Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

Molecular cloning of Drosophila TFIID subunits

Nature volume 367, pages 484–487 (1994)Cite this article

Abstract

TRANSCRIPTION initiation factor TFIID is a multisubunit complex containing a TATA-box-binding factor (TFIIDτ/TBP) and associated polypeptide factors (TAFs) with sizes ranging from Mr ∼20,000 to >200,000 (refs 1–7). As a result of direct promoter interactions8,9, TFIID nucleates the assembly of RNA polymerase II and other initiation factors into a functional preinitiation complex10. Although the native TFIID complex mediates both basal and activator-dependent transcription in reconstituted systems, TBP itself is competent for only basal transcription. Thus, TAFs are essential cofactors for regulated transcription. The complementary DNAs encoding the p230 (Mr 230,000), p110 and p85 subunits of TFIID have recently been cloned11–18. Here we report the molecular cloning and characterization of the p62, p42, p28 and p22 subunits. These participate in a network of heterogeneous protein–protein interactions within TFIID. Sequence similarities between p62/p42 and the histones H4/H3, respectively, suggest that these subunits have a functional relationship with chromatin.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Dynlacht, B. D., Hoey, T. & Tjian, R. Cell 66, 563–576 (1991).

    Article  CAS  Google Scholar 

  2. Tanese, N., Pugh, B. F. & Tjian, R. Genes Dev. 5, 2212–2224 (1991).

    Article  CAS  Google Scholar 

  3. Takada, R. et al. Proc. natn. Acad. Scl. U.S.A. 89, 11809–11813 (1992).

    Article  ADS  CAS  Google Scholar 

  4. Zhou, Q., Lieberman, P. M., Boyer, T. G. & Berk, A. J. Genes Dev. 6, 1964–1974 (1992).

    Article  CAS  Google Scholar 

  5. Brou, C. et al. EMBO J. 12, 489–499 (1993).

    Article  CAS  Google Scholar 

  6. Chiang, C.-M., Ge, H., Wang, Z., Hoffmann, A. & Roeder, R. G. EMBO J. 12, 2749–2762 (1993).

    Article  CAS  Google Scholar 

  7. Kokubo, T. et al. J. biol. Chem. 268, 17554–17558 (1993).

    CAS  PubMed  Google Scholar 

  8. Sawadogo, M. & Roeder, R. G. Cell 43, 165–175 (1985).

    Article  CAS  Google Scholar 

  9. Nakajima, N., Horikoshi, M. & Roeder, R. G. Molec. cell. Biol. 8, 4028–4040 (1988).

    Article  CAS  Google Scholar 

  10. Van Dyke, M. W., Roeder, R. G. & Sawadogo, M. Science 241, 1335–1338 (1988).

    Article  ADS  CAS  Google Scholar 

  11. Kokubo, T. et al. Genes Dev. 7, 1033–1046 (1993).

    Article  CAS  Google Scholar 

  12. Weinzierl, R. O. J., Dynlacht, B. D. & Tjian, R. Nature 362, 511–517 (1993).

    Article  ADS  CAS  Google Scholar 

  13. Hisatake, K. et al. Nature 362, 179–181 (1993).

    Article  ADS  CAS  Google Scholar 

  14. Ruppert, S., Wang, E. H. & Tjian, R. Nature 362, 175–179 (1993).

    Article  ADS  CAS  Google Scholar 

  15. Kokubo, T., Gong, D.-W., Roeder, R. G., Horikoshi, M. & Nakatani, Y. Proc. natn. Acad. Sci. U.S.A. 90, 5896–5900 (1993).

    Article  ADS  CAS  Google Scholar 

  16. Hoey, T. et al. Cell 72, 247–260 (1993).

    Article  CAS  Google Scholar 

  17. Kokubo, T. et al. Molec. cell. Biol. 13, 7859–7863 (1993).

    Article  CAS  Google Scholar 

  18. Dynlacht, B. D., Weinzierl, R. O. J., Admon, A. & Tjian, R. Nature 363, 176–179 (1993).

    Article  ADS  CAS  Google Scholar 

  19. Struhl, K. Molec cell. Biol. 6, 3847–3853 (1986).

    Article  CAS  Google Scholar 

  20. Gash, A., Hoffmann, A., Horikoshi, M. & Roeder, R. G. Nature 346, 390–394 (1990).

    Article  ADS  Google Scholar 

  21. Horikoshi, M., Hai, T., Lin, Y. S., Green, M. R. & Roeder, R. G. Cell 54, 1033–1042 (1988).

    Article  CAS  Google Scholar 

  22. Nakatani, Y. et al. Nature 348, 86–88 (1990).

    Article  ADS  CAS  Google Scholar 

  23. Hayes, J. J., Clark, D. J. & Wolffe, A. P. Proc. natn. Acad. Sci. U.S.A. 88, 6829–6833 (1991).

    Article  ADS  CAS  Google Scholar 

  24. Arents, G., Burlingame, R. W., Wang, B.-C., Love, W. E. & Moudrianakis, E. N. Proc. natn. Acad. Sci. U.S.A. 88, 10148–10152 (1991).

    Article  ADS  CAS  Google Scholar 

  25. Wolffe, A. P. & Dimitrov, S. Crit. Rev. euk. Gene Exp. 3, 167–191 (1993).

    CAS  Google Scholar 

  26. Tabassum, R., Sandman, K. M. & Reeve, J. N. J. Bact. 174, 7890–7895 (1992).

    Article  CAS  Google Scholar 

  27. Workman, J. L. & Roeder, R. G. Cell 51, 613–622 (1987).

    Article  CAS  Google Scholar 

  28. Workman, J. L., Abmayr, S. M., Cromlish, W. A. & Roeder, R. G. Cell 55, 211–219 (1988).

    Article  CAS  Google Scholar 

  29. Wooton, J. C. & Federhen, S. Comp. Chem. 17, 149–163 (1993).

    Article  Google Scholar 

  30. Altschul, S. F., Gish, W., Miller, W., Myers, E. W. & Lipman, D. J. J. molec. Biol. 215, 403–410 (1990).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, 20892, USA

    Tetsuro Kokubo & Yoshihiro Nakatani

  2. National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland, 20892, USA

    Tetsuro Kokubo, Da-Wei Gong & Yoshihiro Nakatani

  3. National Center for Biotechnology Information, National Institutes of Health, Bethesda, Maryland, 20892, USA

    John C. Wootton

  4. Laboratory of Biochemistry and Molecular Biology, The Rockefeller University, New York, New York, 10021, USA

    Masami Horikoshi & Robert G. Roeder

  5. Laboratory of Developmental Biology, Institute of Molecular and Cellular Biosciences, University of Tokyo, Bunkyo-ku, Tokyo, 113, Japan

    Masami Horikoshi

Authors
  1. Tetsuro Kokubo
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Da-Wei Gong
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. John C. Wootton
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Masami Horikoshi
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Robert G. Roeder
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Yoshihiro Nakatani
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kokubo, T., Gong, DW., Wootton, J. et al. Molecular cloning of Drosophila TFIID subunits. Nature 367, 484–487 (1994). https://doi.org/10.1038/367484a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/367484a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing