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Effects of activation-defective TBP mutations on transcription initiation in yeast

Nature volume 369, pages 252–255 (1994)Cite this article

Abstract

TRANSCRIPTION initiation by RNA polymerase II is effected by an ordered series of general factor interactions with core promoter elements (leading to basal activity) and further regulated by gene-specific factors acting from distal elements1. Both the general factor TFIID (refs 2,3), including the constituent TBP (TATA-binding polypeptide)4–7 and associated factors8, and the interacting factor TFIIB (refs 9–11) have been implicated as targets for various activators. Towards an understanding of the basis for activator function, including the multiplicity of TBP interactions, we have now identified mutations in yeast TBP that selectively block activator (GAL4-VP16)-dependent but not basal transcription. We further show an effect of GAL4-VP16 on TFIIB recruitment to early preinitiation complexes, and that recruitment is disrupted by TBP mutations that impair its interactions with VP16 (L114K), TFIIB (L189K) or an unidentified component (K211L). Thus, GAL4-VP16 function seems to involve both direct interactions with TBP and a corresponding induction (or stabilization) of an activation-specific TBP–TFIIB–promoter complex.

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Author notes

  1. Masami Horikoshi

    Present address: Institute of Applied Microbiology, University of Tokyo, Bunkyo-ku, Tokyo, 113, Japan

Authors and Affiliations

  1. Laboratory of Biochemistry and Molecular Biology, Rockefeller University, New York, New York, 10021, USA

    Tae Kook Kim, Shigeru Hashimoto, Masami Horikoshi & Robert G. Roeder

  2. Department of Cell Biology, Stanford University School of Medicine, Stanford, California, 94305, USA

    Raymond J. Kelleher III, Peter M. Flanagan & Roger D. Kornberg

Authors
  1. Tae Kook Kim
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  2. Shigeru Hashimoto
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  3. Raymond J. Kelleher III
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  4. Peter M. Flanagan
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  5. Roger D. Kornberg
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  6. Masami Horikoshi
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  7. Robert G. Roeder
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Kim, T., Hashimoto, S., Kelleher, R. et al. Effects of activation-defective TBP mutations on transcription initiation in yeast. Nature 369, 252–255 (1994). https://doi.org/10.1038/369252a0

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