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The reaction cycle of GroEL and GroES in chaperonin-assisted protein folding

Naturevolume 366pages228233 (1993) | Download Citation

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Abstract

The reaction mechanism of protein folding by the chaperonin GroEL and its regulator GroES has been defined. GroES and substrate protein counteract each other's effects on GroEL: whereas GroES stabilizes GroEL in the ADP-bound state, binding of unfolded polypeptide within the cavity of the GroEL cylinder triggers ADP and GroES release. Upon ADP-ATP exchange, GroES reassociates with GroEL and ATP hydrolysis discharges the bound protein for folding. Partially folded protein rebinds to the chaperonin, thus perpetuating the cycle until folding is complete.

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References

  1. 1

    Ellis, R. J. & van der Vies, S. M. A. Rev. Biochem. 60, 327–347 (1991).

  2. 2

    Gething, M. J. & Sambrook, J. Nature 355, 33–45 (1992).

  3. 3

    Hendrick, J. P. & Hartl, F.-U. A. Rev. Biochem. 62, 349–384 (1993).

  4. 4

    Hemmingsen, S. M. et al. Nature 333, 330–334 (1988).

  5. 5

    Hendrix, R. W. J. molec. Biol. 129, 375–392 (1979).

  6. 6

    Hohn, T., Hohn, B., Engel, A. & Wurtz, M. J. molec. Biol. 129, 359–373 (1979).

  7. 7

    Braig, K., Simon, M., Furnya, F., Hainfeld, J. F. & Horwich, A. L. Proc. natn. Acad. Sci. U.S.A. 90, 3978–3982 (1993).

  8. 8

    Langer, T., Pfeifer, G., Martin, J., Baumeister, W. & Hartl, F.-U. EMBO J. 11, 4757–4765 (1992).

  9. 9

    Martin, J. et al. Nature 352, 36–42 (1991).

  10. 10

    van der Vies, S. M., Viitanen, P. V., Gatenby, A. A., Lorimer, G. H. & Jaenicke, R. Biochemistry 31, 3635–3644 (1992).

  11. 11

    Bochkareva, E. S., Lissin, N. M., Flynn, G. C., Rothman, J. E. & Girshovich, A. S. J. biol. Chem. 267, 6796–6800 (1992).

  12. 12

    Gray, T. E. & Fersht, A. R. FEBS Lett. 292, 254–258 (1991).

  13. 13

    Jackson, G. S. et al. Biochemistry 32, 2554–2563 (1993).

  14. 14

    Goloubinoff, P., Christeller, J. T., Gatenby, A. A. & Lorimer, G. H. Nature 342, 884–889 (1989).

  15. 15

    Buchner, J. et al. Biochemistry 30, 1586–1591 (1991).

  16. 16

    Landry, S. J., Zeilstra-Ryalls, J., Fayet, O., Georgopoulos, C. & Gierasch, L. M. Nature 364, 255–258 (1993).

  17. 17

    Chandrasekhar, G. N., Tilly, K., Woolford, C., Hendrix, R. & Georgopoulos, C. J. biol. Chem. 261, 12414–12419 (1986).

  18. 18

    Saibil, H. R., Dong, Z., Wood, S. & auf der Mauer, A. Nature 353, 25–26 (1991).

  19. 19

    Saibil, H. R. et al. Curr. Biol. 3, 265–273 (1993).

  20. 20

    Viitanen, P. V. et al. Biochemistry 29, 5665–5671 (1990).

  21. 21

    Mendoza, J. A., Rogers, E., Lorimer, G. H. & Horowitz, P. M. J. biol. Chem. 266, 13044–13049 (1991).

  22. 22

    Martin, J., Geromanos, S., Tempst, P. & Hartl, F.-U. Nature 366, 279–282 (1993).

  23. 23

    McLennan, N. F., Girshovich, A. S., Lissin, N. M., Charters, Y. & Masters, M. Molec. Microbiol. 7, 49–58 (1993).

  24. 24

    Todd, M. J., Viitanen, P. V. & Lorimer, G. H. Biochemistry 32, 8560–8567 (1993).

  25. 25

    Creighton, T. E. Nature 352, 17–18 (1991).

  26. 26

    Viitanen, P. V. et al. J. biol. Chem. 267, 695–698 (1992).

  27. 27

    Bochkareva, E. S. & Girshovich, A. S. J. biol. Chem. 267, 25672–25675 (1992).

  28. 28

    Viitanen, P. V., Donaldson, G. K., Lorimer, G. H., Lubben, T. H. & Gatenby, A. A. Biochemistry 30, 9716–9723 (1991).

  29. 29

    Fayet, O., Louran, J. M. & Georgopoulous, C. Molec. gen. Genet. 202, 435–445 (1986).

  30. 30

    Zhi, W., Landry, S. J., Gierasch, L. M. & Srere, P. A. Prot. Sci. 1, 522–529 (1992).

  31. 31

    Shlomai, J. & Kornberg, A. J. biol. Chem. 255, 6789–6793 (1980).

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    • Thomas Langer

    Present address: Institut für Physiologische Chemie, Goethestrasse 33, 80336, Munchen, Germany

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  1. Cellular Biochemistry and Biophysics Program, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, New York, 10021, USA

    • Jörg Martin
    • , Mark Mayhew
    • , Thomas Langer
    •  & Ulrich Hartl

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https://doi.org/10.1038/366228a0

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