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Parthenogenetic activation of oocytes in c-mos-deficient mice

Nature volume 370, pages 68–71 (1994)Cite this article

An Erratum to this article was published on 04 August 1994

Abstract

IN Xenopus the c-mos proto-oncogene product (Mos) is essential for the initiation of oocyte maturation1, for the progression from meiosis I to meiosis II2,3 and for the second meiotic metaphase arrest, acting as an essential component of the cytostatic factor CSF4,5. Its function in mouse oocytes is unclear6–9, however, as is the biological significance of c-mos mRNA expression in testes1,10 and several somatic tissues1,10,11. We have generated c-mos-deficient mice by gene targeting in embryonic stem cells. These mice grew at the same rate as their wild-type counterparts and reproduction was normal in the males, but the fertility of the females was very low. The c-mos-deficient female mice developed ovarian teratomas at a high frequency. Oocytes from these females matured to the second meiotic metaphase both in vivo and in vitro, but were activated without fertilization. The results indicate that in mice Mos plays a role in the second meiotic metaphase arrest, but does not seem to be essential for the initiation of oocyte maturation, spermatogenesis or somatic cell cycle.

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Author notes

  1. Yoji Ikawatll

    Present address: Department of Biochemistry, Tokyo Medical and Dental University School of Medicine, Tokyo, 113, Japan

  2. Nobumoto Watanabe

    Present address: Molecular Biology and Virology Laboratory, The Salk Institute for Biological Studies, PO Box 85800, San Diego, CA, 92186-5800, USA

  3. Yasuhide Furuta, Naoki Takedat & Shinichi Aizawai

    Present address: Laboratory of Morphogenesis, Institute of Molecular Embryology and Genetics, Kumamoto University School of Medicine, 2-2-1 Honjo, Kumamoto, 860, Japan

  4. Hiroyuki Tamemoto

    Present address: The Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Bunkyo, Tokyo, 113, Japan

Authors and Affiliations

  1. Mitsubishi Kasei Institute of Life Sciences, 11 Minamiooya, Machida-shi, Tokyo, 194, Japan

    Naohiro Hashimoto & Minesuke Yokoyama

  2. Tsukuba Life Science Center, The Institute of Physical and Chemical Research (RIKEN), 3-1-1 Koyadai, Tsukuba, lbaraki, 305, Japan

    Nobumoto Watanabe, Yasuhide Furuta, Hiroyuki Tamemoto, Mariko Nagayoshi, Naoki Takedat & Yoji Ikawatll

  3. Division of Molecular Genetics, Institute of Life Science, Kurume University, 2432-3 Aikawa, Kurume, Fukuoka, 830, Japan

    Noriyuiki Sagata & Kenji Okazaki

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  1. Naohiro Hashimoto
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Hashimoto, N., Watanabe, N., Furuta, Y. et al. Parthenogenetic activation of oocytes in c-mos-deficient mice. Nature 370, 68–71 (1994). https://doi.org/10.1038/370068a0

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