By two-dimensional (2-D) DNA typing a restriction enzyme digest of genomic DNA can be resolved on the basis of both size and base-pair sequence and subsequently analysed by repeat probe hybridization to reveal sequence variants at multiple genomic sites in parallel. The system has been partly automated and allows for large-scale comparative analysis of complex genomes in a cost-effective manner.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
References
Schaap, G. H., Verkerk, A., Vijg, J. & Jongkind, J. F. Cytometry 3, 408–413 (1983).
O'Farrell, P. J. biol. Chem. 250, 4007–4021 (1975).
Scheele, G. A. J. biol. Chem. 250, 5375–5380 (1975).
Fischer, S. G. & Lerman, L. S. Cell 16, 191–200 (1979).
Uitterlinden, A. G., Slagboom, P., Knook, D. L. & Vijg, J. Proc. natn. Acad. Sci. U.S.A. 86, 2742–2746 (1989).
Uitterlinden, A. G. & Vijg, J. in Two-Dimensional DNA Typing: A Parallel Approach to Genome Analysis (Horwood, Chichester, 1993).
Hovig, E., Mullaart, E. Börresen, A. -L., Uitterlinden, A. G. & Vijg, J. Genomics 17, 66–75 (1993).
Verwest, A. M. et al. Br. J. Cancer (in the press).
te Meerman, G. J., Mullaart, E. van der Meulen, M. A., den Daas, J. H. G., Morolli, B., Uitterlinden, A. G. & Vijg, J. Am. J. hum. Genet. (in the press).
Jeffreys, A. J., Wilson, V. & Thein, S. L. Nature 314, 67–73 (1985).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Mullaart, E., de Vos, G., te Meerman, G. et al. Parallel genome analysis by two-dimensional DNA typing. Nature 365, 469–471 (1993). https://doi.org/10.1038/365469a0
Issue Date:
DOI: https://doi.org/10.1038/365469a0
This article is cited by
-
Detecting Individual Genetic Variation
Nature Biotechnology (1995)
-
Two-dimensional DNA typing
Molecular Biotechnology (1995)