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Cloning and expression of cytochrome P450 genes controlling flower colour

Naturevolume 366pages276279 (1993) | Download Citation

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Abstract

BLUE and violet flowers generally contain derivatives of delphinidin; red and pink flowers generally contain derivatives of cyanidin or pelargonidin1.Differences in hydroxylation patterns of these three major classes of anthocyanidins are controlled by the cytochrome P450 enzymes flavonoid 3'-hydroxylase and flavonoid 3',5'-hydroxylase. Here we report on the isolation of complementary DNA clones of two different flavonoid 3',5'-hydroxylase genes that are expressed in petunia flowers. Restriction-fragment length polymorphism mapping and complementation of mutant petunia lines showed that the flavonoid 3',5'-hydroxylase genes correspond to the genetic loci Hfl and Hf2.

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References

  1. 1

    Forkmann, G. Pl. Breeding 106, 1–26 (1991).

  2. 2

    Dooner, H. K. & Robbins, T. P. A. Rev. Genet 25, 173–199 (1991)

  3. 3

    Stotz, G. & Forkmann, G. Z. Naturforschung 37c, 19–23 (1982).

  4. 4

    Wiering, H. Genen. Phaenen. 17, 117–134 (1974).

  5. 5

    Menting, J. G. T. thesis, La Trobe Univ. (1992).

  6. 6

    Riviere, J.-L. & Cabanne, F. Biochimie 69, 743–752 (1987).

  7. 7

    Stewart, C. B. & Schuler, M. A. Pl. Physiol. 90, 534–541 (1989).

  8. 8

    Jervis, L. & Pierpoint, W. S. J. Biotechn. 11, 161–198 (1989).

  9. 9

    Mitzutani, M. et al. Biochem. biophys. Res. Commun. 190, 875–880 (1993).

  10. 10

    Holton, T. A. & Graham, M. W. Nucleic Acids Res. 19, 1156 (1991).

  11. 11

    Cornu, A. et al. in Genetic Maps—Locus Maps of Complex Genomes 5th edn (ed. O'Brien, S. J.), (Cold Spring Harbor Laboratory Press, New York, 1990).

  12. 12

    Wallroth, M., Gerats, A. G. M., Rogers, S. G., Fraley, R. T. & Horsch, R. B. Molec. gen. Genet. 202, 6–15 (1986).

  13. 13

    Bozak, K. R., Yu, H., Sirevag, R. & Christoffersen, R. E. Proc. natn. Acad. Sci. U.S.A. 87, 3904–3908 (1990).

  14. 14

    Nebert, D. W. et al. DNA Cell Biol. 10, 1–14 (1991).

  15. 15

    Ashikari, T. et al. Appl. Microbiol. Biotechnol. 30, 515–520 (1989).

  16. 16

    Ito, H., Fukuda, Y., Murata, K. & Kimura, A. J. Bact. 153, 163–168 (1983).

  17. 17

    Brugliera, F. et al. Pl. J. (in the press).

  18. 18

    Turpen, T. H. & Griffith, D. M. BioTechniques 4, 11–15 (1986).

  19. 19

    Oeda, K. & Kakaki, T. DNA 4, 203–210 (1985).

  20. 20

    Horsch, R. B. et al. Science 227, 1229–1231 (1985).

  21. 21

    Lazo, G. R., Pascal, A. S. & Ludwig, R. A. Bio/Technology 9, 963–967 (1991).

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Author information

Author notes

    • Diane R. Lester

    Present address: CSIRO Postharvest Laboratory, North Ryde, New South Wales, 2113, Australia

    • John G. T. Menting

    Present address: La Trobe University, Bundoora, Victoria, 3083, Australia

    • Trevor W. Stevenson

    Present address: Royal Melbourne Institute of Technology, Melbourne, 3001, Australia

Affiliations

  1. Calgene Pacific Pty Ltd, 16 Gipps Street, Collingwood, Victoria, 3066, Australia

    • Timothy A. Holton
    • , Filippa Brugliera
    • , Diane R. Lester
    • , Craig D. Hyland
    • , John G. T. Menting
    • , Chin-Yi Lu
    • , Trevor W. Stevenson
    •  & Edwina C. Cornish
  2. Suntory Ltd, 1-1-1 Wadayamadai, Shimamoto-Cho, Mishima-Gun, Osaka, 618, Japan

    • Yoshikazu Tanaka
  3. INRA, Station de génétique et d'amelioration des plantes, BV 1540, 21034, Dijon cédex, France

    • Eliane Farcy

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https://doi.org/10.1038/366276a0

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