Increased glycolysis is characteristic of malignancy. Previously, with a mitochondrial inhibitor, we demonstrated that glycolytic ATP production was sufficient to support migration of melanoma cells. Recently, we found that glycolytic enzymes were abundant and some were increased in pseudopodia formed by U87 glioma (astrocytoma) cells. In this study, we examined cell migration, adhesion (a step in migration), and Matrigel invasion of U87 and LN229 glioma cells when their mitochondria were inhibited with sodium azide or limited by 1% O2. Cell migration, adhesion, and invasion were comparable, with and without mitochondrial inhibition. Upon discovering that glycolysis alone can support glioma cell migration, unique features of glucose metabolism in astrocytic cells were investigated. The ability of astrocytic cells to remove lactate, the inhibitor of glycolysis, via gluconeogenesis and incorporation into glycogen led to consideration of supportive genetic mutations. Loss of phosphatase and tensin homolog (PTEN) releases glycogenesis from constitutive inhibition by glycogen synthase kinase-3 (GSK3). We hypothesize that glycolysis in gliomas can support invasive migration, especially when aided by loss of PTEN's regulation on the phosphatidylinositol-3 kinase (PI3K)/Akt pathway leading to inhibition of GSK3. Migration of PTEN-mutated U87 cells was studied for release of extracellular lactic acid and support by gluconeogenesis, loss of PTEN, and active PI3K. Lactic acid levels plateaued and phosphorylation changes confirmed activation of the PI3K/Akt pathway and glycogen synthase when cells relied only on glycolysis. Glycolytic U87 cell migration and phosphorylation of GSK3 were inhibited by PTEN transfection. Glycolytic migration was also suppressed by inhibiting PI3K and gluconeogenesis with wortmannin and metformin, respectively. These findings confirm that glycolytic glioma cells can migrate invasively and that the loss of PTEN is supportive, with activated glycogenic potential included among the relevant downstream effects.
Malignant glioma (astrocytoma), the most resistant type of brain tumor to treatment, is characterized by diffuse invasiveness that prevents successful resection.1 Earlier studies showed that melanoma cells have the potential to migrate using ATP produced only by glycolysis.2 Increased ATP production via glycolysis is required by tumor cells whenever tissue levels of oxygen fall or mitochondrial respiration is defective,3, 4, 5 especially during active invasion. The resilience of malignant gliomas to anaerobic conditions suggests that their potential for glycolysis is increased.5, 6, 7 Large amounts of glucose can be transported into glioma cells for glycolysis and increased glucose extraction within the tumors8 is accommodated by increased expression of GLUT3 transporters.9, 10, 11 Maintaining increased levels of hypoxia-inducible factor-1α (HIF-1α) is one mechanism that induces glycolytic enzyme expression.12
Recently, in proteomic studies of U87 glioma cells that harbor mutations of phosphatase and tensin homolog (PTEN), we showed prominence of all seven enzymes in the distal, energy producing portion of the glycolytic pathway with enhancement in pseudopodia.13 Cell protrusion, adhesion, deadhesion, and cytoplasmic retraction are steps in the migratory process. Agents that upregulate glycolysis in malignant cells, such as HIF-1α, have already been established as treatment targets in numerous studies. Although using NADH to reduce pyruvate to lactate via lactate dehydrogenase maintains glycolysis by regenerating NAD under anaerobic conditions, the buildup of lactic acid is inhibitory and is usually avoided by rapid release into the bloodstream. Extracellular lactate dehydrogenase to reconvert released lactate back to pyruvate would not be available unless nearby cells were injured. The intracellular removal of lactic acid by conversion to glucose and glycogen is a mechanism of metabolic control that has not been explored in tumors. Anaerobic production of lactic acid requires its conversion to prevent inhibition of glycolysis when cells are disconnected from the vasculature and cannot release their lactic acid into the bloodstream.
Metabolic conversion of lactate in astrocytes led to consideration of a potential role for this process in gliomas. Astrocytic cells are unique in that they use glycolysis to produce lactate to be shuttled into nearby neurons and converted into substrate for oxidative metabolism to augment neuronal energy supplies.14, 15, 16, 17, 18 Excess astrocytic lactic acid is either removed via the vasculature or temporarily stored by metabolic conversion into glucose and glycogen or into alanine.14, 19, 20, 21 Signal transduction that permits activation of glycogen synthase (GS).22, 23, 24 aids lactic acid conversion to glycogen in cells with gluconeogenic potential, including astrocytic cells.19, 20 We hypothesize that when negative control of GS is lost due to mutations in gliomas, conversion of lactate into glucose and glycogen allows glycolysis in malignant cells to exceed normal limits. Intermittent rather than continuous vascular contact during tumor invasion may suffice for cells with intracellular lactate removal. Also, tolerance for hypoxia via gluconeogenesis and glycogenesis may support glycolysis in pseudopodia with oxidative metabolism occurring in another cell region to compensate for the energy debt of gluconeogenesis. Glycogen accumulation would vary due to concurrent glycogenolysis.
Mutational loss of normal inhibitory controls on glycolysis that allows tumor cells to escape a strict reliance on vascular support should promote tumor invasion. Mutations of PTEN that commonly occur in gliomas25, 26, 27, 28 lead to loss of negative regulation on the phosphatidylinositol-3 kinase (PI3K)/Akt pathway. The resulting deactivation (phosphorylation) of GS kinase-3 (GSK3) permits activation (dephosphorylation) of GS whenever phosphatases are active. When the pathway is stimulated, inactivated GSK3 is unable to cooperate with other kinases to constitutively inhibit GS. In this study, we investigated the glycolytic potential of U87 and LN229 glioma cells during adhesion and migration through matrix-coated porous filters. The ability of glycolysis to support proliferation in PTEN-mutated cells was also examined in clonogenic assays. Lactic acid levels, phosphorylation changes in Akt, GSK3, and GS, and reliance of cell migration on PTEN, PI3K, and gluconeogenesis, were determined for glycolytic U87 cells that we propose benefit from their mutational loss of PTEN in avoiding lactic acidosis.
Materials and methods
Materials and Cell Culture
Chemicals and reagents, unless otherwise stated, were obtained from Sigma, St Louis, MO, USA. Human U87 MG and LN229 glioma (astrocytoma) cells (American Type Culture Collection, Rockville, MD, USA), were maintained in minimal essential media (MEM) Eagle or alpha-MEM (Cellgro, Mediatech, Herndon, VA, USA) with 10% fetal bovine serum (FBS) (Invitrogen, Carlsbad, CA, USA).
The effects of hypoxia on energy production were generated by either lowering oxygen levels or by inhibiting mitochondria respiration with sodium azide (NaN3) (chemical hypoxia). The concentration of NaN3 was 27 mM except when indicated in a dose curve. Selective inhibition of cytochrome oxidase with NaN3 allowed specific inhibition of aerobic energy metabolism with molecular oxygen remaining available to cellular enzymes involved in synthesis of lipid, collagen, and amino acids, fatty acid oxidation, etc. Previously, NaN3 (5–10 mM) was used to inhibit oxidative energy production in cultured astrocytes14, 29, 30 and systemically in animals.31, 32, 33, 34, 35 Other recent studies using cell culture and tissue-based assays have also relied on NaN3 to generate chemical hypoxia.36, 37 In addition to cytochrome oxidase, NaN3 also inhibits nucleoside triphosphate diphosphohydrolase, an ecto-diphosphohydralase that hydrolyzes nucleosides extracellularly at pH 8.38 Inflammatory cells with catalase and myeloperoxidase can oxidize NaN3 to generate nitric oxide.39 Effects of NaN3 have been shown to be independent of multiple tyrosine kinases.40 A glucose analogue, 2-deoxyglucose (2DG), inhibited glucose metabolism. Once 2DG is transported into cells and phosphorylated, it is trapped intracellularly and inhibits hexokinase.41, 42, 43 Glycogenolysis of glycogen with incorporated 2DG is also inhibited.42, 44 Metformin (MF), or 1,1-dimethylbiguanide, a clinical drug that inhibits gluconeogenesis,45, 46 was dissolved in phosphate-buffered saline (PBS) for testing in migration assays. Wortmannin (Wm), an inhibitor of PI3K,47 was dissolved in dimethylsulfoxide as a 10 mM stock solution for testing in adhesion and migration assays. The glycolytic migration of LN229 cells, lacking mutations of PTEN, was not evaluated with Wm and MF.
Cell Migration Assays
Confluent cells were trypsinized on the day of assay and allowed to recover in media with 10% FBS for 2 h at 37°C. Cells were centrifuged (<500 rpm, <5 min) and resuspended at 1.5 × 106/ml with minimal pipetting in media for migration. For most migration assays U87 cells were suspended in ‘motility media’ (PBS with 0.027% CaCl2·2H2O, 0.02% MgCl2·6H2O, 1% FBS, 0.1% bovine serum albumin (BSA), 5 mM sodium pyruvate, and 1 mg/ml glucose). MEM, with 1% FBS and 0.1% BSA, was used instead of ‘motility media’ in assays of LN229 cells and transfected U87 cells to achieve significant levels of spontaneous migration. The 48-well modified Boyden chambers (Neuro Probe, Gaithersburg, MD, USA) were assembled with porous (8 μm), polycarbonate filters (Neuro Probe), coated with 0.01% porcine gelatin. Cell suspensions were added to upper wells and chambers were incubated at 37°C for 4.5–5 h in 5% CO2 in a routine tissue culture incubator or in an atmosphere containing 1% O2 and 5% CO2. Hypoxia (1% O2), was maintained by a PROOX oxygen monitor (Model 110, BioSpherix, Redfield, NY, USA), within a chamber (BioSpherix) placed in a tissue culture incubator. Upon completion of the assays, filters were stained with Diff Quik (Allegiance, McGaw Park, IL, USA) and analyzed (multiple replicates per data point). Migrated cell nuclei were counted or migrated cells were digitized using a transparency scanner (Epson Perfection 2450 PHOTO, Epson America, Long Beach, CA, USA), and densitometry (UN-SCAN-It gel, Silk Scientific, Orem, UT, USA) with correction for background filter density. The two methods of quantifying cell migration (nuclear counts vs scanned cells) showed significant correlation, r2=0.9. Assays were considered adequate for quantification when densities of migrated control cells from each well were at least several times greater than background on each filter and at least several hundred (or more) control cell nuclei migrated per chamber well. Viability of ‘resting’ cells (rocked at room temperature), with and without exposure to inhibitors, was tested with Trypan blue exclusion to rule out direct toxic effects on cells during the assays.
Adhesion and deadhesion are steps in the process of migration. In separate assays, suspensions (1 × 106/ml) of U87 and LN229 cells were added to 0.1% gelatin-coated multiwell plastic Costar plates (Corning, Corning, NY, USA). PBS-based motility media was used for U87 cells except for comparison assays with LN229 cells, when MEM was used for both cell types. After 2 h incubations at 37°C, the plates were washed twice with PBS and stained with Diff Quik. Adherent cells were scanned and analyzed with densitometry.
U87 (100 000/ml) and LN229 (200 000/ml) cells, suspended in MEM with 0.1% BSA and the fluorescent dye, DiI (Molecular Probes, Invitrogen), 1 μg/ml, were loaded in 24-well Matrigel Invasion Chambers (BD Biosciences), with and without 5% FBS and NaN3. A motogenic agent, hepatocyte growth factor (HGF), 2.5 ng/ml, was also added to the bottom wells of plates loaded with LN229 cells to optimize their migration. The Matrigel-coated plates were incubated for 24 h at 37°C in routine tissue culture conditions. In each well, the fluorescence emitted from the bottom filter surface at 595 nm in response to excitation at 540 nm was quantified in a fluorescent plate reader, HTS 7000 Plus Bio Assay Reader and HTFSoft 2.0 software (Perkin Elmer, Shelton, CT, USA). Invaded cells were visualized to correlate with fluorescent results with an Axiovert S100 Fluorescent Microscope and were photographed using an AxioCam CCD camera controlled with KS300 software (Zeiss, Goettingen, Germany). Each replicate was corrected for background fluorescence of bottom wells with labeled cells loaded in the top inserts but with no HGF or serum in the bottom wells. Background fluorescence was the same as seen when no labeled cells were added.
Solutions of U87 cells were treated with NaN3, both NaN3 and 2DG (2 mg/ml), or no inhibitors for 5 h (length of migration assays) or overnight in 1% FBS-MEM with 0.1% BSA and then plated in 10% FBS-MEM. The cells, 250 per well, loaded in six-well plates were stained on the 9th day of culture and inspected to determine the number of visible clones formed.
Replication-deficient adenoviral (Ad) expression vectors were used to restore PTEN-wild type (WT) to U87 cells. Adenoviruses with PTEN-WT and control adenovirus were constructed according to Vogelstein and colleagues.48, 49 Briefly, PTEN (a kind gift from Dr Kenneth Yamada, NIH) was subcloned into the pAdTrack shuttle vector also containing a green fluorescent protein (GFP) reporter transgene. pAdTrack was cotransfected with pAdEasy viral DNA into BJ5183 bacteria for homologous recombination. Recombinants were then transfected into 293 embryonic kidney cells for production of adenoviruses that were designated Ad-PTEN and Ad-control, respectively. Viral titers, as determined by plaque-formation assays, were approximately 1010 pfu/ml. Transient transfections were accomplished by infecting the cells with Ad-PTEN or Ad-control (multiplicity of infection=10), 1.6–3.2 × 106 pfu/ml added. After 24–48 h, cells were viewed with a fluorescent microscope, BH2-RFCA (Olympus, Lake Success, NY, USA) to evaluate GFP as an indicator of transfection in the cells.
Lactic Acid Assays
Conditioned media was aspirated from the upper Boyden chamber wells at the end of the motility assays and from flasks of cells transfected with Ad-PTEN and Ad-control in separate assays. Replicates were assayed for lactic acid content using Lactate Reagent (Sigma), according to the manufacturer's instructions. Lactic acid was measured after conversion to pyruvate and hydrogen peroxide by lactate oxidase. In hydrogen peroxide, peroxidase catalyzed the oxidative condensation of chromogen precursors to produce a dye whose absorbance at 540 nm was directly proportional to concentrations of lactate in the samples.
Alterations in Phosphorylation of Akt, GSK, and GS in Glioblastoma Cells
Whole cell lysates containing 0.5% Igepal, a nonionic detergent, were prepared from U87 and LN229 cells. The U87 cells were incubated in motility media (with and without NaN3) for 4.5–5 h in studies to examine the results of mitochondrial inhibition that forced cell reliance on glycolysis. The transfected U87 cells (Ad-PTEN and Ad-control) were incubated in 1% FBS-MEM for 5 h. Lysates were electrophoresed in 10% polyacrylamide gels under reducing conditions and then transferred onto polyvinylidene difluoride membranes (Invitrogen) for immunoblotting. The amounts of total protein loaded in each lane were 10 μg for all studies except 6 μg was used for GSK3α. The membranes were blocked (Detector Block, Protein Detector Western Blot Kit LumiGLO System, Kirkegaard & Perry Laboratories, Gaithersburg, MD, USA) and reacted with anti-Akt1 (1 μg/ml), anti-phospho(p)-Akt (Thr 308) (1:500), anti-pGSK3α (Ser 21) (2 μg/ml) (Upstate Inc., Lake Placid, NY, USA), anti-GS (1:1000) (Chemicon, Temecula, CA, USA), anti-Akt1, anti-pAkt1 (Ser 473), anti-GSK3β, anti-pGSK3β (Ser 9), anti-GSK3α (Ser 21), anti-GSK3α/β (Ser 21/9), anti-pGS (Ser 640) (1:1000) (Cell Signaling), and anti-actin (1:500) (Sigma). The secondary antibodies, 1:5000 (Kirkegaard & Perry Laboratories) were horseradish peroxidase (HRP)-labeled anti-sheep for some reactions with anti-Akt1 and anti-pGSK3α (Ser 21), HRP-anti-rabbit for anti-pAkt (Thr 308), anti-pAkt (Ser 473), anti-pGSK3β (Ser 9), and anti-pGS (Ser 640), and anti-actin, and HRP-anti-mouse for anti-GS and anti-pGSK3α (Ser 21). A rabbit secondary antibody (Jackson ImmunoResearch, West Grove, PA, USA) was also used for anti-Akt, anti-pAkt (Ser 473), and anti-pGSK3α/β (Ser 21/9). Bands were visualized with HRP conversion of a luminol-based solution to produce chemiluminescence, digitized, and analyzed. Band densities were corrected for adjacent background.
RNA Message Expression of GS and Hypoxia Genes with Mitochondrial Inhibition
Transcriptional regulation of GS was compared to known hypoxia genes during the time span used for migration assays to determine the contribution of transcriptional control to glycogenesis. U87 cells were incubated in PBS-based motility media (with and without NaN3) for 4.5 h (length of motility assays). Cellular RNA was isolated (RNeasy kit, Qiagen, Valencia, CA USA or TRIzol Reagent, Invitrogen). Biotinylated cDNA probes were prepared from the RNA for use on microarray blots (SuperArray Signal Transduction Pathway Finder GEArray Q Series kit, SuperArray Inc., Bethesda, MD, USA) according to the manufacturer's instructions. In all, 96 genes associated with signal transduction were available for comparison of cellular RNA expression, with and without NaN3 present. Three pairs of microarray blots were probed, scanned, and digitized to quantify message signals with densitometry. Message densities were corrected for adjacent background and normalized to cyclophilin A.
Means with 95% confidence intervals (CIs) are stated. Differences between control cells and individual doses of inhibitors or PTEN-WT transfection were tested with two-tailed Student's t-tests after converting each data point to the percent of average control cell value for each assay to place all values on the same scale. The expression of results as a percent of the average control helped to control for assay to assay variability. Comparisons of control cells with multiple doses of inhibitors were tested with ANOVA (S-Plus, SPSS), using the numerical values of each data point (scanned pixels or migrated nuclei). Scanned results were corrected for background. ANOVA was also used to detect synergistic effects of multiple treatments or conditions. Variables typically included assay, inhibitor concentration, and the presence or absence of hypoxia.
U87 and LN229 Cell Migration with Metabolic Inhibition
The glycolytic potential of U87 and LN229 cells was demonstrated in 4.5–5 h migration assays. Both cell types demonstrated migration under normal conditions that persisted when cells were forced to rely on glycolysis by adding a mitochondrial inhibitor, NaN3. A representative assay of U87 cells in PBS-based motility media demonstrated control migration (no inhibitors) with substantial metabolic reserves, as indicated by comparable levels of glycolytic migration (NaN3 inhibition of mitochondria) and hypoglycemic migration (two-fold greater amounts of 2DG present than media glucose) (Figure 1a). However, cell migration was significantly (P<0.00001) vulnerable when both metabolic inhibitors were present as shown in the averaged results of three assays (Figure 1b). Comparisons with ANOVA confirmed significant (P<0.0001) synergy between NaN3 and 2DG for inhibiting U87 migration. Levels of lactate produced in three migration assays of U87 cells exposed to 2DG were significantly lower (P-value <0.02) than the levels produced by control cells (no inhibitors). In these assays, lactate levels produced by migrated control cells and cells exposed to NaN3 were 4.03 (95% CI=3.2–4.86) and 5.07 (95% CI=4.43–5.71) mmol/l, respectively, (Figure 1c). Comparisons with ANOVA for lactate production showed a significant (P<0.001) effect of 2DG, a trend for synergistic effects of 2DG and NaN3 (P=0.056), and no effect for NaN3 alone (P=0.306).
In three representative assays, the migration of LN229 cells in the presence of NaN3 was reduced (P<0.0001), but only to 74.3% (95% CI=65.9–82.7%) of control levels. Migration in 2DG, alone and combined with NaN3, was significantly reduced (P<0.00001) (Figure 1d). There was no significant (P=0.28) difference in the amounts of lactic acid released into the media by LN229 cells exposed to NaN3 compared to levels for control cells. Lactic acid released from LN229 cells exposed to 2DG, with and without NaN3, was significantly reduced (P<0.00001) compared to levels released by control cells (not shown).
U87 and LN229 Cell Adhesion with only Glycolytic ATP Available
Cell adhesion and deadhesion are important steps in the migration process. In MEM, both U87 and LN229 cells maintained significant levels of glycolytic adhesion but U87 cells demonstrated increased sensitivity to 2DG compared to control adhesion (no inhibitors). U87 adhesion with separate exposures to 2DG and NaN3 were 78% (95% CI=73–83%) and 98% (95% CI=94–102%), respectively, of control levels. Levels of LN229 adhesion with separate exposures to 2DG and NaN3 were 105% (95% CI=102–108%) and 89% (95% CI=84–94%), respectively, of control levels. Levels of U87 and LN229 adhesion with combined exposure to 2DG, 1 mg/ml, and NaN3 were 1% (95% CI=0–2%) and 22% (95% CI=9–35%), respectively, of control adhesion for each cell line (Figure 2). Comparisons with ANOVA showed significant synergy for both inhibitors on the cells (P<0.0001). Significant differences (P<0.01) occurred between the two cell types for exposure to 2DG (each concentration) with t-tests and for all doses in chemical hypoxia using ANOVA (P<0.02).
Invasive Migration, with and without Mitochondrial Inhibition
Assays of 24 h cellular invasion through filter pores occluded by Matrigel, a basement membrane material, provided a more rigorous (longer) evaluation of the glycolytic potential in U87 and LN229 cells. Fluorescence of invaded U87 cells that relied on glycolysis (NaN3 present) in a chemoattractant 0–5% gradient of FBS was comparable to control invasion (no inhibitors) fluorescence. Chemokinetic invasion (5% FBS in top and bottom wells) by glycolytic cells was 79.1% (95% CI=69.7–88.5%) of chemoattractant control (no NaN3) levels. Invasion against a reversed (5–0%) gradient of FBS was limited to 15.7% (95% CI=10.6–20.8%) of chemoattractant invasion and was not evaluated for changes when mitochondria were inhibited (Figure 3a). Glycolytic invasion with 1 and 2 mg/dl 2DG was reduced to 59.3 (95% CI=52.3–66.3%) and 17.3% (95% CI=3.9–30.7%), respectively, of control invasion (no inhibitors and 0–5% FBS gradient) (Figure 3b). Glycolytic invaded cells (NaN3 present) uniformly exhibited rounded shapes. Whereas, most invasive cells without NaN3 present were also rounded, a few cells did spread out as shown (Figure 3a, lower panels). HGF-stimulated LN229 cells, with their mitochondria inhibited by NaN3, demonstrated 108.3% (95% CI=94.5–122.1%) and 109.9% (95% CI=96.5–122.9%) levels of control (no NaN3 and 0–5% FBS gradient) invasion for chemoattraction and chemokinesis, respectively, in two assays (not shown).
Migration and Release of Lactic Acid by U87 Cells in 1% O2
Cell migration occurred in true hypoxia (1% O2), with and without mitochondrial inhibition. U87 cells retained migration in PBS-based migration media in 1% O2, without or with 10–50 mM NaN3 present. For each dose of NaN3, with normal or 1% O2, cell migration either remained similar to control migration (normoxia without NaN3) or increased (P<0.04) up to 129.4% (95% CI=108.4–150.4%) (Figure 4a). Levels of extracellular lactic acid released by cells exposed to 10–50 mM NaN3 in normal or 1% O2 were elevated in a small plateau (P<0.05) with the highest value at 119.7% (95% CI=113.0–126.4%) of the average control value (Figure 4b).
Clonogenic Potential of Glycolytic U87 Cells
Retention of potential for proliferation following hypoxic migration of invasive tumor cells would correlate with the malignant behavior of glioblastomas seen clinically. In three assays, cells treated with NaN3 for 5 h prior to plating produced 82% (95% CI=58–106%) of the number of clones produced by untreated cells after 9 days. Overnight (18 h) exposure to NaN3 and 2DG was required to abolish the clonogenic potential in all three assays with results at 0.3% (95% CI=0–0.8%) of control levels (not shown).
Phosphorylation of Akt, GSK3, and GS
Lysates of glioblastoma cells (U87 or LN229) plated for 5 h were analyzed on immunoblots reacted with anti-Akt1, anti-pAkt 1 (Thr 308), anti-pAkt 1 (Ser 473), anti-pGSK3α (Ser 21), anti-GSK3β, anti-pGSK3β (Ser 9), anti-pGSK3α/β (Ser 21/Ser 9), anti-actin, anti-GS, and anti-pGS (Ser 640). Cell lysate loading was equalized for total protein with 10 μg per lane. The effects of chemical hypoxia on the deregulated PI3K/Akt pathway were studied in U87 cells with mutated PTEN. When paired sets of U87 cells were cultured, with and without NaN3, the phosphorylation of Akt 1 (Thr 308) and GSK3α (Ser 21) increased 3.3 × and 2.9 × , respectively, in the presence of the mitochondrial inhibitor that forced the cells to be glycolytic. A slight increase (1.3 × ) in total Akt1 was present in the NaN3-exposed cells. Concentrations of actin remained the same. Phosphorylation of GS in glycolytic cells was reduced down to 12.3% of the control level (cells without NaN3), indicating increased enzyme activity when the mitochondria were inhibited (Figure 5). A moderate reduction in GS phosphorylation, 65.7% of the normal level, was seen in 1% O2 (not shown). In the mutated PTEN cell line, U87, the level of GS was 4.63X greater compared to LN229 cells with intact PTEN. The increase (2.29 × ) in the phosphorylated (inactive) form of GS was only half as much increased in U87 cells. Therefore, a larger fraction and absolute amount of GS was active in U87 cells under normal conditions. Correspondingly, the levels of GSK3β and pGSK3β were 5.88X and 11.6 × greater, respectively, in U87 cells than in LN229 cells (not shown).
Lack of Transcriptional Regulation on GS in U87 Cells Responding to Mitochondrial Inhibition
The potential contribution of transcriptional regulation of GS activity induced by mitochondrial inhibition for 4.5 h was evaluated among 96 signal transduction-related genes. Messages for known hypoxia-associated proteins, early growth response-1 (egr-1) and fos,50, 51, 52, 53, 54 were included. Whereas, message levels for glycogen synthase did not demonstrate significant changes, messages for fos and egr-1, 0 and 16.8%, respectively, of a control message (cyclophilin A), increased to 46.3 and 42.4%, respectively, in U87 cells (Figure 6).
Inhibition of PI3K by Wortmannin (Wm), with and without Mitochondrial Inhibition
Wm, a noncompetitive inhibitor of PI3K, was used to examine the effect of PI3K/Akt pathway inhibition on U87 cell migration and adhesion in PBS-based motility media. Wm inhibited spontaneous motility and adhesion under normal conditions and when cells were forced to be glycolytic by inhibiting mitochondria with NaN3. At each concentration of Wm, 10 nM and greater, significant inhibition occurred for both cell functions, with or without mitochondrial inhibition. In comparison to controls (average cell function with no inhibitors), 30 nM Wm inhibited glycolytic cell migration down to 8.5% (95% CI=4.5–12.5%) (Figure 7a) and cell adhesion down to 33.4% (95% CI=26.9–39.9%) (Figure 7b). Cell migration differed significantly from control migration for all Wm concentrations without NaN3 exposure (P<0.02), and at 10 and 30 nM concentrations (P<0.0001) when NaN3 was present (Figure 7a). Comparison with ANOVA found that Wm's concentration had a significant effect (P<0.001) on both adhesion and motility. Also, each dose of Wm significantly inhibited (P<0.01, student's t-tests) adhesion compared to levels for control cells, with or without NaN3 also present. No significant interactions between NaN3 and Wm concentration were detected for either cell function with ANOVA. Greater effects of Wm on migration compared to adhesion in glycolytic cells may be explained by the favorable contribution of deadhesion, as well as adhesion, to cell migration.
Effect of Restoring PTEN's Regulation of the PI3K/Akt Pathway on Glycolytic Cell Migration
Following Ad-PTEN transfections, at least 80% of the U87 cells exhibited GFP. Although adenoviral transfections nonspecifically inhibited cell migration, most transfections permitted sufficient Ad-control-infected cell migration to meet the criteria for evaluation and were analyzed for the effects of restoring PTEN on glycolytic migration. Each time PTEN was restored in U87 cells, their exposure to NaN3 led to significantly decreased (P<0.002) migration down to 45.7% (95% CI=41.2–50.2%) of cell migration levels without mitochondrial inhibition, as shown in four assays (Figure 8). ANOVA also demonstrated significantly decreased (P<0.0001) glycolytic migration (NaN3) in cells with restored PTEN while controlling for baseline variation among the assays. Immunoblots of U87 cells infected with Ad-PTEN or Ad-control showed that when PTEN was restored, phosphorylations of Akt and GSK3 isoforms were strongly inhibited (Figure 8b). Although the stimulatory effect of NaN3 on the pathway persisted in PTEN-restored cells, it was less powerful. The stimulatory effect of NaN3 on phosphorylation of GSK3 α and β isoforms in Ad-control-infected cells was 1.82 × and 1.98 × greater, respectively, than in cells infected with Ad-PTEN (not shown).
In two assays when freshly plated U87 cells retained their spindled morphology for 5 h in NaN3, conditioned media of Ad-PTEN-infected cells contained significantly (P=0.02) greater levels of lactic acid compared to Ad-control cells, 118.2% (95% CI=110.2–126.2%). In a third assay with numerous rounded cells present after 5 h, the difference in lactic acid levels was no longer present, presumably due to the activity of lactate dehydrogenase released from cells undergoing cell death more easily in the presence of PTEN.
Inhibition by Metformin (MF) of Glycolytic Cell Migration
An inhibitor of gluconeogenesis, MF, also suppressed U87 cell migration when cells were forced to be glycolytic by adding NaN3. Significant inhibitions (P<0.02) were obtained for U87 cells exposed to MF, 1 and 3 mM, during migration assays, with and without 1 mM MF in tissue culture during a previous overnight incubation. Cells exposed to MF, 1 mM, overnight and during the assays, migrated at 66.2% (95% CI=57.8–74.6%) of the average glycolytic cell migration (Figure 9). Comparisons with ANOVA showed that MF treatments during the assay and overnight prior to the assay were significant, P<0.001 and 0.0001, respectively, with synergy for both MF exposures, P=0.02.
This study demonstrated the capacity of malignant astrocytes to maintain survival and energy for adhesion, migration through porous filters, and invasion of Matrigel, under conditions when only glycolytic ATP was available. Suppression of mitochondrial respiration for 5 h also did not interfere with the clonogenic potential of PTEN-mutated cells. Reliance on glycolysis is forced to occur in vivo whenever mitochondrial respiration is blocked by hypoxia, mutational loss of mitochondrial proteins, or a mitochondrial inhibitor. Regions of hypoxia are commonly found in malignant gliomas and viability of primary astrocytoma cells cultured in 0.6% oxygen has verified their resistance to hypoxia.55 In this study, the in vitro motility of U87 cells persisted in 1% O2 and when mitochondrial production of ATP was maximally inhibited by NaN3. Upregulated glycolytic production of ATP energized tumor cells as they migrated in the small wells of Boyden chambers, without external removal of lactic acid, for several hours. Although mitochondrial inhibition was progressively increased to force maximal reliance on glycolysis, the rise in extracellular lactate produced by U87 cells was only a small plateau, despite the high density (1000 cells/μl) and limited buffering capacity of PBS. By default, the control of lactate in these assays was accomplished by cell metabolism.
Storage of lactate's carbon skeletons via temporary incorporation into glycogen is a rapid adaptation in gluconeogenic cells mediated by activation (dephosphorylation) of GS. A complex hierarchy of kinases and phosphatases control GS, with GSK3 providing the major share of constitutive inhibitory control. GSK3's deactivating phosphorylation of GS declines when the PI3K/Akt pathway is stimulated by growth factors, insulin, mitochondrial inhibition, or true hypoxia, especially when PTEN is lost due to mutation. Decreased expression of PTEN was found in 29 of 42 (69%) of glioblastomas from human patients based on the immunostaining of the majority of the tumor cells compared with endothelial cells and neurons as internal controls.28 On immunoblots, levels of PTEN were decreased in all six glioblastomas tested and levels of downstream phosphorylated (activated) Akt were increased in four of the six glioblastomas compared to samples of normal brain.56 Recently, using immunohistochemistry on patient tumor specimens, phosphorylated Akt has been found in 18 of 29 (62%) glioblastomas and 22 of 40 (55%) gliomas of any grade. Interestingly, none of the 22 gliomas with high levels of phosphorylated Akt, indicating an activated PI3K/Akt pathway, responded to treatment with an inhibitor of epidermal growth factor signaling. However, eight of the 18 tumors with a less active PI3K/Akt pathway, shown by low levels of phosphorylated Akt, did respond to the treatment. The level of pathway activation in the specimens was also associated with tumor progression.57
Other studies have also found that PI3K/Akt pathway signaling to inactivate GSK3 is increased in hypoxic conditions.58, 59, 60, 61 Signaling in other pathways also leads to inactivation of GSK3.62, 63, 64 The increased potential for GS activation and glycogenesis allowed by inactivated GSK3 is advantageous for tumors since the capacity for glycolytic energy production is increased whenever cells encounter hypoxia or ischemia. The buildup of lactic acid that normally shuts down glycolysis is avoided and the energy potential of carbon bonds in lactate can be preserved within glucosyl residues of glycogen, possibly in separate cellular compartments or regions. Glycogenesis can not only reserve glucose supplies for hypoxic exposures, it may also aid invasion by preventing lactic acidosis in hypoxic pseudopodia or whole cells disconnected from the vasculature and retaining lactate's carbon skeleton within glucosyl residues. Glycosyl residues can readily be utilized during aerobic energy production in another region of the cell, or on site when vascular contact is restored. By not releasing lactate extracellularly, the energy debt generated by gluconeogenesis to prevent lethal levels of lactic acidosis can be repaid during mitochondrial respiration, either elsewhere in a cell or on site at a later time.
Although the message level for GS was not altered during inhibition of mitochondria, enhanced promotion of GS activity stimulated by growth factors in PTEN-mutated cells may be coordinated with other adaptations to hypoxia and ischemia that are mediated by transcription factors responding to growth factors. Not only were message levels of fos and egr-1 elevated in hypoxia (including U87 cells in this study), they were also elevated in astrocytic cells stimulated by trophic factors.65, 66, 67, 68, 69 When egr-1 was expressed in glioblastoma cells by others, tumor cell adhesion and migration were greatly increased.70 Autocrine growth factors in gliomas may strengthen adaptation to hypoxia through fos and egr-1 as well as through GS activation that is permitted by the stimulated PI3K/Akt pathway.
Although the potential for glioma cells to avoid lactic acidosis when disconnected from the vasculature suggests a mechanism for these cells to avoid exposure to drugs delivered by the bloodstream, the U87 cells displayed susceptibility to drugs that could prevent the initiation of glycolytic-dependent migration. In earlier studies, glycolytic migration of melanoma cells was susceptible to oxamate, an inhibitor of lactate dehydrogenase,2 but systemic inhibitory levels of oxamate are difficult to achieve.71, 72, 73 In this study, U87 glioma cell migration during mitochondrial inhibition was suppressed by 2DG, MF, and Wm. Restoration of PTEN to inhibit the PI3K/Akt pathway also suppressed glycolytic cell migration.
In summary, at least two glioma cell lines exhibited the capacity to migrate under hypoxic conditions. For the U87 glioma cell line with mutational loss of PTEN, the complex metabolic response that prevents lactic acidosis within astrocytic cells was intact and stronger under hypoxic conditions than it was when PTEN was restored. The in vitro invasive activities allowed by enhanced glycolytic energy production in PTEN-mutated glioma cells observed in this study may help to explain the aggressive behavior of PTEN-mutated glioblastomas in vivo.
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We thank Jeffrey L Brodsky, PhD, Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA, USA for helpful comments and suggestions. We thank Kenneth M Yamada, MD, PhD, NIDCR, NIH, Bethesda, MD, USA for providing the PTEN construct. We acknowledge Karen B Lassige, Susan L Gleixner, and Beth Arnold, Department of Neurosurgery, University of Pittsburgh, for their technical assistance and Douglas E Moul, MD, MPH, Department of Psychiatry, University of Pittsburgh School of Medicine, for performance of ANOVA. We gratefully acknowledge funding from The Nick Eric Wichman Foundation (Ellicott City, MD), The Walter L Copeland Fund for Cranial Research of the Pittsburgh Foundation, and the American Heart Association, Ohio Valley Affiliate (AHA 9960398V).
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Beckner, M., Gobbel, G., Abounader, R. et al. Glycolytic glioma cells with active glycogen synthase are sensitive to PTEN and inhibitors of PI3K and gluconeogenesis. Lab Invest 85, 1457–1470 (2005). https://doi.org/10.1038/labinvest.3700355
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