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Formation of amide bonds without a condensation agent and implications for origin of life

Abstract

AMIDE bonds are of central importance for biochemistry; in the guise of peptide bonds, they form the backbone of proteins. The formation of amide bonds without the assistance of enzymes poses a major challenge for theories of the orgin of life. Enzyme-free formation of amide bonds between amino acids has been demonstrated in the presence of condensing agents such as cyanamide1–4. Here we report the formation of amide bonds in aqueous solution in the absence of any condensing agent. We find that the formation of pyrite (FeS2) from FeS and H2S can provide the driving force for reductive acetylation of amino acids with mercaptoacetic acid (HSCH2COOH). The redox energy of pyrite formation permits the activation of the carboxylic acid group, which is converted to a species that reacts readily with amines. This process provides support for the chemo-autotrophic theory5–8 for the origin of life, in which pyrite formation supplies the energy source for the first autocatalytic reproduction cycle.

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References

  1. Oró, J. Ann. N. Y. Acad. Sci. 108, 464–481 (1963).

    Article  ADS  Google Scholar 

  2. Hulshof, J. & Ponnamperuma, C. Origins Life 7, 197–224 (1976).

    Article  ADS  CAS  Google Scholar 

  3. Hawker, J. R. & Oró, J. in Origins of Life (ed. Wolman, Y.) 225–232 (Reidel, Dordrecht, 1981).

    Book  Google Scholar 

  4. Brack, A. Pure appl. Chem. 65, 1143–1151 (1993).

    Article  CAS  Google Scholar 

  5. Wächtershäuser, G. Syst. appl. Microbiol. 10, 207–210 (1988).

    Article  Google Scholar 

  6. Wächtershäuser, G. Microbiol. Rev. 52, 452–484 (1988).

    PubMed  PubMed Central  Google Scholar 

  7. Wächtershäuser, G. Proc. natn. Acad. Sci. U.S.A. 87, 200–204 (1990).

    Article  ADS  Google Scholar 

  8. Wächtershäuser, G. Prog. Biophys. molec. Biol. 58, 85–201 (1992).

    Article  Google Scholar 

  9. Gilbert, W. Nature 319, 618– (1986).

    Article  ADS  Google Scholar 

  10. Joyce, G. F. Nature 338, 217–224 (1989).

    Article  ADS  CAS  Google Scholar 

  11. DeDuve, C. Blueprint for a Cell (Neill Patterson, Burlington, North Carolina, 1991).

    Google Scholar 

  12. Drobner, E., Huber, H., Wächtershäuser, G., Rose, D. & Stetter, K. O. Nature 346, 742–744 (1990).

    Article  ADS  CAS  Google Scholar 

  13. Blöchl, E., Keller, M., Wächtershäuser, G. & Stetter, K. O. Proc. natn. Acad. Sci U.S.A. 89, 8117–8120 (1992).

    Article  ADS  Google Scholar 

  14. Strehler, B. L., Schmid, P., Li, M. P., Martin, K. & Fliss, H. J. molec. Evol. 19, 1–8 (1982).

    Article  ADS  CAS  Google Scholar 

  15. Weber, A. L. Origins Life 15, 17–27 (1984).

    Article  ADS  CAS  Google Scholar 

Download references

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Keller, M., Blöchl, E., Wächtershäuser, G. et al. Formation of amide bonds without a condensation agent and implications for origin of life. Nature 368, 836–838 (1994). https://doi.org/10.1038/368836a0

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