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Cloning and expression of cytochrome P450 genes controlling flower colour


BLUE and violet flowers generally contain derivatives of delphinidin; red and pink flowers generally contain derivatives of cyanidin or pelargonidin1.Differences in hydroxylation patterns of these three major classes of anthocyanidins are controlled by the cytochrome P450 enzymes flavonoid 3'-hydroxylase and flavonoid 3',5'-hydroxylase. Here we report on the isolation of complementary DNA clones of two different flavonoid 3',5'-hydroxylase genes that are expressed in petunia flowers. Restriction-fragment length polymorphism mapping and complementation of mutant petunia lines showed that the flavonoid 3',5'-hydroxylase genes correspond to the genetic loci Hfl and Hf2.

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Holton, T., Brugliera, F., Lester, D. et al. Cloning and expression of cytochrome P450 genes controlling flower colour. Nature 366, 276–279 (1993).

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