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Anatomy of a DNA replication fork revealed by reconstitution of SV40 DNA replication in vitro

Abstract

Complete enzymatic replication of DNA from the simian virus 40 origin has been reconstituted with T antigen and highly purified cellular proteins. DNA polymerase-α/primase functions primarily to synthesize RNA-DNA primers for initiation of DNA replication at the origin and for priming each Okazaki fragment. A polymerase switching mechanism requiring replication factor C and the proliferating cell nuclear antigen allows two molecules of DNA polymerase-δ to replicate both strands of the double helix conjointly.

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Waga, S., Stillman, B. Anatomy of a DNA replication fork revealed by reconstitution of SV40 DNA replication in vitro. Nature 369, 207–212 (1994). https://doi.org/10.1038/369207a0

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