Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

ATP-dependent nucleosome disruption at a heat-shock promoter mediated by binding of GAGA transcription factor

Nature volume 367, pages 525–532 (1994)Cite this article

Abstract

Genetic control elements are usually situated in local regions of chromatin that are hypersensitive to structural probes such as DNase I. We have reconstructed the chromatin structure of the hsp70 promoter using an in vitro nucleosome assembly system. Binding of the GAGA transcription factor on existing nucleosomes leads to nucleosome disruption, DNase I hypersensitivity at the TATA box and heat-shock elements, and rearrangement of adjacent nucleosomes. ATP hydrolysis facilitates this process, suggesting that an energy-dependent pathway is involved in chromatin remodelling.

This is a preview of subscription content

Access options

Buy article

Get time limited or full article access on ReadCube.

$32.00

Buy

All prices are NET prices.

References

  1. Felsenfeld, G. Nature 335, 219–224 (1992).

    ADS  Article  Google Scholar 

  2. Grunstein, M. Rev. Cell. Biol. 6, 643–678 (1990).

    CAS  Article  Google Scholar 

  3. Kornberg, R. D. & Lorch, Y. A. Rev. Cell. Biol. 8, 563–587 (1992).

    CAS  Article  Google Scholar 

  4. Wu, C. Nature 286, 854–860 (1980).

    ADS  CAS  Article  Google Scholar 

  5. Gross, D. S. & Garrard, W. T. A. Rev. Biochem. 57, 159–197 (1988).

    CAS  Article  Google Scholar 

  6. Workman, J. L. & Buchman, A. R. Trends biochem. Sci. 18, 90–95 (1993).

    CAS  Article  Google Scholar 

  7. Hayes, J. J. & Wolffe, A. P. Bioessays 14, 597–603 (1992).

    CAS  Article  Google Scholar 

  8. Becker, P. B. & Wu, C. Molec. cell. Biol. 12, 2241–2249 (1992).

    ADS  CAS  Article  Google Scholar 

  9. Lis, J. T. & Wu, C. Cell 74, 1–4 (1993).

    CAS  Article  Google Scholar 

  10. Soeller, W. & Kornberg, T. Molec. cell. Biol. 13, 7961–7970 (1993).

    CAS  Article  Google Scholar 

  11. Biggin, M. & Tjian, R. Cell 53, 699–711 (1988).

    CAS  Article  Google Scholar 

  12. Dynlacht, B. D., Hoey, T. & Tjian, R. T. Cell 66, 563–576 (1991).

    CAS  Article  Google Scholar 

  13. Rougvie, A. E. & Lis, J. T. Cell 54, 795–804 (1988).

    CAS  Article  Google Scholar 

  14. Rasmussen, E. & Lis, J. T. Proc. natn. Acad. Sci. U.S.A. 90, 7923–7927 (1993).

    ADS  CAS  Article  Google Scholar 

  15. Gilmour, D. S., Thomas, G. H. & Elgin, S. C. R. Science 245, 1487–1490 (1989).

    ADS  CAS  Article  Google Scholar 

  16. Wu, C. Nature 317, 84–87 (1985).

    ADS  CAS  Article  Google Scholar 

  17. Purnell, B. A. & Gilmour, D. S. Molec. cell. Biol. 13, 2593–2603 (1993).

    CAS  Article  Google Scholar 

  18. Parker, C. S. & Topol, J. Cell 36, 357–369 (1984).

    CAS  Article  Google Scholar 

  19. van Holde, K. E. Chromatin 16–30 (Springer, New York, 1989).

    Google Scholar 

  20. Wu, C. Nature 309, 229–234 (1984).

    ADS  CAS  Article  Google Scholar 

  21. Elgin, S. C. R. & Hood, L. E. J. biol. Chem. 12, 4984–4991 (1973).

    CAS  Google Scholar 

  22. Blackman, R. K. & Meselson, M. J. molec. Biol. 188, 499–515 (1986).

    CAS  Article  Google Scholar 

  23. Ashburner, M. in Developmental Studies on Giant Chromosomes (Springer, Berlin, 1972).

    Google Scholar 

  24. Burtis, K. C. et al. Cell 61, 85–89 (1990).

    CAS  Article  Google Scholar 

  25. Lue, Q., Wallrath, L. L., Granok, H. & Eigin, S. C. R. Molec. cell. Biol. 13, 2802–2814 (1993).

    Article  Google Scholar 

  26. Kerrigan, L. A., Croston, G. E., Lira, L. & Kadonaga, J. T. J. biol. Chem. 266, 574–582 (1991).

    CAS  PubMed  Google Scholar 

  27. Harrison, S. D. & Travers, A. A. EMBO J. 9, 207–216 (1990).

    CAS  Article  Google Scholar 

  28. Read, D. & Manley, J. L. EMBO J. 11, 1035–1044 (1992).

    CAS  Article  Google Scholar 

  29. Brown, J. L. & Wu, C. Development 117, 45–58 (1993).

    CAS  PubMed  Google Scholar 

  30. DiBello, P. R. et al. Genetics 129, 385–397 (1991).

    CAS  PubMed  PubMed Central  Google Scholar 

  31. Xue, F. & Cooley, L. Cell 72, 681–693 (1993).

    CAS  Article  Google Scholar 

  32. Peterson, C. L. & Herskowitz I. Cell 68, 573–583 (1992).

    CAS  Article  Google Scholar 

  33. Winston, F. & Carlson, M. Trends Genet. 8, 387–391 (1992).

    CAS  Article  Google Scholar 

  34. Henikoff, S. Trends biochem. Sci 18, 291–292 (1993).

    CAS  Article  Google Scholar 

  35. Tamkun, J. W. et al. Cell 68, 561–572 (1992).

    CAS  Article  Google Scholar 

  36. Almouzni G. & Wolffe, A. Expl Cell Res. 205, 1–15 (1993).

    CAS  Article  Google Scholar 

  37. Schmid, A., Fascher, K. D. & Horz W. Cell 71, 1–20 (1992).

    Article  Google Scholar 

  38. Chasman, D. I. et al. Genes Dev. 4, 503–514 (1990).

    CAS  Article  Google Scholar 

  39. Morrow, B. E., Ju, Q. & Warner, J. R. Molec. cell. Biol. 13, 1173–1182 (1993).

    CAS  Article  Google Scholar 

  40. Devlin, C., Tice-Baldwin, K., Shore, D. & Arndt K. T. Molec. cell. Biol. 11, 3642–3651 (1991).

    CAS  Article  Google Scholar 

  41. Axelrod, J. D., Reagan, M. S. & Majors, J. Genes Dev. 7, 857–869 (1993).

    CAS  Article  Google Scholar 

  42. Workman, J. L. & Kingston, R. E. Science 258, 1780–1784 (1992).

    ADS  CAS  Article  Google Scholar 

  43. Workman, J. L., Taylor, I. A. C. & Kingston R. E. Cell 64, 533–544 (1991).

    CAS  Article  Google Scholar 

  44. Kamakaka, R. T., Bulger, M. & Kadonaga, J. T. Genes Dev. 7, 1779–1795 (1993).

    CAS  Article  Google Scholar 

  45. Lorch, Y., LaPointe, J. W. & Kornberg, R. D. Genes Dev. 6, 2282–2287 (1992).

    CAS  Article  Google Scholar 

  46. Perlman, T. & Wragne, O. EMBO J. 7, 3073–3083 (1988).

    Article  Google Scholar 

  47. Pina, B., Bruggemeier, u. & Beato, M. Cell 60, 719–731 (1990).

    CAS  Article  Google Scholar 

  48. Archer, T. K. et al. Molec. cell. Biol. 11, 688–698 (1991).

    CAS  Article  Google Scholar 

  49. Mason, P. J. et al. J. molec. Biol. 156, 21–35 (1982).

    CAS  Article  Google Scholar 

  50. Croston, G. E., Lira, L. M. & Kadonaga, J. T. Prot. Expr. Purific 2, 162–169 (1991).

    CAS  Article  Google Scholar 

  51. Westwood, J. T., Clos, J., & Wu, C. Nature 353, 822–827 (1991).

    ADS  CAS  Article  Google Scholar 

Download references

Author information

Author notes

  1. Peter B. Becker

    Present address: Gene Expression Programme, EMBL, Meyerhofstrasse 1, D-69117, Heidel-berg, Germany

Affiliations

  1. Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health, Building 37, Room 4C-09, Bethesda, Maryland, 20892, USA

    Toshio Tsukiyama, Peter B. Becker & Carl Wu

Authors
  1. Toshio Tsukiyama
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Peter B. Becker
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Carl Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Tsukiyama, T., Becker, P. & Wu, C. ATP-dependent nucleosome disruption at a heat-shock promoter mediated by binding of GAGA transcription factor. Nature 367, 525–532 (1994). https://doi.org/10.1038/367525a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/367525a0

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing