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S proteins control rejection of incompatible pollen in Petunia inflata


FLOWERING plants have evolved various stratagems to prevent inbreeding and promote outcrosses1. One such mechanism, gametophytic self-incompatibility, provides a genetic barrier to self-fertilization, and in the simplest cases is controlled by the highly polymorphic S locus2. Growth of a pollen tube in the style is arrested when the S allele carried by the pollen matches one of the two S alleles carried by the pistil. Putative S allele proteins of the pistil have been identified in several solanaceous species based on their co-segregation with S alleles3–12, and they have been shown to be ribonucleases13–15. So far, there has been only correlative or indirect evidence for the claim that these S allele-associated proteins (S proteins) are involved in recognition and rejection of self pollen16,17. Here we show that inhibition of synthesis of S3 and S2 proteins in Petunia inflata plants of S2S3 genotype by the antisense S3 gene resulted in failure of the transgenic plants to reject S3 and S2 pollen. We further show that expression of the transgene encoding S3 protein in P. inflata plants of S1S2 genotype confers on the transgenic plants the ability to reject S3 pollen. The self-incompatibility behaviour of the pollen was not affected by the transgene in either set of experiments. Taken together, these find-ings provide direct in vivo evidence that S proteins control the self-incompatibility behaviour of the pistil.

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  1. Frankel, R. & Galun, E. in Monographs on Theoretical and Applied Genetics (eds Frankel, R., Gall, G. A. E. & Linskens, H. F.) 5–50 (Springer, Berlin, 1977).

    Google Scholar 

  2. de Nettancourt, D. in Monographs on Theoretical and Applied Genetics (eds Frankel, R., Gall, G. A. E. & Linskens, H. F.) 28–57 (Springer, Berlin, 1977).

    Google Scholar 

  3. Bredemeijer, G. M. M. & Blass, J. Theor. appl. Genet. 59, 185–190 (1981).

    Article  CAS  Google Scholar 

  4. Anderson, M. A. et al. Nature 321, 38–44 (1986).

    Article  ADS  CAS  Google Scholar 

  5. Kamboj, R. K. & Jackson, J. F. Theor. appl. Genet. 71, 815–819 (1986).

    Article  CAS  Google Scholar 

  6. Mau, S. L. et al. Planta 169, 184–191 (1986).

    Article  CAS  Google Scholar 

  7. Broothaerts, W. J. et al. Pl. molec. Biol. 14, 93–102 (1989).

    Article  Google Scholar 

  8. Kirch, H.-H., Uhrig, H., Lottspeich, F., Salamini, F. & Thompson, R. D. Theor. appl. Genet. 78, 581–588 (1989).

    Article  CAS  Google Scholar 

  9. Ai, Y. et al. Sex. Pl. Reprod. 3, 130–138 (1990).

    Google Scholar 

  10. Kheyr-Pour, A. et al. Sex. Pl. Reprod. 3, 88–97 (1990).

    Google Scholar 

  11. Xu, B., Grun, P., Kheyr-Pour, A. & Kao, T.-h. Sex. Pl. Reprod. 3, 54–60 (1990).

    Google Scholar 

  12. Ai, Y., Kron, E. & Kao, T.-h. Molec. gen. Genet. 230, 353–358 (1991).

    Article  CAS  Google Scholar 

  13. McClure, B. A. et al. Nature 342, 955–957 (1989).

    Article  ADS  CAS  Google Scholar 

  14. Singh, A., Ai, Y. & Kao, T.-h. Pl. Physiol. 96, 61–68 (1991).

    Article  CAS  Google Scholar 

  15. Broothaerts, W., Vanvinckenroye, P., Decock, B., Van Damme, J. & Vendrig, J. C. Sex. Pl. Reprod. 4, 258–266 (1991).

    Article  Google Scholar 

  16. Singh, A. & Kao, T.-h. in Sexual Reproduction in Flowering Plants (eds Russell, S. D. & Dumas, C. ) 449–483 (Academic, San Diego, 1992).

    Google Scholar 

  17. Sims, T. L. Crit. Rev. Pl. Sci. 12, 129–167 (1993).

    Google Scholar 

  18. Coleman, C. E. & Kao, T.-h. Pl. molec. Biol. 18, 725–737 (1992).

    Article  CAS  Google Scholar 

  19. Clark, K. R., Okuley, J. J., Collins, P. D. & Sims, T. L. Pl. Cell 2, 815–826 (1990).

    Article  CAS  Google Scholar 

  20. Lewis, D. Heredity 3, 339–355 (1949).

    Article  CAS  Google Scholar 

  21. Horsch, R. B. et al. Science 227, 1229–1231 (1985).

    Article  ADS  CAS  Google Scholar 

  22. Linsmaier, E. M. & Skoog, F. Physiol. Pl. 18, 100–127 (1965).

    Article  CAS  Google Scholar 

  23. Lee, H.-S., Singh, A. & Kao, T.-h. Pl. molec. Biol. 20, 1131–1141 (1992).

    Article  CAS  Google Scholar 

  24. Oeller, P. W., Min-Wong, L., Taylor, L. P., Pike, D. A. & Theologis, A. Science 254, 437–439 (1991).

    Article  ADS  CAS  Google Scholar 

  25. loerger, T. R., Gohlke, J. R., Xu, B. & Kao, T.-h. Sex. Pl. Reprod. 4, 81–87 (1991).

    Google Scholar 

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Lee, HS., Huang, S. & Kao, Th. S proteins control rejection of incompatible pollen in Petunia inflata. Nature 367, 560–563 (1994).

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