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Yeast origin recognition complex is involved in DNA replication and transcriptional silencing

Naturevolume 366pages8789 (1993) | Download Citation

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Abstract

THE HMR E silencer represses transcription of silent mating-type genes in the budding yeast Saccharomyces cerevisiae and contains three redundant regulatory elements A, E and B (ref. 1). The A element contains the 11 base pair consensus sequence that is essential for the firing of DNA replication origins2. A multisubunit protein called the origin recognition complex (ORC) binds specifically to this consensus sequence within yeast origins in vitro3 and in vivo4. We isolated mutants in A element-mediated silencing and report here that one of the genes we identified, RRR1, encodes ORC2, the 72K subunit of ORC. RRR1/ORC2 is an essential gene, but the rrr1-316 allele, which is viable, is defective in the replication of nuclear DNA and the maintenance of the 2-μm episomal DNA. This is, to our knowledge, the first genetic evidence that ORC is involved in DNA replication and silencing.

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Author information

Author notes

    • Gos Micklem

    Present address: Sanger Centre, Hinxton, Cambridge, CB10 IRQ, UK

  1. John F. X. Diffley: To whom correspondence should be addressed.

Affiliations

  1. MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, UK

    • Gos Micklem
  2. ICRF Clare Hall Laboratories, South Mimms, Hertfordshire, EN6 3LD, UK

    • Adele Rowley
    • , Janet Harwood
    •  & John F. X. Diffley
  3. Research Institute of Molecular Pathology, Dr Bohr Gasse 7, 1030, Vienna, Austria

    • Kim Nasmyth

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https://doi.org/10.1038/366087a0

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