphoCREB:CBP interaction and report here that CBP can activate transcription through a region in its carboxy terminus. The activation domain of CBP interacts with the basal transcription factor TFIIB through a domain that is conserved in the yeast coactivator ADA-1 (ref. 8). Consistent with its role as a coactivator, CBP augments the activ-ity of phosphorylated CREB to activate transcription of cAMP-responsive genes.

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Affiliations

  1. Vollum Institute and Department of Biochemistry, Oregon Health Sciences University, Portland, Oregon, 97201, USA

    Roland P. S. Kwok, James R. Lundblad, John C. Chrivia, Jane P. Richards, Hans Peter Bächinger, Richard G. Brennan, Stefan G. E. Roberts, Michael R. Green & Richard H. Goodman

  2. Department of Pharmacological and Physiological Sciences, St Louis University, St Louis, Missouri, 63014, USA

    John C. Chrivia

  3. Shriner's Hospital, Portland, Oregon, 97201, USA

    Hans Peter Bächinger

  4. Program in Molecular Medicine, University of Medical Center, Worcester, Massachusetts, 01605, USA

    Stefan G. E. Roberts & Michael R. Green

Authors
  1. Roland P. S. Kwok
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  2. James R. Lundblad
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  3. John C. Chrivia
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  4. Jane P. Richards
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  5. Hans Peter Bächinger
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  6. Richard G. Brennan
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  7. Stefan G. E. Roberts
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  8. Michael R. Green
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  9. Richard H. Goodman
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Kwok, R., Lundblad, J., Chrivia, J. et al. Nuclear protein CBP is a coactivator for the transcription factor CREB. Nature 370, 223–226 (1994). https://doi.org/10.1038/370223a0

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